4.D.2 The Glycosyl Transferase 2 (GT2) Family
The GT2 (COG0392) family has been implicated in several distinct functions including drug resistance and various enzymatic activities. Most members have about 8 - 10 TMSs plus a ~340aa C-terminal hydrophilic domain that may have catalytic activity. These proteins are implicated in glycosyl transfer with concomitant export across the membrane. They show very significant similarity to extra N-terminal domains in 3.A.3.4.3 and 4 as well as the C-terminal domain in 2.A.1.3.43 (unpublished results).
UPF0104 of 340 aas and 8 putative TMSs
UPF0104 of Methanococcoides burtonii (Q12TG6)
UPF0104 family protein of 345 aas and 9 or 10 TMSs, in a 5 or 6 + 4 TMS arrangement. Shows extensive similarity to the MFS domains of TC# 3.A.3.4.3 and 4.H.1.2.1.
UPF0104 protein of Chloroflexi bacterium
Nitrogenase molybdenum-iron (NMI) α and β chain-like protein of 347aas and 10 putative TMSs
NMI-like protein of Methanocorpusculum labreanum Z (A2SSY9)
Lysyl phosphatidyl glycerol synthetase
Lysyl-PG synthetase of Archaeoglobus veneficus (F2KRG7)
TerC of 326aas and 10 TMSs
TerC of Selenomonas sp. oral taxon 149 (E0NY51)
Putative ABC transporter (348 aas; 8 TMSs)
Putative ABC transporter of Eubacterium yurii (E0QJ98)
Putative family 2 glycosyl transferase (538 aas; 8 putative TMSs), This protein is designated DPG synthase. Residues 1-220 are in the COGO392 or UPF0108 superfamily while residues 221-538 are in the lysyl phosphatidyl glycerol synthetase domain family). The N-terminal domain of 3.A.3.4.3 (e-8) and an N-terminal domain of 4.D.1.1.3 (e-5) are part of the MFS, but the common domain in TC family 4.D.2 is the lysyl phosphatidyl glycerol synthetase domain.
ORF of Archaeoglobus veneficus (F2KQZ0)
Putative transporter of 348 aas and 8 TMSs. Is encoded together with and probably functions with the ABC transporter TC# 3.A.1.147.6.
Putaqtive transporter of Clostridium difficile
UPF0104 family protein of 387 aas and 7 TMSs.
UPF0104 protein of Ureaplasma parvum
Glycosyl transferase family 2 (GT2; N-terminal) - GtrA 4 TMS domain (C-terminal) protein of 384 aas and 4 - 7 TMSs.
GT2-GtrA protein of Candidatus Zambryskibacteria bacterium
Uncharacterized protein of 344 aas with 1 N-terminal TMS and 3 C-terminal TMSs.
HP of Haloarcula marismotui (Q5UXW9)
Pych_15620 (330aas; 8 TMSs) (Most resembles 4.D.2.2.1 and 4.D.2.1.2).
Pych_15620 of Pyrococcus yayanosii (F8AGU2)
Uncharacterized protein of 331 aas and 8 TMSs.
UP of Bdellovibrio bacteriovorus
Uncharacterized protein of 335 aas and 8 probable TMSs in a 2 + 2 + 4 arrangement.
UP of Methanobacterium subterraneum
Uncharacterized flippase-like domain-containing protein of 243 aas and 8 TM
UP of Thermococcus sp. 4557
Sros_7630 (353aas; 8-9 TMSs) (UPF0164 Superfamily)
Sros_7630 of Streptosporangium roseum (D2ARG4)
Rv0204c (412aas; 8 putative TMSs). May be part of a heme iron acquisition system (Tullius et al. 2011).
Rv0204c of Mycobacterium tuberculosis (O53655)
Varpa_1810 (335aas; 8 TMSs) (TMSs 1-7 resemble TMSs 1-7 of 2.A.1.3.47) (e-10) and 2.A.1.3.44 (e-9) of the MFS).
Varpa_1810 of Variovorax paradoxus (E6V6R4)
YbhN of 318 aas and 8 TMSs
YbhN of E. coli
Uncharaterized protein of 377 aas and 8 TMSs.
UP of Acidithrix ferrooxidans
Cagg_0771 (348aas; 8 TMSs). Shows extensive sequence similarity to 3.A.3.4.3 (N-terminal 300aas; first ~8 TMSs before the ATPase domain) and 2.A.1.3.43 (C-terminal 300aas; last 8 TMSs).
Cagg_0771 of Chloroflexus aggregans (B8G562)
Osct_1517 (341aas; 8 TMSs in a 5+3 arrangement like several of those cited above). (Resembles 4.D.2.1.3 and 6 in addition to 3.A.3.4.3 and 2.A.1.3.43)
Osct_1517 of Oscillochloris trichoides (E1IDW6)
Flippase-like domain-containing protein of 370 aas and 8 TMSs in a 5 + 3 TMS arrangement.
Flippase of Anaerolinea thermophila