9.A.57.1.12
Smith et al. 2024 showed that ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion. Tricalbin 3 of 1545 aas and possibly 1 or 2 TMSs, near the N-terminus of the protein may be involved. ER-plasma membrane contact sites deliver ER lipids and proteins for rapid cell surface expansion (Smith et al. 2024). As a consequence of hypoosmotic shock, yeast cells swell rapidly and
increase their surface area by ∼20% in 20 s. Approximately, 35% of this
surface increase is mediated by the ER-plasma membrane contact sites,
specifically the tricalbins, which are required for the delivery of both
lipids and the GPI-anchored protein Crh2 from the cortical ER to the
plasma membrane. Therefore, Smith et al. 2024 proposed a new function for the
tricalbins: mediating the fusion of the ER to the plasma membrane at
contact sites. This proposed fusion is triggered by calcium influx via
the stretch-gated channel Cch1 (TC# 1.A.1.11.10) and is supported by the anoctamin Ist2 (TC# 1.A.17.1.19).
|
| Accession Number: | Q03640 |
|---|
| Protein Name: | Tricalbin-3 |
|---|
| Length: | 1545 |
|---|
| Molecular Weight: | 171076.00 |
|---|
| Species: | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292] |
|---|
| Number of TMSs: | 1 |
|---|
| Location1 / Topology2 / Orientation3: |
Cell membrane1 / Multi-pass membrane protein2 |
|---|
| Substrate |
lipid |
|---|
1: MTGIKAQVHP PPDSTLFHEE EKKKVGGNLP QKVINQQERG SDHAPSGHHQ YHQLINHDAN
61: DTKTSNSVSD VSKGQKTADS NPEGKKQSSK DIFVASSAQK TNQLPGPNPQ GSIGAVPLEG
121: LRPKEFRSAP SRKPNKFDTS ITKPGVLDDL GKLDEKDIKE KFHLDSDDKL FPWQNVGEFH
181: ASGKGSPNTK MSRVIKAYIL ENFYNDWYCN IATVLGTCFF SWLFAYIGFS WWSMIFIFLG
241: TATVYNAEYT RFNRNIRDDL KRVTVEETLS DRVESTTWLN SFLSKFWVIY MPVLSQQVKD
301: NVNPQLAGVA PGYGIDALAI DEFTLGSKAP TIKGIKSYTK TGKNTVEMDW SFAFTPSDVS
361: DMTATEAREK INPKISLGVT LGKSFVSKTM PILVEDINVA GKMRIKVEFG KAFPNIKIVS
421: LQLLEPPLID FALKPIGGDT LGLDVMSFLP GLKSFVKNII NSNIGPMLFP PNHLDINVED
481: IMAAQSKEAI GVLAVTIASA DSLKGSDFIT NTVDPYIVMT TEDAVPGTDE EVRTSIKSNV
541: KNPRWNETKY LLLNTLEQKL NLKCFDFNDV RKDTVIGDLQ LDLADLLQNP VLDNQTAELR
601: SGTKSKGILH YSLHWFPVKE DKSEEKAVER AEAKAKGKKE DENEDTTEKE EDENEESSQT
661: DVGIAKITLQ KVKYLDTTSS MTGSLSPCAE LFIDGQKVKS YRTLRRINEP SWNETIEVLV
721: PSKSNSKFVL KIFDDRMNGK ALICEYSSSL DDIMTTLDTA QEFVKGSPQG DIYLDVSWKS
781: IEMTGAFAAA NSVSEPIGCI KLDVKDAIIK GDLSGVGDVD PYYTVSLNRR VLYKSIYHSD
841: TDHPIFDNST YVPIFSPNQI LTLEFHDYQK IGKDRFIGSV QIPTSNVFKK DPKSGKYVGN
901: NGKEEISKLK LKDHEHKVTE SIVNVSTTFI PINLVYSPEE LVNVEKLEKE LKEKKKKFEA
961: TQEENEQEME KNPKEWEVAE IEDPFDSDEK KINRKAKLSL NELIKQKSGI LSMQILEGTL
1021: SPSSAYLEIL ADDISYPVFI CMKPSQGKLN SEMANIFIRD LNYSKLHFRV SKKHIAKDSD
1081: DVISETSYST LKLLKQAYEE PMWLNFNGSK MKVRFLYTPT SVKLPSSESV EDTGYLNIKL
1141: ISGHGLKSAD RNGYSDPFVH IFVNDKKVFK SNIKKKTLDP VWNEDAKIPI LSRSKNQVIF
1201: NVLDWDRAGD NDDLGQASLD VSSLEVGKTY NWNLNLNTQG SIKLQGSFNP EYIKPSFDIV
1261: KGGITDKPMK IASGAAHATV GIAGTGIGAA TGVATGGLKK GGHLLKSLGG NPMKRSKSSN
1321: GNESNGAKKS SEKKSFDRRS PSNLNSTSVT PRASLDYDPS VPNTSYAPVQ SASPVVKPTD
1381: NTSSSSNKKD TPSSNSRGHS RASSFARTLA PHGTYNGFIT VVAAENVAKH VQIKISLTQG
1441: GRLKHIYKTK SQKANNDGVA VFDEECSFKA SPEANLVLGA ISHQRLSRDK DLGIAQINLG
1501: DPQIQQDGQI SVKLGDGHLI VKINYGKDKN GQVPPVPEVP QEYTQ