2.A.2 The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family

GPH:cation symporters catalyze uptake of sugars (mostly, but not exclusively, glycosides) in symport with a monovalent cation (H+ or Na+). Mutants of two groups of these symporters (the melibiose permeases of enteric bacteria and the lactose permease of Streptococcus thermophilus) have been isolated and in which altered cation specificity is observed or in which sugar transport is uncoupled from cation symport (i.e., uniport is catalyzed). The various members of the family can use Na+, H+ or Li, Na+ or Li+, H+ or Li+, or only H+ as the symported cation. Most functionally characterized and sequenced members of the family are from bacteria except the distantly related sucrose:H+ symporters of plants and a yeast maltose/sucrose:H+ symporter of S. pombe. This yeast protein is about 24% identical to the plant sucrose:H+ symporters and is more distantly related to the bacterial members of the GPH family (Reinders and Ward, 2001). Homologues are found in archaea and all eukaryotic kingdoms.

Proteins of the GHP family are generally about 500 amino acids in length, although the Gram-positive bacterial lactose permeases are larger, due to a C-terminal hydrophilic domain that is involved in regulation by the phosphotransferase system (TC #4.A.1). All of these proteins possess twelve The GPH family is a member of the MFS. One member of the GPH family, LacS of Streptococcus thermophilus, appears to be a cooperative dimer with one sugar translocation pathway per monomer (Veenhoff et al., 2001).

X-ray crystal structures of MelBSt have revealed the molecular recognition mechanism for sugar binding. Markham et al. 2021 generated a complete single-Cys library containing 476 unique mutants by placing a Cys at each position on a functional Cys-less background. 105 mutants (21%) exhibited poor transport activities, although the expression levels of most mutants were comparable to that of the control. The affected positions are distributed throughout the protein. TMSs I and X and transmembrane residues, Asp and Tyr, are most affected by cysteine replacement, while helix IX, the cytoplasmic middle-loop, and C-terminal tail are least affected. Single-Cys replacements at the major sugar-binding positions (K18, D19, D124, W128, R149, and W342) or at positions important for cation binding (D55, N58, D59, and T121) abolished the Na+-coupled active transport (Markham et al. 2021).

The generalized transport reaction catalyzed by the GPH:cation symporter family is:

Sugar (out) + [H+ or Na+] (out) → Sugar (in) + [H+ or Na+] (in).

 

 



This family belongs to the Major Facilitator (MFS) Superfamily.

 

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Examples:

TC#NameOrganismal TypeExample
2.A.2.1.1

Melibiose permease. Catalyzes the coupled stoichiometric symport of a galactoside with a cation (either Na+, Li+, or H+). Based on LacY, a 3-d model has been derived (Yousef and Guan, 2009). Asp55 and Asp59 are essential for Na+ binding. Asp124 may play a critical role by allowing Na+-induced conformational changes and sugar binding. Asp19 may facilitate melibiose binding (Granell et al., 2010).  The alternate access mechanism fits better into a flexible gating mechanism rather than the archetypical helical rigid- body rocker-switch mechanism (Wang et al. 2016).  Crystal structures of Salmonella typhimurium MelB in two conformations, representing an outward partially occluded and an outward inactive state (Ethayathulla et al. 2014). MelB adopts a typical MFS fold and contains a previously unidentified cation-binding motif. Three conserved acidic residues form a pyramidal-shaped cation-binding site for Na+, Li+ or H+, which is in close proximity to the sugar-binding site. Both cosubstrate-binding sites are mainly contributed by the residues from the amino-terminal domain (Ethayathulla et al. 2014). The Glucose Enzyme IIA protein of the PTS binds MelB either in the absence or presence of a galactoside, and binding decreases the affinity for melibiose, giving rise to inducer exclusion (Saier 1989; Hariharan and Guan 2014).

Gram-negative bacteria

MelB of E. coli (A7ZUZ0)

 
2.A.2.1.2

Probable fucosyl-α-1,6-N-acetylglucosamine uptake porter, AlfD (next to and in an operon with a fucosidase (AlfA) specific for this disaccharide which is present in mammalian glycoproteins, glycolipids and milk (Rodríguez-Díaz et al. 2012).

Firmicutes

AlfD of Lactobacillus casei

 
2.A.2.1.3

Uncharacterized protein, probably a sugar:H+ symporter of 474 aas and 12 TMSs, YjmB,  The gene was from a marine sediment metagenome.

