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| Accession Number: | P36051 |
| Protein Name: | Mcd4 |
| Length: | 919 |
| Molecular Weight: | 105693.00 |
| Species: | Saccharomyces cerevisiae (Baker's yeast) [4932] |
| Number of TMSs: | 14 |
| Location1 / Topology2 / Orientation3: | Endoplasmic reticulum membrane1 / Multi-pass membrane protein2 |
| Substrate | ATP |
Cross database links:
| DIP: | DIP-8272N |
|---|---|
| RefSeq: | NP_012756.1 |
| Entrez Gene ID: | 853690 |
| Pfam: | PF01663 PF04987 |
| KEGG: | sce:YKL165C |
Gene Ontology
GO:0005789
C:endoplasmic reticulum membrane
GO:0009277
C:fungal-type cell wall
GO:0000324
C:fungal-type vacuole
GO:0000139
C:Golgi membrane
GO:0016021
C:integral to membrane
GO:0005774
C:vacuolar membrane
GO:0051377
F:mannose-ethanolamine phosphotransferase act...
GO:0005515
F:protein binding
GO:0015867
P:ATP transport
GO:0007047
P:cellular cell wall organization
GO:0006506
P:GPI anchor biosynthetic process
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References (11)[1] “DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae.” Vandenbol M.et.al. 8091859 [2] “Complete DNA sequence of yeast chromosome XI.” Dujon B.et.al. 8196765 [3] “MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast.” Gaynor E.C.et.al. 10069808 [4] “A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast.” Packeiser A.N.et.al. 10514566 [5] “A genetic screen for ethanolamine auxotrophs in Saccharomyces cerevisiae identifies a novel mutation in Mcd4p, a protein implicated in glycosylphosphatidylinositol anchor synthesis.” Storey M.K.et.al. 11470244 [6] “Correct GPI-anchor synthesis is required for the incorporation of endoglucanase/glucanosyltransferase Bgl2p into the Saccharomyces cerevisiae cell wall.” Kalebina T.S.et.al. 12023081 [7] “ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase.” Zhong X.et.al. 12807869 [8] “Global analysis of protein localization in budding yeast.” Huh W.-K.et.al. 14562095 [9] “Deletion of MCD 4 involved in glycosylphosphatidylinositol (GPI) anchor synthesis leads to an increase in beta-1,6-glucan level and a decrease in GPI-anchored protein and mannan levels in the cell wall of Saccharomyces cerevisiae.” Maneesri J.et.al. 16233801 [10] “Ethanolaminephosphate side chain added to glycosylphosphatidylinositol (GPI) anchor by mcd4p is required for ceramide remodeling and forward transport of GPI proteins from endoplasmic reticulum to Golgi.” Zhu Y.et.al. 16704983 [11] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.” Albuquerque C.P.et.al. 18407956
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External Searches:
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Analyze:
| Predict TMSs (Predict number of transmembrane segments) | ||||
| FASTA formatted sequence |
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1: MWNKTRTTLL AVGVLFHLFY LWSIFDIYFI SPLVHGMSPY QSTPTPPAKR LFLIVGDGLR 61: ADTTFDKVTH PVSGKTEFLA PFIRSLVMNN ATYGISHTRM PTESRPGHVA MIAGFYEDVS 121: AVTKGWKSNP VNFDSFFNQS THTYSFGSPD ILPMFKDGAS DPNKVDTWMY DHTFEDFTQS 181: SIELDAFVFR HLDQLFHNST LNSTLDYEIR QDGNVFFLHL LGCDTAGHSY RPYSAEYYDN 241: VKYIDDQIPI LIDKVNKFFA DDKTAFIFTA DHGMSAFGSH GDGHPNNTRT PLVAWGAGLN 301: KPVHNPFPVS DNYTENWELS SIKRNDVKQA DIASLMSYLI GVNYPKNSVG ELPIAYIDGK 361: ESDKLAALYN NARSILEQYL VKQDEVIDSQ FFYKEYFKFV EKSHSHYLEE IETLIQRISE 421: GENYLEQEAI TLTEELMQIT LEGLHYLTTY NWRFIRTIVT FGFVGWIFFS FIIFLKSFIL 481: ENVIDDQKAS PLSHAVFGSI GILLNWILFY QHSPFNFYMY LLFPLYFWSY IFTNRSVLRS 541: GIKEFFKGTS PWKRVLITIS IISVYEGIVY GFFHRWTFTL ITNILAFYPF ICGVRELSVN 601: ILWIITSVLL STFTLFDAVK IEDLNQIHLA GLLIILSAFY ALYKIHSRIN SYTRAIFAIQ 661: ISLVAAMLAV THRSVISLQL RQGLPRESQV AGWIIFFVSL FVMPILHYRK PNNDYKVRLL 721: IIYLTFAPSF IILTISFESL FYFLFTSYMV QWIEIENKIK EMKTQKDENW LQVLRVSVIG 781: FFLLQVAFFG TGNVASISSF SLESVCRLLP IFDPFLMGAL LMLKLIIPYG LLSTCLGILN 841: LKLNFKDYTI SSLIISMSDI LSLNFFYLLR TEGSWLDIGI TISNYCLAIL SSLFMLILEV 901: LGHVLLKNVI IQDKTKKTQ