3.A.1.3.29 Histidine/Arginine/Lysine (basic amino acid) uptake porter, HisJ/ArgT/HisP/HisM/HisQ [R, R, C, M, M, respectively] (Gilson et al. 1982). HisJ binds L-His (preferred), but 1-methyl-L-His
and 3-methyl-L-His also bind, while the dipeptide carnosine binds weakly; D-histidine and the
histidine degradation products, histamine, urocanic acid and imidazole do not bind. L-Arg,
homo-L-Arg, and post-translationally modified methylated Arg-analogs also bind with the exception of symmetric dimethylated-L-Arg. L-Lys and L-Orn show weaker interactions with HisJ and methylated and acetylated Lys variants show poor binding.The carboxylate groups of these amino acids and
their variants are essential (Paul et al. 2016).
|
Accession Number: | P0AEU0 |
Protein Name: | Histidine-binding periplasmic protein |
Length: | 260 |
Molecular Weight: | 28483.00 |
Species: | Escherichia coli (strain K12) [83333] |
Number of TMSs: | 1 |
Location1 / Topology2 / Orientation3: |
Periplasm1 |
Substrate |
lysine, arginine, histidine |
---|
1: MKKLVLSLSL VLAFSSATAA FAAIPQNIRI GTDPTYAPFE SKNSQGELVG FDIDLAKELC
61: KRINTQCTFV ENPLDALIPS LKAKKIDAIM SSLSITEKRQ QEIAFTDKLY AADSRLVVAK
121: NSDIQPTVES LKGKRVGVLQ GTTQETFGNE HWAPKGIEIV SYQGQDNIYS DLTAGRIDAA
181: FQDEVAASEG FLKQPVGKDY KFGGPSVKDE KLFGVGTGMG LRKEDNELRE ALNKAFAEMR
241: ADGTYEKLAK KYFDFDVYGG