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4.D.1.1.3
PgaC or YcdQ of 441 aas with a 5 (2 (N-terminal) + 3 (C-terminal)) TMS topology. TC Blast retrieves 4.D.3.1.5 and 4.D.2.1.9 with scores of e-13 and e-8, respectively, for the hydrophilic catalytic domains, demonstrating homology.  This enzyme is an N-acetylglucosaminyltransferase that catalyzes the polymerization of UDP-N-acetylglucosamine to produce the linear homopolymer, poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide (Cerca and Jefferson 2008).  May function with PgaD, a 137 aa, 2 TMS protein (P69432). The polysaccharide is partially deacetylated by PgaB (P75906) in preparation for export across the outer membrane by PgaA (TC# 1.B.55.1.1).

Accession Number:P75905
Protein Name:YcdQ aka B1022
Length:441
Molecular Weight:50766.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate UDP-N-acetyl-alpha-D-glucosamine, N-acetyl-beta-D-glucosamine

Cross database links:

DIP: DIP-11512N DIP-11512N
RefSeq: AP_001651.1    NP_415541.1   
Entrez Gene ID: 945606   
Pfam: PF00535   
BioCyc: EcoCyc:G6529-MONOMER    ECOL168927:B1022-MONOMER    MetaCyc:G6529-MONOMER   
KEGG: ecj:JW1007    eco:b1022    eco:b1022   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008375 F:acetylglucosaminyltransferase activity
GO:0042710 P:biofilm formation

References (11)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation.”  Wang X.et.al.   15090514
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[7] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[8] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[9] “The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation.”  Wang X.et.al.   15090514
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[11] “Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine.”  Itoh Y.et.al.   18359807

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MINRIVSFFI LCLVLCIPLC VAYFHSGELM MRFVFFWPFF MSIMWIVGGV YFWVYRERHW 
61:	PWGENAPAPQ LKDNPSISII IPCFNEEKNV EETIHAALAQ RYENIEVIAV NDGSTDKTRA 
121:	ILDRMAAQIP HLRVIHLAQN QGKAIALKTG AAAAKSEYLV CIDGDALLDR DAAAYIVEPM 
181:	LYNPRVGAVT GNPRIRTRST LVGKIQVGEY SSIIGLIKRT QRIYGNVFTV SGVIAAFRRS 
241:	ALAEVGYWSD DMITEDIDIS WKLQLNQWTI FYEPRALCWI LMPETLKGLW KQRLRWAQGG 
301:	AEVFLKNMTR LWRKENFRMW PLFFEYCLTT IWAFTCLVGF IIYAVQLAGV PLNIELTHIA 
361:	ATHTAGILLC TLCLLQFIVS LMIENRYEHN LTSSLFWIIW FPVIFWMLSL ATTLVSFTRV 
421:	MLMPKKQRAR WVSPDRGILR G