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3.A.1.208.20
The vacuolar MRP1 of 1622 aas. Also called ABCC1 and EST1. It has 17 TMSs in a 5 + 6 + 6 arrangement with an N-terminal transmembrane domain of 5 TMSs.  The domain order is N - MCMC, and it transports xenobiotics (Lefèvre and Boutry 2018) It sequesters in the vacuole glutathione conjugates, folate mono-glutamates (pteroyl-1-glutamate) and antifolates (methotrexate); (Raichaudhuri et al. 2009) (86% identical to MRP2 (3.A.1.208.5). ABCC1 and ABCC2 confer tolerance to cadmium and mercury, in addition to their role in arsenic detoxification. MRP1 of Lithospermum erythrorhizon may play a direct or indirect role in transmembrane transport  of shikonin (Zhu et al. 2017). ABCC1 and ABCC2 regulate stomatal closing and opening as well as anthocyanin transport (Frelet-Barrand et al. 2008). It transports xenobiotics (Lefèvre and Boutry 2018). The ABCC gene family and their expression analysis in pigment deposition of fiber in brown cotton (Gossypium hirsutum) has been reported (Sun et al. 2021).

Accession Number:Q9C8G9
Protein Name:Multidrug resistance-associated protein 1
Length:1622
Molecular Weight:181927.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:15
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Folate mono-glutamates, Antifolates

Cross database links:

RefSeq: NP_001031116.1    NP_174329.1   
Entrez Gene ID: 839920   
Pfam: PF00664    PF00005   
KEGG: ath:AT1G30400   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0000325 C:plant-type vacuole
GO:0005886 C:plasma membrane
GO:0005774 C:vacuolar membrane
GO:0005524 F:ATP binding
GO:0005516 F:calmodulin binding
GO:0008559 F:xenobiotic-transporting ATPase activity
GO:0055085 P:transmembrane transport

References (13)

[1] “AtMRP1 gene of Arabidopsis encodes a glutathione S-conjugate pump: isolation and functional definition of a plant ATP-binding cassette transporter gene.”  Lu Y.-P.et.al.   9223346
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics.”  Tommasini R.et.al.   9271206
[5] “Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana.”  Sanchez-Fernandez R.et.al.   9671034
[6] “AtMRP2, an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with AtMRP1.”  Lu Y.-P.et.al.   9490749
[7] “The Arabidopsis thaliana ABC protein superfamily, a complete inventory.”  Sanchez-Fernandez R.et.al.   11346655
[8] “Multifunctionality of plant ABC transporters -- more than just detoxifiers.”  Martinoia E.et.al.   11855639
[9] “Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana.”  Kolukisaoglu U.H.et.al.   12430019
[10] “Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters.”  Geisler M.et.al.   15133126
[11] “Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.”  Shimaoka T.et.al.   15215502
[12] “Large-scale characterization of integral proteins from Arabidopsis vacuolar membrane by two-dimensional liquid chromatography.”  Szponarski W.et.al.   14760709
[13] “Plant ABC proteins - a unified nomenclature and updated inventory.”  Verrier P.J.et.al.   18299247

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FASTA formatted sequence
1:	MGFEPLDWYC KPVPNGVWTK TVDYAFGAYT PCAIDSFVLG ISHLVLLILC LYRLWLITKD 
61:	HKVDKFCLRS KWFSYFLALL AAYATAEPLF RLVMRISVLD LDGAGFPPYE AFMLVLEAFA 
121:	WGSALVMTVV ETKTYIHELR WYVRFAVIYA LVGDMVLLNL VLSVKEYYGS FKLYLYISEV 
181:	AVQVAFGTLL FVYFPNLDPY PGYTPVGTEN SEDYEYEELP GGENICPERH ANLFDSIFFS 
241:	WLNPLMTLGS KRPLTEKDVW HLDTWDKTET LMRSFQKSWD KELEKPKPWL LRALNNSLGG 
301:	RFWWGGFWKI GNDCSQFVGP LLLNELLKSM QLNEPAWIGY IYAISIFVGV VLGVLCEAQY 
361:	FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RKKFQTGKIT NLMTTDAESL QQICQSLHTM 
421:	WSAPFRIIVA LVLLYQQLGV ASIIGALFLV LMFPIQTVII SKTQKLTKEG LQRTDKRIGL 
481:	MNEVLAAMDT VKCYAWENSF QSKVQTVRDD ELSWFRKAQL LSAFNMFILN SIPVLVTVVS 
541:	FGVFSLLGGD LTPARAFTSL SLFSVLRFPL FMLPNIITQM VNANVSLNRL EEVLSTEERV 
601:	LLPNPPIEPG QPAISIRNGY FSWDSKADRP TLSNINLDIP LGSLVAVVGS TGEGKTSLIS 
661:	AMLGELPARS DATVTLRGSV AYVPQVSWIF NATVRDNILF GAPFDQEKYE RVIDVTALQH 
721:	DLELLPGGDL TEIGERGVNI SGGQKQRVSM ARAVYSNSDV CILDDPLSAL DAHVGQQVFE 
781:	KCIKRELGQT TRVLVTNQLH FLSQVDKILL VHEGTVKEEG TYEELCHSGP LFQRLMENAG 
841:	KVEDYSEENG EAEVDQTSVK PVENGNANNL QKDGIETKNS KEGNSVLVKR EERETGVVSW 
901:	KVLERYQNAL GGAWVVMMLV ICYVLTQVFR VSSSTWLSEW TDSGTPKTHG PLFYNIVYAL 
961:	LSFGQVSVTL INSYWLIMSS LYAAKKMHDA MLGSILRAPM VFFQTNPLGR IINRFAKDMG 
1021:	DIDRTVAVFV NMFMGSIAQL LSTVILIGIV STLSLWAIMP LLVVFYGAYL YYQNTSREIK 
1081:	RMDSTTRSPV YAQFGEALNG LSSIRAYKAY DRMAEINGRS MDNNIRFTLV NMAANRWLGI 
1141:	RLEVLGGLMV WLTASLAVMQ NGKAANQQAY ASTMGLLLSY ALSITSSLTA VLRLASLAEN 
1201:	SLNSVERVGN YIEIPSEAPL VIENNRPPPG WPSSGSIKFE DVVLRYRPEL PPVLHGVSFL 
1261:	ISPMDKVGIV GRTGAGKSSL LNALFRIVEL EKGRILIDEC DIGRFGLMDL RKVLGIIPQA 
1321:	PVLFSGTVRF NLDPFSEHND ADLWESLERA HLKDTIRRNP LGLDAEVTEA GENFSVGQRQ 
1381:	LLSLARALLR RSKILVLDEA TAAVDVRTDV LIQKTIREEF KSCTMLIIAH RLNTIIDCDK 
1441:	VLVLDSGKVQ EFSSPENLLS NGESSFSKMV QSTGTANAEY LRSITLENKR TREANGDDSQ 
1501:	PLEGQRKWQA SSRWAAAAQF ALAVSLTSSH NDLQSLEIED DNSILKKTKD AVVTLRSVLE 
1561:	GKHDKEIEDS LNQSDISRER WWPSLYKMVE GLAVMSRLAR NRMQHPDYNL EGKSFDWDNV 
1621:	EM