Search TCDB: (?)
TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.3.10.8
This protein, P5B-ATPase, was originally designated the functionally uncharacterized P-type ATPase 14 (FUPA14) (Thever and Saier 2009), but it has been shown to be a vacuolar ATPase, Ypk1, that functions in manganese detoxification and homeostasis (Chesi et al. 2012).  It is therefore likely to catalyze export of manganese ions from the cytoplasm into the vacuole. However, it also transporter polyamines such as spermine . The 3-D structure has been determined to 3.4 Å, and it revealed three separate transport cycle intermediates, including spermine-bound conformations (Li et al. 2021). In the absence of cargo, Ypk9 rests in a phosphorylated conformation auto-inhibited by the N-terminus. Spermine uptake into vesicles is accomplished through an electronegative cleft lined by transmembrane segments 2, 4 and 6 (Li et al. 2021).

Accession Number:Q12697
Protein Name:Probable cation-transporting ATPase 2
Length:1472
Molecular Weight:166750.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate manganese(2+)

Cross database links:

RefSeq: NP_014934.1   
Entrez Gene ID: 854466   
Pfam: PF00122    PF00702   
KEGG: sce:YOR291W   

Gene Ontology

GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0015662 F:ATPase activity, coupled to transmembrane m...
GO:0046872 F:metal ion binding
GO:0006754 P:ATP biosynthetic process
GO:0006812 P:cation transport

References (3)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.”  Dujon B.et.al.   9169874
[2] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[3] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
FASTA formatted sequence 
1:	MDIPSSNQIQ HGQRSERNRR MPRASFSSTA TTSTAATLTS AMVLDQNNSE PYAGATFEAV 
61:	PSSIVSFHHP HSFQSSNLPS PHSSGNLEQR GRRLTESEPL VLSSAEQSRS SSRNPSHFRF 
121:	FTQEQISNAE GASTLENTDY DMAWDATPAY EQDRIYGTGL SSRRSSIRSF SRASSLSNAK 
181:	SYGSFSKRGR SGSRAPQRLG ENSDTGFVYH SATHSSSSLS RYTTRERIPI ELESQTDEIL 
241:	EDESSTHSLE SSDSRRSASE NNRGSFSGHD DVHNQHSEYL KPDYHEKFYP QYAPNLHYQR 
301:	FYIAEEDLVI GIAAYQTSKF WYIIYNLCCF LTFGLVYLLT RWLPHLKVKL YGVKVPLAKA 
361:	EWVVIENEFG EFVIQPIDRQ WYNRPLSTVL PFENYPNPSY EPNDINLSHH HANEINPNVP 
421:	ILITFEYRYI KFIYSPLDDL FKTNNNWIDP DWVDLSTVSN GLTKGVQEDR ELAFGKNQIN 
481:	LRMKTTSEIL FNEVLHPFYV FQVFSIILWG IDEYYYYAAC IFLISVLSIF DSLNEQKKVS 
541:	RNLAEMSHFH CDVRVLRDKF WTTISSSELV PGDIYEVSDP NITILPCDSI LLSSDCIVNE 
601:	SMLTGESVPV SKFPATEETM YQLCDDFQST QISSFVSKSF LYNGTNIIRA RIAPGQTAAL 
661:	AMVVRTGFST TKGSLVRSMV FPKPTGFKFY RDSFKYIGFM SLIAIFGFCV SCVQFIKLGL 
721:	DKKTMILRAL DIITIVVPPA LPATLTIGTN FALSRLKEKG IFCISPTRLN ISGKIDVMCF 
781:	DKTGTLTEDG LDVLGVQISE PNGVRGQKFG ELLSDIRQVF PKFSLNDCSS PLDFKSRNFF 
841:	MSLLTCHSLR SVDGNLLGDP LDFKMFQFTG WSFEEDFQKR AFHSLYEGRH EDDVFPENSE 
901:	IIPAVVHPDS NNRENTFTDN DPHNFLGVVR SFEFLSELRR MSVIVKTNND DVYWSFTKGA 
961:	PEVISEICNK STLPADFEEV LRCYTHNGYR VIACAGKTLP KRTWLYSQKV SREEVESNLE 
1021:	FLGFIIFQNK LKKETSETLK SLQDANIRTI MCTGDNILTA ISVGREAGLI QCSRVYVPSI 
1081:	NDTPLHGEPV IVWRDVNEPD KILDTKTLKP VKLGNNSVES LRECNYTLAV SGDVFRLLFR 
1141:	DENEIPEEYL NEILLNSSIY ARMSPDEKHE LMIQLQKLDY TVGFCGDGAN DCGALKAADV 
1201:	GISLSEAEAS VAAPFTSKIF NISCVLDVIR EGRAALVTSF ACFQYMSLYS AIQFITITIL 
1261:	YSRGSNLGDF QFLYIDLLLI VPIAICMSWS KSYEKIDKKR PSANLVSPKI LVPLLISVFL 
1321:	VFLFQFIPWI IVQKMSWYIK PIVGGDDAVQ SSDNTVLFFV SNFQYILTAI VLSVGPPYRE 
1381:	PMSKNFEFIV DITVSIGASL LLMTLDTESY LGKMLQLTPI SNSFTMFIIV WVILNYYAQL 
1441:	YIPPSIKGWL KKKKSSKKYK LLIQEEMKLK EV