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3.A.1.14.1
Iron (Fe3+) or ferric-dicitrate porter, FecBCDE (Braun and Herrmann, 2007). The Mycobacterium tuberculosis (Mtb) ortholog has two substrate binding proteins, FecB and FecB2 (de Miranda et al. 2023).  The crystallographic structures of Mtb FecB and FecB2 were determined to 2.0 Å and 2.2 Å resolution, respectively, and they show distinct ligand binding pockets. In vitro ligand binding experiments for FecB and FecB2 were performed with heme and bacterial siderophores from Mtb and other species, revealing that both FecB and FecB2 bind heme, while only FecB binds the Mtb sideophore ferric-carboxymycobactin (Fe-cMB). Subsequent structure-guided mutagenesis of FecB identified a single glutamate residue-Glu339-that significantly contributes to Fe-cMB binding (de Miranda et al. 2023).

Accession Number:P15031
Protein Name:Fe(3+) dicitrate transport ATP-binding protein FecE
Length:255
Molecular Weight:28191.00
Species:Escherichia coli (strain K12) [83333]
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Peripheral membrane protein2
Substrate

Cross database links:

Entrez Gene ID: 948819   
Pfam: PF00005   
KEGG: eco:b4287   

Gene Ontology

GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0016887 F:ATPase activity
GO:0006811 P:ion transport
GO:0055072 P:iron ion homeostasis

References (4)

[1] “Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.”  Staudenmaier H.et.al.   2651410
[2] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553

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FASTA formatted sequence
1:	MTLRTENLTV SYGTDKVLND VSLSLPTGKI TALIGPNGCG KSTLLNCFSR LLMPQSGTVF 
61:	LGDNPINMLS SRQLARRLSL LPQHHLTPEG ITVQELVSYG RNPWLSLWGR LSAEDNARVN 
121:	VAMNQTRINH LAVRRLTELS GGQRQRAFLA MVLAQNTPVV LLDEPTTYLD INHQVDLMRL 
181:	MGELRTQGKT VVAVLHDLNQ ASRYCDQLVV MANGHVMAQG TPEEVMTPGL LRTVFSVEAE 
241:	IHPEPVSGRP MCLMR