YjmB of Lokiarchaeum sp. GC14_75

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.2.1

Lactose permease, LacS. Mediates uptake of β-galactooligosaccharides, lactitol, and a broad range of prebiotic β-galactosides that selectively stimulate beneficial gut microbiota (Andersen et al., 2011). 

Gram-positive bacteria

LacS of Streptococcus thermophilus

 
2.A.2.2.2Raffinose permease Gram-positive bacteria RafP of Pediococcus pentosaceus
 
2.A.2.2.3

Galactose permease of 462 aas and 12 TMSs.  Transports galactose (Grossiord et al. 2003).

Gram-positive bacteria

GalP of Lactococcus lactis

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.3.1

Glucuronide permease, UidB, GusB, UidP (Liang et al., 2005; Moraes and Reithmeier 2012)

Gram-negative bacteria

GusB of E. coli

 
2.A.2.3.10Transmembrane protein 180AnimalsTMEM180 of Homo sapiens
 
2.A.2.3.11

Putative transporter

Euglenozoa

Putative transporter of Trypanosoma cruzi

 
2.A.2.3.12

Putative sugar transporter

Bacteria

TT_P0219 pf Thermus thermophilus

 
2.A.2.3.13

Probable sugar transporting MFS-2 symporter of 444 aas and 12 TMSs.

MFS carrier of Candidatus Thorarchaeota archaeon

 
2.A.2.3.14

Probable sugar:cation symporter, MFSD13A or TMEM180, with 517 aas and 12 TMSs with the N- and C-termini reported to be exposed extracellularly (Anzai and Matsumura 2019). It has anti-tumor activity (Yasunaga et al. 2019) and is highly expressed in colorectal cancer (CRC) (Anzai et al. 2021; Shiraishi et al. 2021). It is also a schizophrenia risk factor (Wang et al. 2021).

TMEM180 of Homo sapiens

 
2.A.2.3.15

Probable sulfoquinovose importer of 467 aas and 12 TMSs (Denger et al. 2014). Sulphoquinovose (SQ, 6-deoxy-6-sulphoglucose) is the polar headgroup of the plant sulpholipid in the photosynthetic membranes of all higher plants, mosses, ferns, algae, most photosynthetic bacteria, and some non-photosynthetic bacteria. It is part of the surface layer of some Archaea. The estimated annual production of SQ is 10,000,000,000 tonnes (10 petagrams) (Denger et al. 2014).

Sulfoquinovose importer of E. coli

 
2.A.2.3.16

MfsD2B or SLC59A2 protein of 504 aas and 12 TMSs (). It is a cation-dependent lipid transporter that specifically mediates export of sphingosine-1-phosphate from red blood cells and platelets (Vu et al. 2017). Sphingosine-1-phosphate is a signaling sphingolipid, and its export from red blood cells into in the plasma is required for red blood cell morphology. It does not transport lysophosphatidylcholine (LPC).

MfsD2B of Homo sapiens

 
2.A.2.3.17

Putative sugar: cation symporter, GPH, of 548 aas and 12 TMSs in a 6 + 6 TMS arrangement (Wunderlich 2022).

GPH of Plasmodium falciparum

 
2.A.2.3.2Pentoside permease Gram-positive bacteria XynC (YnaJ) of Bacillus subtilis
 
2.A.2.3.3

Isoprimeverose (α-D xylopyranosyl-(1,6)-D-glucopyranose) permease [xylose is not a substrate] (Heuberger et al., 2001)

Gram-positive bacteria

XylP of Lactobacillus pentosus

 
2.A.2.3.4Probable α-xyloside uptake permease, YicJ (Laikova et al., 2001)BacteriaYicJ of E. coli (P31435)
 
2.A.2.3.5Probable β-xyloside uptake permease, YagG (Laikova et al., 2001)BacteriaYagG of E. coli (P75683)
 
2.A.2.3.6

The putative cellobiose porter, BglT (Rodionov et al. 2010)

Proteobacteria

BglT of Shewanella amazonensis (A1S5F2)

 
2.A.2.3.7

The putative arabinoside porter, AraT (Rodionov et al., 2010)

Proteobacteria

AraT of Shewanella sp. MR-4 (Q0HIQ0)

