3.A.1.201.22
Mitochondrial ATP-binding cassette 1, ABCB8. Mediates doxorubicin resistance in melanoma cells (Elliott and Al-Hajj 2009). It is regulated by the Sp1 transcription factor and down regulated by mthramycin A which blocks Sp1 binding to the DNA (Sachrajda and Ratajewski 2011). It is also regulated by neuropilin-1, NRP1 (TC# 8.A.47.1.5) (Issitt et al. 2018). The cryo-EM structure of ABCB8 bound to AMPPNP in the inward-facing conformation was solved with a resolution of 4.1 Å. hABCB8 shows an open-inward conformation when ATP is bound, and cholesterol molecules were identified in the transmembrane domain of hABCB8 (Li et al. 2021).
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| Accession Number: | Q9NUT2 |
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| Protein Name: | ATP-binding cassette sub-family B member 8, mitochondrial |
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| Length: | 735 |
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| Molecular Weight: | 79989.00 |
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| Species: | Homo sapiens (Human) [9606] |
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| Number of TMSs: | 4 |
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| Location1 / Topology2 / Orientation3: |
Mitochondrion inner membrane1 / Multi-pass membrane protein2 |
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| Substrate |
doxorubicin |
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1: MLVHLFRVGI RGGPFPGRLL PPLRFQTFSA VRNTWRNGKT GQLHKAEGEY SDGYRSSSLL
61: RAVAHLRSQL WAHLPRAPLA PRWSPSAWCW VGGALLGPMV LSKHPHLCLV ALCEAEEAPP
121: ASSTPHVVGS RFNWKLFWQF LHPHLLVLGV AVVLALGAAL VNVQIPLLLG QLVEVVAKYT
181: RDHVGSFMTE SQNLSTHLLI LYGVQGLLTF GYLVLLSHVG ERMAVDMRRA LFSSLLRQDI
241: TFFDANKTGQ LVSRLTTDVQ EFKSSFKLVI SQGLRSCTQV AGCLVSLSML STRLTLLLMV
301: ATPALMGVGT LMGSGLRKLS RQCQEQIARA MGVADEALGN VRTVRAFAME QREEERYGAE
361: LEACRCRAEE LGRGIALFQG LSNIAFNCMV LGTLFIGGSL VAGQQLTGGD LMSFLVASQT
421: VQRSMANLSV LFGQVVRGLS AGARVFEYMA LNPCIPLSGG CCVPKEQLRG SVTFQNVCFS
481: YPCRPGFEVL KDFTLTLPPG KIVALVGQSG GGKTTVASLL ERFYDPTAGV VMLDGRDLRT
541: LDPSWLRGQV VGFISQEPVL FGTTIMENIR FGKLEASDEE VYTAAREANA HEFITSFPEG
601: YNTVVGERGT TLSGGQKQRL AIARALIKQP TVLILDEATS ALDAESERVV QEALDRASAG
661: RTVLVIAHRL STVRGAHCIV VMADGRVWEA GTHEELLKKG GLYAELIRRQ ALDAPRTAAP
721: PPKKPEGPRS HQHKS