3.A.3.8.19
The Leishmania miltefosine transporter (LMT) is a plasma membrane
P4-ATPase that catalyses translocation into the parasite of the
leishmanicidal drug, miltefosine as well as phosphatidylcholine and
phosphatidylethanolamine analogues. Five highly-conserved amino acids in
the cytosolic N-terminal tail (Asn58, Ile60, Lys64, Tyr65 and Phe70)
and two (Pro72 and Phe79) in the first TMS were examined, and several of
these were important for activity (Perandrés-López et al. 2018). The beta subunit of this system has TC# 8.A.27.1.3.
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| Accession Number: | AHM92900.1 |
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| Protein Name: | AHM92900.1 miltefosine transporter [Leishmania amazonensis] |
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| Length: | 1105 |
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| Molecular Weight: | |
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| Species: | Leishmania amazonensis [5659] |
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| Number of TMSs: | 9 |
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| Substrate |
1,2-diacyl-sn-glycero-3-phosphocholine(1+), phosphatidylethanolamine, miltefosine |
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1: MPNQPACWRK CLPTRILPGK LSKSFCCFSA EADVDEDDEV IVYFNNPEAN AQFKYPSNFI
61: RTSKYSLISF LPLSLLYEFR KVSNLYFLIN VIFSLIPGVS PLNPATSIAP LSFVLLVAII
121: KEAVEDIKRH RADNRANSVL TQVVRKGKLV SVHSKDIHPG DVVRIKNSEE VHADVVMLSS
181: SLEEGQAFID TCNLDGESNL KPRRALEVTW GLCKIETIMN TTAVLHTSKP DPGLLSWTGL
241: LEINGEEHAL SLDQFLYRGC VLRNTDWAWG MIAYAGVDTK LFRNLKPKPP KSSNLDRKLN
301: YFIIAILIFQ NIMLFILASM AVWWNNKYKE TPYLRFFINS RQNITLWGYR YLSYFILLSY
361: CVPISLFVTI EVCKVIQAQW MRVDCLMMEY MNNRWRHCQP NTSNLNEQLG MVRFIFSDKT
421: GTLTENVMKF KQGDALGIPI EADSLDKCIV QLRKETESKR LGPLQEYFLA LALCNTVQPF
481: KDDTDGLSVI YEGSSPDEVA LVETAAAVGY RLINRTTKSI TLLLQNDTRK VYNILATLEF
541: TPDRKMMSII VEDSDTKQIM LYNKGADSFI RPQLSRAPDV QEHIESVDIP LTEMSSSGLR
601: TLLVCAKDIT RRQFDLWYEK FVEVGKSLHN RSSKIDKVCL EMEQDMRLVG ATAIEDKLQD
661: EVPETLSFFL NAGVIIWMLT GDKRETAVTI AATSTLCDPR NDFIDHVDIG HLNPSDPKAI
721: ERVGRDLDVV EQHIALKGTH KERRCTLVID GPALNISMEH YFNQFLRISH QLNSAVCCRL
781: TPIQKASVVR MFQKSTGKTA LAIGDGANDV SMIREGRVGV GIIGVEGAHA ALAADYAIPR
841: FKHLRRLCAV HGRYSLFRNA SCILVSFHKN ITVSVVQFIF AFYVGFSGLT LFDGWMLTFY
901: NVLMTSVPPF FMGIFDKDLP EEALLERPKL YTPLSHGEYF NVATLLRWFA ESLITALILF
961: YAAYPTLVHQ DGSHQRYTGA ETGTLVFSGL ILVIQTRFAL QIRYWQWLQV FGMVMALSFF
1021: LLLFLVYSAI PSIFSDTNFY YQAFDLMSTA KYWFFLLLYV GIELVAVLGF IVFQKSLFPT
1081: LRDVAERQHA LQKSGRRRRS LDSA