*1.E.61. The Pseudomonas* phage phiKMV (PpKMV holin) Family** This family is small with only a few proteins, and the holins are also small, of about 66 aas with a single TMS.
References:
Briers, Y., L.M. Peeters, G. Volckaert, and R. Lavigne. (2011). The lysis cassette of bacteriophage ϕKMV encodes a signal-arrest-release endolysin and a pinholin. Bacteriophage 1: 25-30.
Rathor, N., C.K. Thakur, B.K. Das, and R. Chaudhry. (2022). An insight into the therapeutic potential of a novel lytic Pseudomonas phage isolated from the river Ganga. J Appl Microbiol. [Epub: Ahead of Print]
Examples:
TC#	Name	Organismal Type	Example
1.E.61.1.1	Holin (gene 44) of Pseudomonas phage phiKMV of 66 aas and 1 TMS. The holin accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of nanometer-scale pores (pinholes) causing host cell membrane depolarization and a protein, endolysin orcytolysin refolding and release into the periplasmic space (Briers et al. 2011). The 66 aa holin from Pseudomonas phage AIIMS-Pa-Ai (genbank acc # QPP21131.1) is identical to this one (Rathor et al. 2022). An identical protein in the region of overlap has 77 aas (Rathor et al. 2022).		Holin of phage phiKMV

1.E.61.1.2	Holin homologue of 79 aas and 1 TMS.		Holin of Pseudomonas tolaasii