3.A.3.5.42
Copper (Cu+) exporting ATPase of 1254 aas and 8 TMSs, CrpA. While the A. flavus protein has not been characterized at the date of this entry, the A. fumigatus ortholog has been (Werner et al. 2023). Cellular responses used by macrophages to counteract fungal infection is the accumulation of high phagolysosomal Cu levels to destroy ingested pathogens. A. fumigatus responds by activating high expression levels of crpA, which encodes a CrpA that actively transports excess Cu+ from the cytoplasm to the extracellular environment (Werner et al. 2023). Deletion of CrpA fungal-unique amino acids 1-211, containing two N-terminal Cu-binding sites, moderately increased Cu-sensitivity but did not affect expression or localization to the ER and cell surface. Replacement of CrpA fungal-unique amino acids 542-556, consisting of an intracellular loop between the second and third TMSs resulted in ER retention of the protein and strongly increased Cu-sensitivity (Werner et al. 2023).
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| Accession Number: | QRD90192 |
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| Protein Name: | QRD90192.1 putative copper resistance-associated P-type ATPase [Aspergillus flavus] |
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| Length: | 1255 |
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| Molecular Weight: | |
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| Species: | Aspergillus flavus [5059] |
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| Number of TMSs: | 9 |
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| Substrate |
copper(1+) |
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1: MSPISDHASR NPADQDSRHF KSTFFVSNVH CSSCIAYITE VLSELSGVFG VDVTILTHEV
61: RVSHAAETSP STLAKALIQA AFEVHHVTTC DDKGMVVSDI STTSWLSKDS VPFATQQASY
121: SSSTTQNRHL ANCDACRKEE LQGISEVRNL SETTPTRLRD EKNSQLFGVS DEVSDIASLA
181: PRKSAENAKS LQPLGDNGKF NARISIEGMS CASCVNTVTN EVQQLNFVME ITVNLLSNSA
241: TVLYTGPQSN IEKVIEHIED IGFEASLDEV EQIGVTPPQN RTSSSYVAEI AIGGMTCGSC
301: AGSVTRGLEE LPFVTKVSVN LLSHSGTVEF DGHDHIDTIV EKIEDLGYDA SVTSVLPQAG
361: EGQDDGKIER RTVAIRVEGM FCHHCPEKVL NSLKELSNVE IDGTLSVKNP IVKVTYTPRP
421: PSLTIRAILQ RIDDVHDAFN ATVYHPPSIE DRSRAIQLHE RRRLLSRFLF VLIVAIPTFL
481: IGIVFMSLVS SDNQIRKYLE QPMWAGSVSR MEWSLFIMTT PVMFYGTDLF HVRAAKEIYS
541: LWRPGSRVPI LRRFYRFGSM NLLISAGTTV AYVSSLAVLI IDAVVGASSM SNSTTYFDSV
601: VFLTLFILAG RFIEAYSKAR TGDAVASLGK LKPSEALLIV DNRAAEQNDV GASSGEDEIH
661: RINIELLDVG DIVSIPHGAS PPADGVVVDT NTYQFDESSL TGESRPVKKS AGDPVYTGSV
721: NVGQPVKIKV SAVGGASMLD QIIAVVREGQ SRRAPLERVA DLLTSHFVPI ITLIAVLTFI
781: IWLALGASGA LPDDYLDVAH GGWAFWSLEF AIAVFVVACP CGLALAAPTA LFVGGGLAAR
841: HGILVKGGGE AFQEASRLDA IVFDKTGTLT EGGSLTVSDH ELLITDSEEL QVVWSLARKL
901: EESSNHPIAH AVSAFCSGKE SASILSSDIE ERPGQGMKGR FTVSLVDKTD QSKRTTQYEA
961: AIGNQRLLQS LTSPNFDDSQ LSKLLSTYQT EGKSTAILSL RKVGSGSSND PYFTPAIIFA
1021: ISDSIRPDAA QIISQLQKRK VDVYMCTGDN QTTAHAVADM VGIPRSNVMA NIMPAGKAEF
1081: VRQVQDGVYP PRTDSEAESQ RNEQGTRSIV AFVGDGVNDS PALAAADVSI AMASGSDVAM
1141: NSASFILLNS ELNTILQLVV LSRRVFNRVK MNFGWAVVYN LCLVPVAAGV FYPIVNGHEM
1201: KIIGGETVMV DKHWRLSPVW AALAMALSSI SVVCSSLALG IEKKTIKRLL GLDK