3.A.3.3.10
Plamsa membrane proton-pumping ATPase, Pma1, of 1003 aas and 10 putative TMSs in a 2 + 2 + 6 TMS arrangement. Leptosphaeria maculans, lacking this enzyme, displays a total loss of pathogenicity towards its host plant (Brassica napus). The mutant is unable to germinate on the host leaf surface and is
thus blocked at the pre-penetration stage. Reduction in Pma1 activity may disturb the
electrochemical transmembrane gradient, thus leading to conidia defective in turgor pressure
generation on the leaf surface. L. maculans possesses a second plasma
membrane H+-ATPase-encoding gene, termed pma2 (Remy et al. 2008).
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| Accession Number: | B3LDT4 |
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| Protein Name: | Plasma membrane ATPase |
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| Length: | 1003 |
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| Molecular Weight: | 110392.00 |
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| Species: | Leptosphaeria maculans (Blackleg fungus) [5022] |
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| Number of TMSs: | 12 |
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| Substrate |
hydron |
|---|
1: MAPNDDVERP RASSAAERQK SIVSMTEFEG MDEYTALQKY ILFYRDARTN PHAEKTIKKK
61: AWWQFWKSGS ATAAVPAADP GAVPDEFLNT ELRTGLTTTE VESRRKRYGF NEISSEKTNL
121: LKQFIGYFTG PILYVMELAA LLAAGLQDWI DFGVICGILL LNAIVGWYQE KQAADVVASL
181: KGDIAMKATV VRDNQQQTIL ARELVPGDIV VVEEGQTVPG DVRLICGYDH PEDFELYMKL
241: KAEDKFHDGD PEDEKDDEID EEKFDEENPI TQGHPLVACD QSSITGESLA VDKYMGEVAY
301: YTTGCKRGKA YGIVITTAKH SFVGRTASLV QGAQDQGHFK AIMNSIGTAL LVLVMFFILL
361: AWIGGFFRNI PISKAREGTD KSVTLLHYAL IMFIVGVPVG LPVVTTTTLA VGAAYLAEQK
421: AIVQKLTAIE SLAGVDVLCS DKTGTLTANQ LSLREPYVAE GQDVNWMMAV AALASSHNLK
481: SLDPIDKVTI LTIRRYPKAR EILNMGWKTE KFTPFDPVSK RITAVCHMGG DKYVCAKGAP
541: KAIVNLANCD EITATLYKEK AAEFARRGFR SLGVAYQKND GDWILLGLLS MFDPPREDTA
601: QTIVEAQQLG VPVKMLTGDA IAIAKETCKM LALGTKVYNS SKLIHGGLTG TTQHDLVERA
661: DGFAEVFPEH KYQVVEMLQQ RGHLTAMTGD GVNDAPSLKK SDCGIAVEGS TEAAQAAADI
721: VFLAPGLSTI VLAIKTARQI FQRMKAYIQY RIALCLHLEV YLVTSMIILN ETIRAELIVF
781: LALFADLATV AVAYDNAHSE PRPVEWQLPK IWLISVVLGL LLALGTWVIR GALFLPNGGI
841: IQNFGAIQPI LFLEVALTEN WLIFVTRGGK TFPSFQLIIA ILGVDALATI FTVFGWMSGE
901: PYQTNPPTNN TKFRDNGWVD IVTVVVIWAY SIGVTIIIAI VYYLLNRIAW LDTLGRKDRS
961: RKNPAIENMI AALSKLSLEH GTDKNGTTRY VLAPRAVEEE DDE