4.D.3.1.14
Cellulose synthase, CesA7, of 1042 aas and 8 TMSs in a 2 +3 + 3 TMS arrangement where the first two TMSs are in the first half of the protein while the last 6 are C-terminal. The cryo-EM structure of the homotrimeric CesA7 from Gossypium hirsutum has been reported at 3.5 Å resolution (Zhang et al. 2021). The GhCesA7 homotrimer shows a C3 symmetrical assembly. Each protomer contains seven transmembrane helices (TMSs) which form a channel potentially facilitating the release of newly synthesized glucans. The cytoplasmic glycosyltransferase domain (GT domain) of GhCesA7 protrudes from the membrane, and its catalytic pocket is directed towards the TM pore. The homotrimer GhCesA7 is stabilized by transmembrane helix 7 (TMS 7) and the plant conserved region (PCR) domains. It represents the building block of CSCs and facilitates microfibril formation. This structure provides insight into how eukaryotic cellulose synthase assembles (Zhang et al. 2021).
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| Accession Number: | L7NUA2 |
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| Protein Name: | Cellulose synthase |
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| Length: | 1042 |
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| Molecular Weight: | 117991.00 |
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| Species: | Gossypium hirsutum (Upland cotton) [3635] |
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| Number of TMSs: | 8 |
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| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
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| Substrate |
(1->4)-beta-D-glucan |
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1: MEASAGLVAG SHNRNELVVI HGHEEPKPLK NLDGQVCEIC GDEIGVTVDG DLFVACNECG
61: FPVCRPCYEY ERREGTQQCP QCKTRYKRLK GSPRVEGDED EEDVDDIEHE FNIDDEQNKH
121: RNVVESILHG KMSYGRGPED DETPQIPVIT GVRSRPVSGE FPIAGALAYG EHMPNASLHK
181: RVHPYPMSET EGAERWDDKK EGGWKERMDD WKMQQGNLGP EADDAYDDMS MLDEARQPLS
241: RKVPIASSKI NPYRMVIVAR LLILAFFLRY RILNPVHDAI GLWLTSVICE IWFAFSWILD
301: QFPKWFPIDR ETYLDRLSLR YEREGEPNML APVDIFVSTV DPMKEPPLVT ANTVLSILAM
361: DYPVDKISCY ISDDGASMLT FESLSETAEF ARKWVPFCKK FAIEPRAPEM YFTLKVDYLK
421: DKVQPTFVKE RRAMKREYEE FKVRINALVA KAQKVPPEGW IMQDGTPWPG NNTKDHPGMI
481: QVFLGQSGGH DTEGNELPRL VYVSREKRPG FLHHKKAGAM NALVRVSGVL TNAPFMLNLD
541: CDHYINNSKA AREAMCFLMD PQIGRKVCYV QFPQRFDGID RHDRYANRNT VFFDINMKGL
601: DGIQGPVYVG TGCVFRRQAL YGYEPPKGPK RPKMVSCGCC PCFGRRKKDK KYPKNGGNEN
661: GPSLEAVEDD KELLMSQMNF EKKFGQSAIF VTSTLMDQGG VPPSSSPAAL LKEAIHVISC
721: GYEDKTEWGS ELGWIYGSIT EDILTGFKMH CRGWRSIYCM PKLPAFKGSA PINLSDRLNQ
781: VLRWALGSVE IFFSRHCPAW YGLKGAKLRW LERFAYVNTT IYPFTSLPLL AYCTLPAICL
841: LTDKFIMPPI STFASLFFIA LFLSIFATGI LELRWSGVSI EEWWRNEQFW VIGGISAHLF
901: AVVQGLLKVL AGIDTNFTVT SKTTDDEEFG ELYTFKWTTL LIPPTTVLII NLVGVVAGIS
961: DAINNGYQSW GPLFGKLFFS FWVIVHLYPF LKGLMGRQNR TPTIVVIWSV LLASIFSLLW
1021: VRIDPFVLKT KGPDTTQCGI NC