3.A.29.1.4
FtsH zinc metaloprotease of 634 aas and 2 TMSs. 3-d structures have been determined (Uthoff and Baumann 2018). The active FtsH is a dodecamer, which consists of two FtsH hexamers. A tilted conformation of the cytosolic domain in the FtsH dodecamer, visualized by negative stain TEM suggests a lateral substrate entrance between the membrane and cytosolic domains. Such a substrate path was resolved in the cryo-EM structure of the FtsH hexamer (Carvalho et al. 2020).
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| Accession Number: | O67077 |
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| Protein Name: | ATP-dependent zinc metalloprotease FtsH |
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| Length: | 634 |
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| Molecular Weight: | 70686.00 |
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| Species: | Aquifex aeolicus (strain VF5) [224324] |
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| Number of TMSs: | 2 |
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| Location1 / Topology2 / Orientation3: |
Cell inner membrane1 / Multi-pass membrane protein2 / Cytoplasmic side3 |
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| Substrate |
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1: MNALKNFFIW AIIIGAAIVA FNLFEGKREF TTKVSLNEVV KLVEEGKVSY AEVRGNTAII
61: QTKDGQKLEV TLPPNTNLVD KMVEKGVRVE VANPEPPGGW LVNVFLSWLP ILFFIGIWIF
121: LLRQMSGGGN VNRAFNFGKS RAKVYIEEKP KVTFKDVAGI EEVKEEVKEI IEYLKDPVKF
181: QKLGGRPPKG VLLYGEPGVG KTLLAKAIAG EAHVPFISVS GSDFVEMFVG VGAARVRDLF
241: ETAKKHAPCI IFIDEIDAVG RARGAIPVGG GHDEREQTLN QLLVEMDGFD TSDGIIVIAA
301: TNRPDILDPA LLRPGRFDRQ IFIPKPDVRG RYEILKVHAR NKKLAKDVDL EFVARATPGF
361: TGADLENLLN EAALLAARKG KEEITMEEIE EALDRITMGL ERKGMTISPK EKEKIAIHEA
421: GHALMGLVSD DDDKVHKISI IPRGMALGVT QQLPIEDKHI YDKKDLYNKI LVLLGGRAAE
481: EVFFGKDGIT TGAENDLQRA TDLAYRMVSM WGMSDKVGPI AIRRVANPFL GGMTTAVDTS
541: PDLLREIDEE VKRIITEQYE KAKAIVEEYK EPLKAVVKKL LEKETITCEE FVEVFKLYGI
601: ELKDKCKKEE LFDKDRKSEE NKELKSEEVK EEVV