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3.A.10.1.4
Na+-translocating PPase (Malinen et al., 2007).  The 3-d resting state structure has been solved to 2.6 Å (Kellosalo et al. 2012).  The structure shows that the hydrolytic center is 20 Å above the membrane, coupled to the gate formed by the conserved Asp(243), Glu(246) and Lys(707) by an unusual "coupling funnel" of six α-helices. Helix 12 may slide down upon substrate binding to open the gate by a simple binding-change mechanism. Below the gate, four helices form the exit channel. Superimposing helices 3 to 6, 9 to 12, and 13 to 16 suggests that this PPases arose by gene triplication (Kellosalo et al. 2012).

Accession Number:Q9S5X0
Protein Name:Pyrophosphate-energized proton pump
Length:726
Molecular Weight:77046.00
Species:Thermotoga maritima [2336]
Number of TMSs:16
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate sodium(1+)

Cross database links:

RefSeq: NP_227989.1   
Entrez Gene ID: 897014   
Pfam: PF03030   
BioCyc: TMAR243274:TM_0174-MONOMER   
KEGG: tma:TM0174   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0009678 F:hydrogen-translocating pyrophosphatase acti...
GO:0004427 F:inorganic diphosphatase activity
GO:0000287 F:magnesium ion binding
GO:0015992 P:proton transport

References (3)

[1] “Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima.”  Nelson K.E.et.al.   10360571
[2] “Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: properties and gene structure.”  Bouthier de la Tour C.et.al.   9440516
[3] “A thermostable K(+)-stimulated vacuolar-type pyrophosphatase from the hyperthermophilic bacterium Thermotoga maritima.”  Perez-Castineira J.R.et.al.   11343697
Structure:
4AV3   4AV6   5LZQ   5LZR   6QXA     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MYVAALFFLI PLVALGFAAA NFAAVVRKPE GTERMKEISS YIRSGADSFL AHETKAIFKV 
61:	AIVIAILLMI FTTWQTGVAF LLGAVMSASA GIVGMKMATR ANVRVAEAAR TTKKIGPALK 
121:	VAYQGGSVMG LSVGGFALLG LVLVYLIFGK WMGQVDNLNI YTNWLGINFV PFAMTVSGYA 
181:	LGCSIIAMFD RVGGGVYTKA ADMAADLVGK TELNLPEDDP RNPATIADNV GDNVGDVAGL 
241:	GADLLESFVG AIVSSIILAS YMFPIYVQKI GENLVHQVPK ETIQALISYP IFFALVGLGC 
301:	SMLGILYVIV KKPSDNPQRE LNISLWTSAL LTVVLTAFLT YFYLKDLQGL DVVGFRFGAI 
361:	SPWFSAIIGI FSGILIGFWA EYYTSYRYKP TQFLSKSSIE GTGMVISNGL SLGMKSVFPP 
421:	TLTLVLGILF ADYFAGLYGV AIAALGMLSF VATSVSVDSY GPIADNAGGI SEMCELDPEV 
481:	RKITDHLDAV GNTTAAIGKG FAIGSAIFAA LSLFASYMFS QISPSDIGKP PSLVLLLNML 
541:	DARVIAGALL GAAITYYFSG YLISAVTKAA MKMVDEIRRQ AREIPGLLEG KAKPDYNRCI 
601:	EITSDNALKQ MGYPAFIAIL TPLVTGFLLG AEFVGGVLIG TVLSGAMLAI LTANSGGAWD 
661:	NAKKYLEAGN LEGYGKGSEP HKALVIGDTV GDPLKDTVGP SLDILIKIMS VVSVIAVSIF 
721:	KHVHLF