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Glucose porter (PtsG; GlcA; Umg) (transports D-glucose and α-methyl-D-glucopyranoside).  The IIC domain has been crystallized, and x-ray data to 4.5 Å resolution have been described (Zurbriggen et al. 2010).  The system has been manipulated to engineer increased production of aromatic metabolites (Carmona et al. 2015, Vargas-Tah et al. 2015). The presence or absence of D-glucose reflects the transporter before and after release of the transported glucose into the cytoplasm. The transition associated with substrate release appears to require a subtle structural rearrangement in the region that includes hairpin 1 (Kalbermatter et al. 2017).  Mlc (for makes large colonies) represses transcription of the genes encoding enzyme I, HPr, EIIBCGlc and EIIABCDMan in defined media that lack PTS substrates. When glucose is present, the unphosphorylated form of EIIBCGlc sequesters Mlc to the cell membrane, preventing its interaction with DNA (Plumbridge 2002, Joyet et al. 2013). The Vibrio Mlc functions similarly (Pickering et al. 2014). A small (43 aa) protein, SgrT, acts in tandem with a well-characterized small RNA during metabolic stress, due to the accumulation of cytoplasmic sugar-Ps to help bacterial cells maintain balanced metabolism and continue growing. SgrT acts on the glucose transport system, inhibiting its activity under stress conditions in order to allow cells to utilize alternative carbon sources (Lloyd et al. 2017). ptsG mRNA localization to the inner membrane, coupled with the membrane insertion of nascent peptide, mediates Hfq/SgrS-dependent ptsG mRNA destabilization, presumably by reducing second rounds of translation (Kawamoto et al. 2005). SgrT is a small protein of 43 aas that allosterically inhibits IICBGlc. while SgrS is a small RNA coompementary to ptsG mRNA that influences its expression.  The sgrST operon is regulated by SgrR, a glucose-6-P-dependent transcriptional activator (Jeckelmann and Erni 2020).  

Accession Number:P69783
Protein Name:Glucose-specific phosphotransferase enzyme IIA component PTGA aka CRR aka GSR aka IEX aka TGS aka TRED aka B2417
Molecular Weight:18251.00
Species:Escherichia coli [83333]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate D-glucopyranose

Cross database links:

DIP: DIP-31863N
RefSeq: AP_003011.1    NP_416912.1   
Entrez Gene ID: 946880   
Pfam: PF00358   
BioCyc: EcoCyc:CRR-MONOMER    ECOL168927:B2417-MONOMER   
KEGG: ecj:JW2410    eco:b2417   

Gene Ontology

GO:0005829 C:cytosol
GO:0016020 C:membrane
GO:0016301 F:kinase activity
GO:0046872 F:metal ion binding
GO:0005515 F:protein binding
GO:0005351 F:sugar:hydrogen symporter activity
GO:0009401 P:phosphoenolpyruvate-dependent sugar phospho...

References (16)

[1] “The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription.”  de Reuse   2457575
[2] “Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes.”  Saffen   2960675
[3] “Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12.”  Yang   8764513
[4] “Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates.”  Hall   1630305
[5] “Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features.”  Yamamoto   9205837
[6] “The complete genome sequence of Escherichia coli K-12.”  Blattner   9278503
[7] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi   16738553
[8] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link   9298646
[9] “Protein identification with N and C-terminal sequence tags in proteome projects.”  Wilkins   9600841
[10] “Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc.”  Worthylake   1961703
[11] “Structure of the regulatory complex of Escherichia coli IIIGlc with glycerol kinase.”  Hurley   8430315
[12] “Cation-promoted association of a regulatory and target protein is controlled by protein phosphorylation.”  Feese   8170944
[13] “Structural studies of the Escherichia coli signal transducing protein IIAGlc: implications for target recognition.”  Feese   9405042
[14] “1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques.”  Pelton   1911770
[15] “Secondary structure of the phosphocarrier protein IIIGlc, a signal-transducing protein from Escherichia coli, determined by heteronuclear three-dimensional NMR spectroscopy.”  Pelton   2014267
[16] “Structural comparison of phosphorylated and unphosphorylated forms of IIIGlc, a signal-transducing protein from Escherichia coli, using three-dimensional NMR techniques.”  Pelton   1606145
1F3G   1F3Z   1GGR   1GLA   1GLB   1GLC   1GLD   1GLE   1O2F   2F3G   [...more]

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