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8.A.30.2.1
Metal homeostasis protein, Bsd2, of 321 aas and 2 or 3 TMSs. It is required for the homeostasis of heavy metal ions such as cadmium, cobalt and copper. It controls metal ion transport and prevents metal hyperaccumulation by negatively regulating the SMF1 and SMF2 metal transport systems (TC#s 2.A.55.1.1 and .1.2, respectively). Under manganese-replete conditions, Bsd2 facilitates trafficking of SMF1 and SMF2 metal transporters to the vacuole where they are degraded (Liu et al. 1997; Liu and Culotta 1999; Stimpson et al. 2006). Bsd2 also binds the ubiquitin ligase Rsp5 to mediate the ubiquitination of transmembrane proteins (Hettema et al. 2004).  

Accession Number:P38356
Protein Name:Bsd2 aka YBR290W aka YBR2037
Length:321
Molecular Weight:35764.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Endoplasmic reticulum1 / Multi-pass membrane protein2
Substrate

Cross database links:

DIP: DIP-7753N
RefSeq: NP_009849.1   
Entrez Gene ID: 852593   
Pfam: PF10176   
KEGG: sce:YBR290W   

Gene Ontology

GO:0005783 C:endoplasmic reticulum
GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0005515 F:protein binding
GO:0030001 P:metal ion transport
GO:0006623 P:protein targeting to vacuole
GO:0006950 P:response to stress
GO:0006511 P:ubiquitin-dependent protein catabolic process

References (10)

[1] “The requirement for yeast superoxide dismutase is bypassed through mutations in BSD2, a novel metal homeostasis gene.”  Liu X.F.et.al.   7935419
[2] “The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae.”  Holmstroem K.et.al.   8091861
[3] “Complete DNA sequence of yeast chromosome II.”  Feldmann H.et.al.   7813418
[4] “Negative control of heavy metal uptake by the Saccharomyces cerevisiae BSD2 gene.”  Liu X.F.et.al.   9115231
[5] “Post-translation control of Nramp metal transport in yeast. Role of metal ions and the BSD2 gene.”  Liu X.F.et.al.   9988727
[6] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[7] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[8] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[9] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[10] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MPEQELLIGQ EMNTLHAGSS TDGINVGNAG RTRDTQTGVE GETEIGSDEE DSIEDEGSSS 
61:	GGNSTTERLV PHQLREQAAR HIGKIGRHFN ILDRLFKKRT QQSSDIQQGA MFDGVFSNLS 
121:	AKPDTTETEG NNEQDIPPTY DEAAADMAPS YYGMDLNNSD IYYDEICIEG LPVGNIANLL 
181:	WNIIVSTSFQ FIGFLITYIL HTSHAAKQGS RFGLGLTFIG YGYSMIPNDV TSKVGKNKSL 
241:	NRMELEDPNE FDDVRLNSQS TTQDKFESHL NHGLDEEKQN IPWLAVFVAF LGLFITLKSI 
301:	YDYIQVKKLE KKYLNQSQNQ A