 
2.A.2.3.8

Major Facilitator Superfamily Domain containing 2A, MFSD2A or SLC59A1 (543aas, 12 TMSs). Plays a role in thermogenesis via β-adrenergic signaling. Takes up Tunicamycin (TM), a mixture of related species of nucleotide sugar analogs fatty-acylated with alkyl chains of varying lengths and degrees of unsaturation, produced by several Streptomyces species (Bassik and Kampmann, 2011; Reiling et al., 2011).  It is a sodium-dependent lysophosphatidylcholine (LPC) symporter expressed at the blood-brain barrier endothelium. It is the primary route for import of docosahexaenoic acid and other long-chain fatty acids into foetal and adult brain, and is essential for mouse and human brain growth and function (Quek et al. 2016). In addition to a conserved sodium-binding site, three structural features were identified: A phosphate headgroup binding site, a hydrophobic cleft to accommodate a hydrophobic hydrocarbon tail, and three sets of ionic locks that stabilize the outward-open conformation. Ligand docking studies and biochemical assays identified Lys436 as a key residue for transport. It forms a salt bridge with the negative charge on the phosphate headgroup. Mfsd2a transports structurally related acylcarnitines but not a lysolipid without a negative charge, demonstrating the necessity of a negative charged headgroup interaction with Lys436 for transport. These findings support a novel transport mechanism by which LPCs are flipped within the transporter cavity by pivoting about Lys436 leading to net transport from the outer to the inner leaflet of the plasma membrane (Quek et al. 2016). Docosahexaenoic acid is an omega-3 fatty acid that is essential for neurological development and function, and it is supplied to the brain and eyes predominantly from dietary sources. This nutrient is transported across the blood-brain and blood-retina barriers as lysophosphatidylcholine. The structure of MFSD2A has been determined using single-particle cryo-EM (Cater et al. 2021). The transporter is in an inward-facing conformation and features a large amphipathic cavity that contains the Na+-binding site and a bound lysolipid substrate. This structure reveals details of how MFSD2A interacts with substrates and how Na+-dependent conformational changes allow for the release of these substrates into the membrane through a lateral gate. This atypical MFS transporter mediates the uptake of lysolipids into the brain. Homozygous variants in the MFSD2A gene cause severe primary microcephaly, brain malformations, developmental delay, and epilepsy (Khuller et al. 2021). Bi-allelic MFSD2A variants cause autosomal recessive primary microcephaly type 15 and broaden the phenotypic spectrum associated with these pathogenic variants, emphasizing the role of MFSD2A in early brain development.

Animals

MFSD2A of Homo sapiens (Q8NA29)

 
2.A.2.3.9

Inner membrane symporter YihP

Bacteria

YihP of E. coli

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.4.1

Sucrose:H+ symporter, Suc1 or Sut1. It provides osmotic driving force for anther dehiscence, pollen germination and pollen tube growth and also transports other glucosides such as maltose and phenylglucosides. Km (sucrose)= 0.5 mM. (Stadler et al., 1999)).  In wheat (Triticum aesticum), there are at least three isoforms designated Sut2A, Sut2B and Sut2D (Deol et al. 2013). The ortholog in the common bean, Phaseolus vulgaris (SUT1.1), has been characterized as a high affinity sucrose:H+ symporter (Santiago et al. 2020). SUTs in rice play a role in the apoplastic loading as a major phloem loading strategy (Wang et al. 2021).

 

Plants

Suc1 of Arabidopsis thaliana

 
2.A.2.4.10

Proton:glucose symporter A; proton-associated sugar transporter A  (PAST-A) (present in brain and deleted in neuroblastoma 5 (DNb-5).  Solute carrier family 45 member 1, SLC45A1 (Bartölke et al. 2014).

Animals

SLC45A1 of Homo sapiens

 
2.A.2.4.11

Sucrose transport protein SUT5 (Sucrose permease 5) (Sucrose transporter 5) (OsSUT5) (Sucrose-proton symporter 5). Sucrose transporter proteins (SUTs) play roles in the phloem loading and unloading of sucrose. The SUT gene family was identified in four Solanaceae species (Capsicum annuum, Solanum lycopersicum, S. melongena, and S. tuberosum) and 14 other plant species ranging from lower and higher plants. The analysis was performed by integration of chromosomal distribution, gene structure, conserved motifs, evolutionary relationship and expression profiles during pepper growth under stresses (Chen et al. 2022).

