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3.A.1.201.7
Auxin efflux pump Pgp4; AbcB4; MDR4; PGP4 (Lefèvre and Boutry 2018) of 1286 aas and 12 TMSs in a MCMC domain arrangement.  Functions in the basipetal redirection of auxin from the root tip. Strongly expressed in root cap and epidermal cells (Terasaka et al., 2005).  Contributes to the basipetal transport in hypocotyls and root tips by establishing an auxin uptake sink in the root cap. Confers sensitivity to 1-N-naphthylphthalamic acid (NPA). Regulates root elongation, the initiation of lateral roots and the development of root hairs. Can transport IAA, indole-3-propionic acid, NPA syringic acid, vanillic acid and some auxin metabolites, but not 2,4-D and 1-naphthaleneacetic acid (Terasaka et al., 2005). Pgps and PINs (TC# 2.A.69) function in coordinated but independent auxin transport but also function interactively in a tissue-specific manner (Blakeslee et al. 2007). Found in the plasma membranes of root hair cells (Cho et al. 2012).  ABCB4 gene mutations may be involved in amiodarone-induced hepatotoxicity (Shi et al. 2024).

Accession Number:O80725
Protein Name:Multidrug resistance protein 4 aka Pgp4
Length:1286
Molecular Weight:139028.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate auxin

Cross database links:

RefSeq: NP_182223.1   
Entrez Gene ID: 819314   
Pfam: PF00664    PF00005   
KEGG: ath:AT2G47000   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0008559 F:xenobiotic-transporting ATPase activity
GO:0010315 P:auxin efflux
GO:0010540 P:basipetal auxin transport
GO:0009630 P:gravitropism
GO:0009735 P:response to cytokinin stimulus
GO:0048767 P:root hair elongation
GO:0055085 P:transmembrane transport

References (8)

[1] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[2] “PGP4, an ATP binding cassette P-glycoprotein, catalyzes auxin transport in Arabidopsis thaliana roots.”  Terasaka K.et.al.   16243904
[3] “The Arabidopsis thaliana ABC protein superfamily, a complete inventory.”  Sanchez-Fernandez R.et.al.   11346655
[4] “Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry.”  Nuehse T.S.et.al.   14506206
[5] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[6] “MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development.”  Santelia D.et.al.   16198350
[7] “Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses.”  Nuehse T.S.et.al.   17651370
[8] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MASESGLNGD PNILEEVSET KRDKEEEEEV KKTEKKDEEH EKTKTVPFYK LFAFADSFDF 
61:	LLMILGTLGS IGNGLGFPLM TLLFGDLIDA FGENQTNTTD KVSKVALKFV WLGIGTFAAA 
121:	FLQLSGWMIS GERQAARIRS LYLKTILRQD IAFFDIDTNT GEVVGRMSGD TVLIQDAMGE 
181:	KVGKAIQLLA TFVGGFVIAF VRGWLLTLVM LSSIPLLVMA GALLAIVIAK TASRGQTAYA 
241:	KAATVVEQTI GSIRTVASFT GEKQAISNYN KHLVTAYKAG VIEGGSTGLG LGTLFLVVFC 
301:	SYALAVWYGG KLILDKGYTG GQVLNIIIAV LTGSMSLGQT SPCLSAFAAG QAAAYKMFET 
361:	IERRPNIDSY STNGKVLDDI KGDIELKDVY FTYPARPDEQ IFRGFSLFIS SGTTVALVGQ 
421:	SGSGKSTVVS LIERFYDPQA GDVLIDGINL KEFQLKWIRS KIGLVSQEPV LFTASIKDNI 
481:	AYGKEDATTE EIKAAAELAN ASKFVDKLPQ GLDTMVGEHG TQLSGGQKQR IAVARAILKD 
541:	PRILLLDEAT SALDAESERV VQEALDRIMV NRTTVVVAHR LSTVRNADMI AVIHQGKIVE 
601:	KGSHTELLKD PEGAYSQLIR LQEEKKSDEN AAEEQKMSSI ESFKQSSLRK SSLGRSLSKG 
661:	GSSRGNSSRH SFNMFGFPAG IDGNVVQDQE EDDTTQPKTE PKKVSIFRIA ALNKPEIPVL 
721:	ILGSISAAAN GVILPIFGIL ISSVIKAFFQ PPKKLKEDTS FWAIIFMVLG FASIIAYPAQ 
781:	TFFFAIAGCK LVQRIRSMCF EKVVHMEVGW FDEPENSSGT IGARLSADAA TIRGLVGDSL 
841:	AQTVQNLSSI LAGLIIAFLA CWQLAFVVLA MLPLIALNGF LYMKFMKGFS ADAKKMYGEA 
901:	SQVANDAVGS IRTVASFCAE DKVMNMYSKK CEGPMKNGIR QGIVSGIGFG FSFFVLFSSY 
961:	AASFYVGARL VDDGKTTFDS VFRVFFALTM AAMAISQSSS LSPDSSKADV AAASIFAIMD 
1021:	RESKIDPSVE SGRVLDNVKG DIELRHVSFK YPARPDVQIF QDLCLSIRAG KTVALVGESG 
1081:	SGKSTVIALL QRFYDPDSGE ITLDGVEIKS LRLKWLRQQT GLVSQEPILF NETIRANIAY 
1141:	GKGGDASESE IVSSAELSNA HGFISGLQQG YDTMVGERGI QLSGGQKQRV AIARAIVKDP 
1201:	KVLLLDEATS ALDAESERVV QDALDRVMVN RTTIVVAHRL STIKNADVIA VVKNGVIVEK 
1261:	GKHDTLINIK DGVYASLVQL HLTAAS