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8.A.20.1.1
WR3; wound responsive protein-3 or NAR2.1, an accessory protein for transport via 2.A.1.8.12 (Yong et al., 2010). It has 210 aas and 2 TMSs, N- and C-terminal. It may act as a dual component transporter with NTR2.1 and is required for high-affinity nitrate transport. It acts as a repressor of lateral root initiation and may be involved in targeting NRT2 proteins to the plasma membrane.

Accession Number:Q9FGS5
Protein Name:High-affinity nitrate transporter 3.1
Length:210
Molecular Weight:23403.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Cell membrane1 / Single-pass membrane protein2
Substrate

Cross database links:

Entrez Gene ID: 835085   
KEGG: ath:AT5G50200   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015112 F:nitrate transmembrane transporter activity
GO:0042128 P:nitrate assimilation
GO:0010167 P:response to nitrate
GO:0009611 P:response to wounding

References (7)

[1] “Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.”  Sato S.et.al.   10718197
[2] “Repression of nitrate uptake by replacement of Asp105 by asparagine in AtNRT3.1 in Arabidopsis thaliana L.”  Kawachi T.et.al.   16980702
[3] “High-affinity nitrate transport in roots of Arabidopsis depends on expression of the NAR2-like gene AtNRT3.1.”  Okamoto M.et.al.   16415212
[4] “Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction.”  Orsel M.et.al.   17012411
[5] “Regulation of root nitrate uptake at the NRT2.1 protein level in Arabidopsis thaliana.”  Wirth J.et.al.   17573350
[6] “Nitrate signaling and the two component high affinity uptake system in Arabidopsis.”  Orsel M.et.al.   19704673
[7] “Characterization of an intact two-component high-affinity nitrate transporter from Arabidopsis roots.”  Yong Z.et.al.   20561257

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MAIQKILFAS LLICSLIQSI HGAEKVRLFK ELDKGALDVT TKPSREGPGV VLDAGKDTLN 
61:	ITWTLSSIGS KREAEFKIIK VKLCYAPPSQ VDRPWRKTHD ELFKDKTCPH KIIAKPYDKT 
121:	LQSTTWTLER DIPTGTYFVR AYAVDAIGHE VAYGQSTDDA KKTNLFSVQA ISGRHASLDI 
181:	ASICFSVFSV VALVVFFVNE KRKAKIEQSK