2.A.102 The 4-Toluene Sulfonate Uptake Permease (TSUP) Family
The TSUP family is also referred to as the TauE/SafE/YfcA/DUF81/COG0730 Family. Although its members have not been rigorously characterized, evidence is available that at least some members function in the transport of sulfur containing organic compounds. These include sulfolactate which may be exported by the TauE protein of Cupriavidus necator (Weinitschke et al. 2007; Mayer et al. 2012) and sulfoacetate which may be exported by the SafE1 protein of Neptuniibacter caesariensis (Krejcík et al. 2008). Another member of the TSUP family, TsaS of Comamonas testosteroni, has been reported to function in the uptake of 4-toluene sulfonate (Mampel et al. 2004). None of these functional assignments can be considered to be certain.
The TSUP family has been characterized using bioinformatic approaches. Shlykov et al. 2012; showed that prokaryotic members of the family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. Despite unequal representation, homologues from the three taxonomic domains of life share well-conserved motifs. A prototypical eight TMS topology apparently arose by intragenic duplication of a four transmembrane segment (TMS) unit. Possibly, a two TMS α-helical hairpin structure was the precursor of the 4 TMS repeat unit. Genome context analyses confirmed the proposal of a sulfur-based compound transport role for many TSUP homologues, but functional outliers appear to be prevalent as well (Shlykov et al. 2012). The TSUP is an established member of the Transporter/Opsin/G protein-coupled receptor (TOG) superfamily (Yee et al. 2013).
The putative 4-toluenesulfonate uptake permease, TsaS
TsaS of Comamonas testosteroni (Q6A553)
Sulfolactate (sulfite/organosulfonate) exporter,TauE of 256 aas with 8 TMSs in a 4 + 4 TMS arrangement. Catalyzes export of sulfolactate as the terminal step in the metabolism of cysteate (Weinitschke et al. 2007; Mayer et al. 2012).
TauE of Cupriavidus necator (Ralstonia eutropha) (Q0K020)
Uncharacterized protein of 250 aas nad 6 TMSs.
UP of Arthrobacter crystallopoietes
Putative sulfite exporter, the TauE/SafE family protein of 241 aas and 7 or 8 TMSs with a probable 4 + 4 TMS arrangement.
TauE homolog of Adlercreutzia sp.
TauE/SafE family of putative sulfite exporters.
TauE homolog of Hydrogenobaculum sp. (metagenome)
The SafE1 sulfoacetate efflux pump (Krejcík et al., 2008).
SafE of Neptuniibacter caesariensis (Q2BM66)
Transporter of unknown function
Uncharacterized permease of Rhodopirellula baltica
Uncharacterized protein of Rhodopseudomonas palustris
Uncharacterized protein of Streptomyces coelicolor
Putative sulfite exporter, TauE or SafE, of 347 aas and 7 - 8 TMSs.
SafE of Leptospira kmetyi
Uncharacterized protein of 240 aas
UP of Mycobacterium smegmatis
Uncharacterized protein of 245 aas
UP of Achromobacter xylosoxidans
Uncharacterized protein of 291 aas and 8 TMSs. A member of the TSUP or TauE/SafE family.
UP of E. coli
Orf of 269 aas and 6 TMSs
YfcA of E. coli (P0AD30)
Uncharacterized protein of 253 aas and 8 TMSs
UP of Gordonia amicalis
Uncharacterized protein of 251 aas and 6 TMSs (Hug et al. 2016).
UP of Candidatus Peribacter riflensis
Glycine transporter, YfcA, of 269 aas and 9 TMSs (Deutschbauer et al. 2011).
YfcA of Shewanella oneidensis
Orf of 253aas and 8 TMSs
Orf of Pyrococcus abyssi (Q9UYH7)
Uncharacterized protein of 671 aas and 9 TMSs. TMS1 is N-terminal, followed by a peptidase domain of 350 aas, followed by a transmembrane domain with 8 putative TMSs, homologous to other members of this family.
UP of Magnetococcus sp.
Putative permease of 259 aas and 8 TMSs
PP of Bdellovibrio bacteriovorus
Uncharacterized protein of 245 aas and 8 TMSs.
UP of Lactobacillus rhamnosus
Acetate transporter of 266 aas and 8 TMSs, SO_1333 (Deutschbauer et al. 2011).
SO_1333 of Shewanella oneidensis
Uncharacterized membrane protein of 339 aas and 10 TMSs. The gene encoding this protein is adjacent to the ABC exporter with TC# 3.A.1.148.1.
UP of Microbispora sp. ATCC PTA-5024
Uncharacterized protein of 270 aas and 8 TMSs.
UP of candidatus Bathyarchaeota archaeon (marine sediment metagenome)
Possible organo-sulfur-containing compound transporter (Gristwood et al., 2011) (271aas; 8 TMSs; duplicated). May function with two 4 TMS proteins, PmpA and PmpB of the YedE/YeeE family (TC#9.B.102).
PmpC of Serratia sp. (E7BBJ3)
HP of Methanococcus maripaludis
HP of Streptococcus pneumoniae
Putative sulfite exporter of 304 aas and 9 TMSs, TauE/SafE.
TauE homologue of Thalassiobium sp.
Uncharacterized protein of the TauE family of 380 aas and 8 TMSs.
TauE homologue of Bradyrhizobium japonicum
DUF81 protein of 330 aas and 8 TMSs
DUF81 protein of Halobacterium salinarum
The putative sulfate transporter, CysZ (Rückert et al., 2005).
CysZ of Corynebacterium glutamicum (Q8NLX4)
TauE (DUF81) homologue of 322 aas and 9 putative TMSs
TauE homologue of Galdieria sulphuraria
Orf of 590 aas and 12 TMSs
Orf of Oryza sativa (Q5ZAL4)
Uncharacterized protein of 480 aas and 8 TMSs.
UP of Marchantia polymorpha
Uncharacterized protein of 10 - 12 TMSs in a 3 - 5 + 2 + 5 TMS arrangement.
UP of Giardia intestinalis
Putative sulfite exporter,TauE/SafE family protein, of 476 aas and 10 TMSs.
TauE homolog of Arabidopsis thaliana
Putative permease of 208 aas and 6 TMSs
Putative permease of Methylococcaceae bacterium HT3
Uncharacterized protein of 174 aas and 4 TMSs
UP of Strongylus vulgaris
Putative sulfite exporter TauE/SafE family protein of 333 aas and 11 TM
TauE homologue of Desulfobacterium autotrophicum
Putative sulfite exporter TauE/SafE family protein of 331 aas and 9 TMSs.
TauE homolog of Candidatus Filomicrobium marinum
Uncharacterized protein of 318 aas and 9 TMSs.
UP of Pseudomaricurvus alcaniphilus
Putative sulfite exporter TauE/SafE family protein of 307 aas and 9 TM
TauE homologue of Litorilinea aerophila