3.A.3.8.15
Phospholipid transporting ATPase, Tat1 of 1139 aas. Transports phosphatidylserine from the
outer to the inner leaflet of the plasma membrane, thereby maintaining
the enrichment of this phospholipid in the inner leaflet. Ectopic
exposure of phosphatidylserine on the cell surface may result in removal
of living cells by neighboring phagocytes in an apoptotic process (Darland-Ransom et al. 2008). Tat1 regulates lysosome biogenesis and endocytosis as well as yolk uptake in oocytes. It is required at multiple steps
of the endolysosomal pathway, at least in part by ensuring proper trafficking of cell-specific
effector proteins (Ruaud et al. 2009). TAT-1 and its chaperone, the
Cdc50 family protein CHAT-1, maintain membrane phosphatidylserine (PS)
asymmetry, which is required for membrane tubulation during endocytic
sorting and recycling. Loss of tat-1 and chat-1 disrupts endocytic
sorting, leading to defects in both cargo recycling and degradation. Chen et al. 2019 identified the C. elegans aspartyl aminopeptidase DNPP-1,
loss of which suppresses the sorting and recycling defects in tat-1 mutants without reversing the PS asymmetry defect.
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| Accession Number: | Q9U280 |
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| Protein Name: | Phospholipid-transporting ATPase tat-1 |
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| Length: | 1139 |
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| Molecular Weight: | 128411.00 |
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| Species: | Caenorhabditis elegans [6239] |
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| Number of TMSs: | 7 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
phospholipid, phosphatidyl-L-serine |
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1: MPTEARDNNR HIHLGKVRDP HHQHAQRFCS NRISTCKYNG FSFLPRFLYE QFRRYNNIFF
61: LAIALLQQIP DVSPTGRYTT AVPFLIILSV SALKEIFEDV KRRRSDNKVN AFSVEILVDG
121: HWIEKQWKDV SVGDFIRIDN DSLFPADLLL LASSEQQGMA YIETSNLDGE TNLKIKQALD
181: ITSTMTSPEK LSQFESEITC EPPSRHVNEF NGNIEINGVA RHFGIDQLLL RGARLKNTAW
241: IFGAVIYTGH DSKLLMNSKR APLKSGTIDV QTNYRIIFLF FVLVALALIS ATGSEIWRGN
301: NIPQAWYLSF LEHDPKGSFL WGVLTFFILY NNLIPISLQV TLEVVRFFQA IYINNDIEMY
361: DVNSDSCAIA RTSNLNEELG QVKFIMSDKT GTLTRNVMKF KRLSIGSRNY GNNEDDEFAD
421: ASLIEDYRQG DEHSTSILEV LKMMAVCHTV VPENKDGQLI YQSSSPDEAA LVRGAASQSV
481: SFHTRQPQKV ICNVFGEDET IEILDVIDFT SDRKRMSVIV RDGAGGDIKL YTKGADTVIF
541: ERLEHGKEQE EAVEYCTEHL EDYASFGYRT LCFSMRHLTE QEYSQWAPEY KKAILAIDNR
601: AKLLADAAEK LERNMILVGA TAIEDKLQEW VPETIQALMA ADIRVWMLTG DKRETAINIA
661: HSCALCHTNT ELLIVDKTTY EETYQKLEQF VARAIELEKQ EKGFAMVIDG KSLLHALTGE
721: ARKHFGDLAL RCHAVVCCRM SPMQKAEVVE MVRKLAKHVV LAIGDGANDV AMIQAANVGV
781: GISGEEGLQA ASASDYAIPR FHFLRRLLLV HGAWNHDRSV KVILYSFYKN ICLYIIELWF
841: AMFSAWSGQT IFERWTIGMF NVIFTAWPPV VLGLFDHPVP AEQIMKYPAL YASFQNRAFS
901: IGNFSLWIGL AIVHSLSLFF LTYATMEHQV VWDNGLTGGW LMLGNCAYTF VVATVCFKAL
961: LECDSWTWPV VVACIGSIGL WIVFVIVYSL VFPHIGGIGA DMAGMAAIMM SSYTFWLALL
1021: FIPLATLLWD LVIKSLFTIA MPTPRELAVM YNKRTTSFNG FERLASYSSN VLENMRLLTS
1081: SLRGSTTGST RSRTASEASL ALAEQTRYGF AFSQDESSAV AQTELIRNVD STREKPTGR