4.D.3.1.8
Cellulose synthase-like protein of 952 aas and about 7 TMSs, 3 (or 4) N-terminal and 6 C-terminal. May catalyze both beta-1,3 and beta-1,4
glycosidic linkages in beta-D-glucan. Essential for
(1,3;1,4)-beta-D-glucans synthesis in grasses and cereals (Poaceae). The
mixed-linked glucans (which are not present in walls of dicotyledons or
most other monocotyledonous plants) are particularly important
constituents of the walls of the starchy endosperm and aleurone cells of
cereal grains such as oats, wheat, rice and barley. They can account
for up to 70% by weight of the wall. A single amino acid within the predicted
transmembrane pore domain of CslF6 controls (1-3,1-4)-beta-glucan structure. The membrane pore architecture and the
translocation of the growing polysaccharide across the membrane control how the acceptor glucan is
coordinated at the active site and thus the proportion of beta1-3 and beta1-4 bonds within the
polysaccharide (Jobling 2015). Residues involved in catalyses and flexibility have been identified (Dimitroff et al. 2016). These influence the ratio of 1,3 to 1,4 linkages (Purushotham et al. 2022).
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| Accession Number: | Q84UP7 |
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| Protein Name: | Probable mixed-linked glucan synthase 6 |
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| Length: | 952 |
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| Molecular Weight: | 105152.00 |
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| Species: | Oryza sativa subsp. japonica (Rice) [39947] |
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| Number of TMSs: | 8 |
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| Location1 / Topology2 / Orientation3: |
Golgi apparatus membrane1 / Multi-pass membrane protein2 |
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| Substrate |
glycosyl group |
|---|
1: MAPAVAGGGG RRNNEGVNGN AAAPACVCGF PVCACAGAAA VASAASSADM DIVAAGQIGA
61: VNDESWVAVD LSDSDDAPAA GDVQGALDDR PVFRTEKIKG VLLHPYRVLI FVRLIAFTLF
121: VIWRIEHKNP DAMWLWVTSI AGEFWFGFSW LLDQLPKLNP INRVPDLAVL RRRFDHADGT
181: SSLPGLDIFV TTADPIKEPI LSTANSILSI LAADYPVDRN TCYLSDDSGM LLTYEAMAEA
241: AKFATLWVPF CRKHAIEPRG PESYFELKSH PYMGRAQEEF VNDRRRVRKE YDDFKARING
301: LEHDIKQRSD SYNAAAGVKD GEPRATWMAD GSQWEGTWIE QSENHRKGDH AGIVLVLLNH
361: PSHARQLGPP ASADNPLDFS GVDVRLPMLV YVAREKRPGC NHQKKAGAMN ALTRASAVLS
421: NSPFILNLDC DHYINNSQAL RAGICFMLGR DSDTVAFVQF PQRFEGVDPT DLYANHNRIF
481: FDGTLRALDG LQGPIYVGTG CLFRRITLYG FEPPRINVGG PCFPRLGGMF AKNRYQKPGF
541: EMTKPGAKPV APPPAATVAK GKHGFLPMPK KAYGKSDAFA DTIPRASHPS PYAAEAAVAA
601: DEAAIAEAVM VTAAAYEKKT GWGSDIGWVY GTVTEDVVTG YRMHIKGWRS RYCSIYPHAF
661: IGTAPINLTE RLFQVLRWST GSLEIFFSRN NPLFGSTFLH PLQRVAYINI TTYPFTALFL
721: IFYTTVPALS FVTGHFIVQR PTTMFYVYLA IVLGTLLILA VLEVKWAGVT VFEWFRNGQF
781: WMTASCSAYL AAVLQVVTKV VFRRDISFKL TSKLPAGDEK KDPYADLYVV RWTWLMITPI
841: IIILVNIIGS AVAFAKVLDG EWTHWLKVAG GVFFNFWVLF HLYPFAKGIL GKHGKTPVVV
901: LVWWAFTFVI TAVLYINIPH IHGPGRHGAA SPSHGHHSAH GTKKYDFTYA WP