8.A.219.  The Proline-rich Anti-Microbial Peptide (PrAMP) Family

Amoung the PrAMPs are a natural peptide, Oncocin of 19 aas (Oncocin, chain 1z; 4Z8C_1z).  In addition to being an antimicrobial peptide that interacts with the ribosome, it also seems to interact with the membrane to enhance the effectiveness of MDR transporters, giving rise to enhanced drug resistance (Peng et al. 2018). A derivative with the same N-terminal sequence is named OM19r and has the sequence:  VDKPPYPRPRPIR(R)PGG(R)-NH2 (Cui et al. 2023). These peptides have been studied in some detail and seem to act synergistically to enhance drug resistance (Schmidt et al. 2016).


 

References:

Cui, Q., H.D. Yu, Q.J. Xu, Y. Liu, Y.T. Wang, P.H. Li, L.C. Kong, H.P. Zhang, X.Y. Jiang, A.M. Giuliodori, A. Fabbretti, C.G. He, and H.X. Ma. (2023). Antibiotic synergist OM19r reverses aminoglycoside resistance in multidrug-resistant. Front Microbiol 14: 1144946.
Peng, S., M. Yang, R.N. Sun, Y. Liu, W. Wang, Q. Xi, H. Gong, and C. Chen. (2018). Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET. Protein Cell 9: 890-895.
Schmidt, R., E. Ostorházi, E. Wende, D. Knappe, and R. Hoffmann. (2016). Pharmacokinetics and in vivo efficacy of optimized oncocin derivatives. J Antimicrob Chemother 71: 1003-1011.
Tereshchenkov, A.G., Z.Z. Khairullina, I.A. Volynkina, D.A. Lukianov, P.A. Nazarov, J.A. Pavlova, V.N. Tashlitsky, E.A. Razumova, D.A. Ipatova, Y.V. Timchenko, D.A. Senko, O.V. Efremenkova, A. Paleskava, A.L. Konevega, I.A. Osterman, I.A. Rodin, P.V. Sergiev, O.A. Dontsova, A.A. Bogdanov, and N.V. Sumbatyan. (2024). Triphenylphosphonium Analogs of Short Peptide Related to Bactenecin 7 and Oncocin 112 as Antimicrobial Agents. Pharmaceutics 16:.
Examples:

TC#NameOrganismal TypeExample
8.A.219.1.1

Oncocin of 19 aas (vdkppylprp rpprriynr) is a proline rich peptide with anti-microbial activity and synergy with MDR transporters. A derivative of oncocin, called OM19r, has the same N-terminal sequence and 19 aas, but a different C-terminal sequence.  See family description for details. Triphenylphosphonium analogs of short peptides related to Bactenecin 7 and Oncocin 112 are antimicrobial agents (Tereshchenkov et al. 2024).

 

Oncocin of Oncopeltus fasciatus (milkweed bug)