3.A.3.2.47
Putative Ca2+ P-type ATPase, TMEM94, of 1356 aas and 10 TMSs in the usual 2 + 2 + 6 TMS arrangement. This protein is very distantly related to all other members of the 3.A.3.2 family within the P-type ATPase superfamily, and therefore may have a different or unique function (Zhang et al. 2018). It may functioin with NPC2 of 123 aas and 1 N-terminal TMS. ERMA (TMEM94) transports Mg2+ uptake in the endoplasmic reticulum (Vishnu et al. 2024). The ER is a major Mg2+ compartment refilled by TMEM94. ERMA uniquely combines a P-type ATPase domain and a GMN motif for ERMg2+ uptake. A tyrosine residue is crucial for Mg2+ binding, and activity is a mechanism conserved in both prokaryotic (MgtB and MgtA) and eukaryotic Mg2+ ATPases. Cardiac dysfunction by haploinsufficiency, abnormal Ca2+ cycling in mouse Erma+/- cardiomyocytes, and ERMA mRNA silencing in human iPSC-cardiomyocytes collectively define ERMA as an essential component of ERMg2+ uptake in eukaryotes (Vishnu et al. 2024).
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| Accession Number: | Q12767 |
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| Protein Name: | Transmembrane protein 94 |
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| Length: | 1356 |
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| Molecular Weight: | 151151.00 |
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| Species: | Homo sapiens (Human) [9606] |
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| Number of TMSs: | 8 |
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| Location1 / Topology2 / Orientation3: |
Membrane1 / Multi-pass membrane protein2 |
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| Substrate |
magnesium(2+) |
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1: MDLKEKHLGE PPSALGLSTR KALSVLKEQL EAVLEGHLRE RKKCLTWKEV WRSSFLHHSN
61: RCSCFHWPGA SLMLLAVLLL LGCCGGQPAG SRGVGLVNAS ALFLLLLLNL VLIGRQDRLK
121: RREVERRLRG IIDQIQDALR DGREIQWPSA MYPDLHMPFA PSWSLHWAYR DGHLVNLPVS
181: LLVEGDIIAL RPGQESFASL RGIKDDEHIV LEPGDLFPPF SPPPSPRGEV ERGPQSPQQH
241: RLFRVLETPV IDNIRWCLDM ALSRPVTALD NERFTVQSVM LHYAVPVVLA GFLITNALRF
301: IFSAPGVTSW QYTLLQLQVN GVLPILPLLF PVLWVLATAC GEARVLAQMS KASPSSLLAK
361: FSEDTLSSYT EAVSSQEMLR CIWGHFLRVL GGTSPTLSHS SSLLHSLGSV TVLCCVDKQG
421: ILSWPNPSPE TVLFFSGKVE PPHSSHEDLT DGLSTRSFCH PEPHERDALL AGSLNNTLHL
481: SNEQERGDWP GEAPKPPEPY SHHKAHGRSK HPSGSNVSFS RDTEGGEEEP SKTQPGMESD
541: PYEAEDFVCD YHLEMLSLSQ DQQNPSCIQF DDSNWQLHLT SLKPLGLNVL LNLCDASVTE
601: RLCRFSDHLC NIALQESHSA VLPVHVPWGL CELARLIGFT PGAKELFKQE NHLALYRLPS
661: AETMKETSLG RLSCVTKRRP PLSHMISLFI KDTTTSTEQM LSHGTADVVL EACTDFWDGA
721: DIYPLSGSDR KKVLDFYQRA CLSGYCSAFA YKPMNCALSS QLNGKCIELV QVPGQSSIFT
781: MCELPSTIPI KQNARRSSWS SDEGIGEVLE KEDCMQALSG QIFMGMVSSQ YQARLDIVRL
841: IDGLVNACIR FVYFSLEDEL KSKVFAEKMG LETGWNCHIS LTPNGDMPGS EIPPSSPSHA
901: GSLHDDLNQV SRDDAEGLLL MEEEGHSDLI SFQPTDSDIP SFLEDSNRAK LPRGIHQVRP
961: HLQNIDNVPL LVPLFTDCTP ETMCEMIKIM QEYGEVTCCL GSSANLRNSC LFLQSDISIA
1021: LDPLYPSRCS WETFGYATSI SMAQASDGLS PLQLSGQLNS LPCSLTFRQE ETISIIRLIE
1081: QARHATYGIR KCFLFLLQCQ LTLVVIQFLS CLVQLPPLLS TTDILWLSCF CYPLLSISLL
1141: GKPPHSSIMS MATGKNLQSI PKKTQHYFLL CFLLKFSLTI SSCLICFGFT LQSFCDSSRD
1201: RNLTNCSSVM LPSNDDRAPA WFEDFANGLL SAQKLTAALI VLHTVFISIT HVHRTKPLWR
1261: KSPLTNLWWA VTVPVVLLGQ VVQTAVDLQL WTHRDSHVHF GLEDVPLLTW LLGCLSLVLV
1321: VVTNEIVKLH EIRVRVRYQK RQKLQFETKL GMNSPF