Plants

SUT5 of Oryza sativa subsp. japonica

 
2.A.2.4.12

Sucrose:H+ symporter, SUC5.  Also transports biotin and possibly maltose (Pommerrenig et al. 2012).

Plants

SUC5 of Arabidopsis thaliana

 
2.A.2.4.13

Scratch, orthologue 1, SCRT; SLC45A2; transports sucrose into pigment-containing vesicles or granules.  Mutations give rise to oculocutaneous albinism (Meyer et al. 2011).

Animals

SCRT of Drosophila melanogaster

 
2.A.2.4.14

Melanocyte-specific antigen or melanoma antigen, MatP, Slc45a2, Aim-1, AIM1, at the mouse underwhite locus.  Regulated by a melanocyte-specific transcription factor essential for pigmentation, MITF (Du and Fisher 2002). Mutations in MatP in humans cause oculocutaneous albinism type IV (OCA4), an autosomal recessive inherited disorder which is characterized by reduced biosynthesis of melanin pigmentation in skin, hair and eyes. The MATP protein consists of 530 amino acids which contains 12 TMSs (Kamaraj and Purohit 2016).  The D93N mutation causes oculocutaneous albinism 4 (OCA4), and the L374F mutatioin correlates with light pigmentation in European populations. Corresponding mutations were produced in the related and well-characterized sucrose transporter from rice, OsSUT1, and transport activity was measured by heterologous expression in Xenopus laevis oocytes and 14C-sucrose uptake in yeast. The D93N mutant had completly lost transport activity while the L374F mutant showed a 90% decrease in transport activity, although the substrate affinity was unaffected (Kamaraj and Purohit 2016).  Mutations in MATP protein showed loss of stability and became more flexible, which alter its structural conformation and function (Kamaraj and Purohit 2016).

Aim1 of Mus musculus

 
2.A.2.4.15

Putative glycoside transporter of 401 aas and 12 TMSs.

UP of Entamoeba histolytica

 
2.A.2.4.2

Phloem-localized sucrose:H+ symporter, Sut1 (mediates sucrose uptake or efflux dependent on the sucrose gradient and the pmf; Carpaneto et al., 2005). Sut1 is a sucrose protein symporter. Protons can move in the absence of sucrose (Carpaneto et al., 2010), but upon addition of sucrose, it becomes a symporter.  Arg-188 in the rice orthologue and homologues are essential (Sun and Ward 2012).

Plants

Sut1 of Zea mays (BAA83501)

 
2.A.2.4.3

Sucrose:H+ symporter, Suc3 or Sut3 of 464 aas. Expressed in cells adjacent to the vascular tissue and in a carpel cell layer). Km (sucrose)= 1.9 mM; maltose is a competitor (Meyer et al., 2000).

Plants

Suc3 of Arabidopsis thaliana
(O80605)

 
2.A.2.4.4The brain proton:associated sugar (glucose) transporter, PAST-A (Shimokawa et al., 2002)Animals PAST-A of Rattus norvegicus (Q8K4S3)
 
2.A.2.4.5

The proton:sucrose uptake symporter, Sut1 (Zhang & Turgeon et al., 2009).

Plants

Sut1 of Verbascum phoeniceum (D1GC38)

 
2.A.2.4.6

Vacuolar sucrose;H+ symporter, Suc4, catalyzes sucrose export from vacuoles (Schulz et al., 2011). The interactome of the sucrose transporter, StSUT4, in potato is connected to ethylene and calcium signaling (Garg et al. 2022).

Plants

Suc4 of Arabidopsis thaliana (Q9FE59)

 
2.A.2.4.7

Solute carrier family 45, member 4, SLC45A4.  Transports sucrose by a proton symport mechanism.  Found ubiquitously throughout the tissues of the body (Bartölke et al. 2014).

Animals

SLC45A4 of Homo sapiens

 
2.A.2.4.8

solute carrier family 45, member 3, Slc45A3.  Sucrose:proton symporter associated with prostate cancer and myelination (Bartölke et al. 2014). Four members of the SLC45 family, SLC45A1-SLC45A4, were differentially expressed in melanoma, but only SLC45A2 and SLC45A3 had prognostic guiding values (Xie et al. 2021).

