8.A.43.1.12
Internalin-A, InlA, of 800 aas and 2 TMSs, N- and C-terminal. L. monocytogenes virulence factors include two surface invasins,
InlA and InlB, known to promote bacterial uptake by host cells, and the
secreted pore-forming toxin listeriolysin O (LLO), which disrupts the phagosome to allow bacterial proliferation in the cytosol. No role for InlB was detected in any tested cells unless the InlB
expression level was substantially enhanced, which was achieved by
introducing a mutation (prfA*) in the gene encoding the
transcription factor PrfA (Phelps et al. 2018). InlA and LLO were the most
critical invasion factors. InlA facilitates both
bacterial attachment and internalization in cells that express its
receptor, E-cadherin. LLO promotes L. monocytogenes internalization into hepatocytes, but not into cytotrophoblasts and
endothelial cells. LLO and InlA cooperate to increase the
efficiency of host cell invasion by L. monocytogenes (Phelps et al. 2018).
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| Accession Number: | P0DJM0 |
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| Protein Name: | Internalin-A |
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| Length: | 800 |
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| Molecular Weight: | 86493.00 |
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| Species: | Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [169963] |
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| Number of TMSs: | 2 |
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| Location1 / Topology2 / Orientation3: |
Secreted1 / Peptidoglycan-anchor2 |
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| Substrate |
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1: MRKKRYVWLK SILVAILVFG SGVWINTSNG TNAQAATITQ DTPINQIFTD TALAEKMKTV
61: LGKTNVTDTV SQTDLDQVTT LQADRLGIKS IDGVEYLNNL TQINFSNNQL TDITPLKNLT
121: KLVDILMNNN QIADITPLAN LTNLTGLTLF NNQITDIDPL KNLTNLNRLE LSSNTISDIS
181: ALSGLTSLQQ LSFGNQVTDL KPLANLTTLE RLDISSNKVS DISVLAKLTN LESLIATNNQ
241: ISDITPLGIL TNLDELSLNG NQLKDIGTLA SLTNLTDLDL ANNQISNLAP LSGLTKLTEL
301: KLGANQISNI SPLAGLTALT NLELNENQLE DISPISNLKN LTYLTLYFNN ISDISPVSSL
361: TKLQRLFFYN NKVSDVSSLA NLTNINWLSA GHNQISDLTP LANLTRITQL GLNDQAWTNA
421: PVNYKANVSI PNTVKNVTGA LIAPATISDG GSYTEPDITW NLPSYTNEVS YTFSQPVTIG
481: KGTTTFSGTV TQPLKAIFNV KFHVDGKETT KEVEAGNLLT EPAKPVKEGH TFVGWFDAQT
541: GGTKWNFSTD KMPTNDINLY AQFSINSYTA TFDNDGVTTS QTVDYQGLLQ EPTAPTKEGY
601: TFKGWYDAKT GGDKWDFATS KMPAKNITLY AQYSANSYTA TFDVDGKSTT QAVDYQGLLK
661: EPKAPTKAGY TFKGWYDEKT DGKKWDFATD KMPANDITLY AQFTKNPVAP PTTGGNTPPT
721: TNNGGNTTPP SANIPGSDTS NTSTGNSAST TSTMNAYDPY NSKEASLPTT GDSDNALYLL
781: LGLLAVGTAM ALTKKARASK