4.A.6.1.17 D-glucosaminate group translocating uptake porter, DgaABCD (IIA-141 aas, IIB-161 aas, IIC-249 aas, and IID-285 aas, respectively) (Miller et al. 2013). Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium)
uses d-glucosaminate (2-amino-2-deoxy-d-gluconic acid) as a carbon and
nitrogen source via DgaABCD (d-glucosaminate PTS permease components EIIA, EIIB, EIIC, and EIID). Two other genes in the
dga operon (dgaE and dgaF) are required for wild-type growth with d-glucosaminate. Transcription of dgaABCDEF is
dependent on RpoN (σ54) and an RpoN-dependent activator gene, dgaR. Introduction of a plasmid bearing dgaABCDEF under the
control of the lac promoter into E. coli strains allowed them to grow on minimal
medium containing d-glucosaminate. d-Glucosaminate is transported and
phosphorylated at the C-6 position by DgaABCD. DgaE converts the
resulting d-glucosaminate-6-phosphate to 2-keto-3-deoxygluconate
6-phosphate (KDGP), which is subsequently cleaved by the aldolase DgaF
to form glyceraldehyde-3-phosphate and pyruvate. DgaF catalyzes the same
reaction as that catalyzed by Eda, a KDGP aldolase in the
Entner-Doudoroff pathway, and the two enzymes can substitute for each
other in their respective pathways. Orthologs of the dga genes are
largely restricted to certain enteric bacteria and a few Firmicutes (Miller et al. 2013).
|
Accession Number: | D0ZLR5 |
Protein Name: | Putative phosphotransferase system enzyme IIC |
Length: | 249 |
Molecular Weight: | 26785.00 |
Species: | Salmonella typhimurium (strain 14028s / SGSC 2262) [588858] |
Number of TMSs: | 6 |
Substrate |
2-amino-2-deoxy-D-gluconic acid |
---|
1: MDTLLFASLM GLYYWFARLR LGYTFSAMLL QPVVIAVFVG LLLGNMQTAM IIGAGMQLVY
61: LGVTSTPGGN VPSDPALAAC ISIPIAVKAG MDPNLAIALA IPFGVIGVFL DQLRRTLNAA
121: WVHMADKHAE TANMAGIMRC AFLYPALLGL VLRFPVVFAA NYFGQDVVES FLKLMPHWLT
181: HSFEIMGGIL PALGFAITIM VIGKKSLLPW FIGGFFAVLY LKVDIMAMAI FGTCVAFLIK
241: GLAKNEGAA