*1.B.89. The Gordonia* Outer Membrane Porin (GjpA) Family** Gordonia jacobaea, belonging to the mycolata group, includes mycolic acids in its cell wall that reduce the permeability of its envelope, requiring the presence of channel-forming proteins to allow the exchange of hydrophilic molecules with the surrounding medium. A cell-wall protein with channel-forming activity, has an average single-channel conductance of 800 pS in 1 M KCl, is moderately anion-selective, and does not show a voltage dependence for voltages between +100 and -100 mV. The channel characteristics suggest that this protein could be of relevance in the import and export of negatively charged molecules across the cell wall (Jiménez-Galisteo et al. 2017).
This family belongs to the Actinobacterial Outer Membrane Porin (A-OMP) Superfamily.
References:
Jiménez-Galisteo, G., E. Fusté, E. Muñoz, T. Vinuesa, T.G. Villa, R. Benz, A. Domínguez, and M. Viñas. (2017). Identification and characterization of a cell wall porin from Gordonia jacobaea. J Gen Appl Microbiol 63: 266-273.
Examples:
TC#	Name	Organismal Type	Example
1.B.89.1.1	The pore-forming protein, GjpA of 558 aas and up to 9 TMSs in a 1 (N-terminal) + 2 + 2 + 2 + 2 TMS arrangement with a fairly long (~ 120 aas) hydrophilic C-terminal domain. The channel has an average single-channel conductance of 800 pS in 1 M KCl, is moderately anion-selective, and does not show a voltage dependence for voltages between +100 and -100 mV. GjpA may import and export negatively charged molecules across the outer cell envelope (Jiménez-Galisteo et al. 2017).		GjpA of Gordonia jacobaea

1.B.89.1.10	Uncharacterized protein of 524 aas and ~ 8 putative TMSs. TC BLAST with this protein brings up TC# 9.B.94.1.5 with a score of 0.0066 ( residues 198 - 318 aligning with residues 224 -351 in the protein with TC# 9.B.94.1.5.		UP of Mycobacterium cookii

1.B.89.1.11	PE family protein of 556 aas and ~ 8 putative TMSs. In TC BLAST, this protein hits9.B.94.1.2 with an e-value of e^-6 with filters, aligning residues 45 - 122 with residues 45 - 118 in 9.B.94.1.2.		PE family protein of Mycobacterium ulcerans

1.B.89.1.12	PE family protein of 469 aas and ~ 8 putative TMSs. This protein brings up homologues of 1.B.89, 9.B.96 and 1.B.94 (the PE proteins of the latter systems).		PE family protein of Mycobacterium gordonae

1.B.89.1.13	PE family protein of 1018 aas and ~ 8 putative TMSs.		PE family protein of Mycobacterium canettii

1.B.89.1.14	PE family protein of 914 aas and up to ~12 TMSs.		PE family protein of Mycobacterium tuberculosis

1.B.89.1.15	Uncharacterized protein of 486 aas and ~8 TMSs		UP of Mycolicibacterium flavescens

1.B.89.1.16	Uncharacterized protein of 521 aas and ~ 8 TMSs.		UP of Mycolicibacterium hodleri

1.B.89.1.17	Uncharacterized protein of 370 aas and ~ 8 TMSs		UP of Mycolicibacterium porcinum

1.B.89.1.18	PE family protein of 325 aas and ~8 TMSs		PE protein of Mycobacterium tuberculosis

1.B.89.1.19	Uncharacterized protein of 450 aas and ~8 TMSs.		UP of Mycobacterium grossiae

1.B.89.1.2	Uncharacterized protein with 476 aas and up to 8 predicted TMSs.		UP of Mycolicibacterium smegmatis

1.B.89.1.20	Uncharacterized protein of 399 aas and ~ 10 TMSs.		UP of Mycobacterium botniense

1.B.89.1.21	Putative porin protein encoded bya gene adjacent to one encoding a 6 TMS protein, YvaB, that may function in the export of a peptide toxin. The exporter has TC# 9.A.31.1.2.		YvaB of Streptomyces coelicolor

1.B.89.1.22	The PE12 protein of 308 aas with possibly 3 - 5 α-helical TMSs. PE12 can suppress apoptosis and inhibit the proinflammatory cytokine response. PE12 is related to macrophage phagocytosis (Xu et al. 2024).		PE12 of Mycobacterium tuberculosis

1.B.89.1.23	PE-PGRS family protein of 571 aas	Actinobacteria	PE-PGRS protein of Mycobacteria liflandii

1.B.89.1.24	PE-PGRS family protein of 403 aa	Actinobacteria	PE family protein of Mycobacterium liflandii

1.B.89.1.3	PE family protein of 503 aas and up to 10 TMSs. The PE family is described under TC# 9.B.96 and is also called the PE-PGRS Family.		PE family protein of Mycobacterium gordonae

1.B.89.1.4	Uncharacterized protein of 482 aas and up to 8 putative TMSs.		UP of Gordonia polyisoprenivorans

1.B.89.1.5	Uncharacterized protein of 472 aas and ~ 6 putative TMSs.		UP of Mycolicibacterium porcinum

1.B.89.1.6	Uncharacterized protein of 462 aas and ~ 8 putative TMSs		UP of Mycobacterium aquaticum

1.B.89.1.7	Uncharacterized protein of 321 aas and ~ 8 TMSs.		UP of Mycobacterium eburneum

1.B.89.1.8	Uncharacterized protein of 506 aas and ~ 8 putative TMSs.		UP of Mycolicibacterium aubagnense

1.B.89.1.9	Uncharacterized protein of 766 aas and more than 8 potential TMSs.		UP of Mycolicibacter senuensis

Examples:
TC#	Name	Organismal Type	Example
1.B.89.2.1	Uncharacterized protein of 279 aas and ~6 apparent TMSs asumming an α-helical structure. However, it might have a beta structure as an alternative.		UP of Mycolicibacter heraklionensis

1.B.89.2.2	Uncharacterized protein of 21- aas and 2 TMSs.		UP of Mycolicibacter arupensis

1.B.89.2.3	Uncharacterized protein of 233 aas and 4 or more TMSs		UP of Mycobacterium shimoidei

1.B.89.2.4	PE-PPE domain-containing protein of 508 aas and ~8 TMSs.		PE-PPE domain protein of Mycolicibacter icosiumassiliensis

Examples:
TC#	Name	Organismal Type	Example