Animals

SLC45A3 of Homo sapiens

 
2.A.2.4.9

Solute carrier family 45, member 2, Slc45A2, also called melanocyte-restricted antigen or melanoma antigen, PatP or Aim1.  Transports sucrose, glucose and fructose with protons, possibly into vesicular structures that contain melanin (Vitavska et al. 2018).  Found in skin and hair; involved in pigmentation (Bartölke et al. 2014).  Defects give rise to oculocutaneous albinism (Meyer et al. 2011). One such mutation in dogs, G493D in TMS 11, gives rise to albinisms (Wijesena and Schmutz 2015). OCA type IV (OCA4, OMIM) develops due to homozygous or compound heterozygous mutations in the solute carrier family 45, member 2 (SLC45A2) gene, and many mutations in this human gene have been identified (Inagaki et al. 2006; Tóth et al. 2017). It interacts with 14-3-3 proteins (see TC# 8.A.98). Multiple pathogenic variants in SLC45A2 give rise to oculocutaneous albinism (Lewis and Girisha 2019). Reviewed by Wiriyasermkul et al. 2020. Four members of the SLC45 family, SLC45A1-SLC45A4, were differentially expressed in melanoma, but only SLC45A2 and SLC45A3 had prognostic guiding values (Xie et al. 2021).

Animals

SLC45A2 of Homo sapiens

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.5.1

Saturated and unsaturated oligogalacturonide transporter, TogT (transports di- to tetrasaccharide pectin degradation products which consist of D-galacuronate, sometimes with 4-deoxy-L-threo-5- hexosulose uronate at the reducing position)

Bacteria TogT of Erwinia chrysanthemi 3937
 
2.A.2.5.2

The putative rhamnogalacturonide porter, RhiT (Rodionov et al. 2004).

Enterobacteria

RhiT of Erwinia carotovora subsp. atroseptica (Q6D188)

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.6.1

Maltose/sucrose H+ : symporter, Sut1 (maltose, Km = 6 %u03BCM; sucrose, Km = 36 %u03BCM)

Yeast

Sut1 of Schizosaccharomyces pombe

 
2.A.2.6.2

The maltose/maltooligosaccharide transporter, MalI (541 aas) (Lohmiller et al., 2008).

Bacteria

MalI of Caulobacter crescentus (Q9A612)

 
2.A.2.6.3

The putative maltose porter, MalT (Rodionov et al., 2010)

Proteobacteria

MalT of Shewanella oneidensis (Q8EEC4)

 
Examples:

TC#NameOrganismal TypeExample
2.A.2.7.1

The insect Bm-re (Bombyx mori red eye) protein; mutants lose ommochromes as well as pigmentation of eggs, eyes, and bodies. May function in pigment transport (Osanai-Futahashi et al., 2012).

Insects

Bm-re of Bombyx mori (I0IYT1)

 
2.A.2.7.2

Bm-re homologue of Tribolium castaneum (Osanai-Futahashi et al., 2012).

Insects

Bm-re homologue of Tribolium castaneum (D6W6W0)

 
2.A.2.7.3

MFSD12, melanosome and lysosome cysteine transporter, of 480 aas and 12 TMSs. It is associated with skin pigmentation in humans, mice, dogs and horses (Crawford et al. 2017; Adhikari et al. 2019; Hédan et al. 2019; Tanaka et al. 2019). Its upregulated expression is observed in melanomas, and elevated MFSD12 levels promote cell proliferation by promoting cell cycle progression (Wei et al. 2019). MFSD12 interference inhibited tumor growth and lung metastasis in melanoma. It mediates the import of cysteine into melanosomes and lysosomes (Adelmann et al. 2020). MFSD12 is required to maintain normal levels of cystine - the oxidized dimer of cysteine - in melanosomes, and to produce cysteinyldopas, the precursors of pheomelanin synthesis made in melanosomes via cysteine oxidation. MFSD12 is necessary for the import of cysteine into melanosomes and, in non-pigmented cells, lysosomes. Loss of MFSD12 reduced the accumulation of cystine in lysosomes of fibroblasts from patients with cystinosis, a lysosomal-storage disease caused by inactivation of the lysosomal cystine exporter, cystinosin (TC# 2.A.43.1.1). Thus, MFSD12 is an essential component of the cysteine importer for melanosomes and lysosomes (Adelmann et al. 2020).

MFSD12 of Homo sapiens