9.B.153 The Putative Beta-Barrel Porin/Alpha Amylase (BBP/AA) Family

The BBP/AA family consists of proteins predicted to consist of outer membane beta-barrels. However they are often annotated in the NCBI protein database as the DUF1551 or PF04314 family, alpha-amylase catalytic proteins, members of the Phenol_MetA-deg (MetA-pathway of phenol degradation) superfamily, signal peptide proteins and porins. These proteins are numerous and present in a wide range of Gram-negative bacteria. Some have C-terminal peptidoglycan-binding OmpA-like domains as well as other domains, and have sizes that can be as small as 300 aas and as large as 900 aas. They show sequence similarity with other families such as 9.B.186 and 9.B.170 which may all have an OmpA peptidoglycan-binding domain.  Although these proteins are listed as members of the Outer Membrane Pore-forming Protein (OMPP-I) Superfamily , it is not certain that they show sequence similarity in their putative porin domains.



This family belongs to the Outer Membrane Pore-forming Protein I (OMPP-I) Superfamily .

 

References:

Rouse, S.L., F. Stylianou, H.Y.G. Wu, J.L. Berry, L. Sewell, R.M.L. Morgan, A.C. Sauerwein, and S. Matthews. (2018). The FapF Amyloid Secretion Transporter Possesses an Atypical Asymmetric Coiled Coil. J. Mol. Biol. 430: 3863-3871.

Examples:

TC#NameOrganismal TypeExample
9.B.153.1.1

Phenol_MetA_deg superfamily member with DUF1551 domain signal peptide protein

Planctomycetes

DUF1551 protein of Rhodopirellula baltica

 
9.B.153.1.2

Alpha-amylase domain protein of family PF04314 (355 aas)

Proteobacteria

Uncharacterized protein of Saccharophagus degradans

 
Examples:

TC#NameOrganismal TypeExample
9.B.153.2.1

Putative outer membrane protein of 244 aas

Proteobacteria

Omp of Salmonella enterica

 
9.B.153.2.2

Uncharacterized protein of 304 aas

Proteobacteria

UP of Geobacter daltonii

 
9.B.153.2.3

Uncharacterized protein of 314 aas

Deferribacteres

UP of Denitrovibrio acetiphilus

 
Examples:

TC#NameOrganismal TypeExample
9.B.153.3.1

Protein with C-terminal OmpA domain of 620 aas

Proteobacteria

OmpA-containing protein of Myxococcus xanthus

 
9.B.153.3.10

Putative porin of 265 aas

Armatimonadetes

PP of Chthonomonas calidirosea

 
9.B.153.3.11

Putative porin of 291 aas

Proteobacteria

PP of Desulfobulbus propionicus

 
9.B.153.3.12

Putative porin of 308 aas

Bacteroidetes

PP of Dyadobacter fermentans

 
9.B.153.3.13

Putative porin of 265 aas

Proteobacteria

PP of Sphingomonas wittichii

 
9.B.153.3.14

Putative two domain porin with N-terminal domain homologous to 9.B.153.3.5 and a C-terminal OmpA/MotB domain.

Proteobacteria

PP of Sorangium cellulosum

 
9.B.153.3.15

Putative porin of 523 aas

Planctomycetes

PP of Pirellula staleyi (Pirella staleyi)

 
9.B.153.3.16

Putative porin of 432 aas

Proteobacteria

PP of Stenotrophomonas maltophilia

 
9.B.153.3.17

Putative porin of 423 aas

Bacterial candidate division NC10

PP of Candidatus Methylomirabilis oxyfera

 
9.B.153.3.18

The FapF protein (430 aas) exporter of amyloid subunits, the fibers of which are key components of their biofilm matrix. The secretion of bacterial functional amyloid requires FapF, through which unfolded amyloid substrates are translocated (Rouse et al. 2018).

FapF of Pseudomonas sp. UK4

 
9.B.153.3.2

Thrombospondin type 3 repeat-containing protein of 722 aas

Proteobacteria

Thrombospondin of Nitrosopumilus maritimus

 
9.B.153.3.3

OmpA domain-containing protein of 476 aas

Proteobacteria

OmpA protein of Myxococcus xanthus

 
9.B.153.3.4

Uncharacterized protein of 357 aas

Proteobacteria

UP of Sorangium cellulosum

 
9.B.153.3.5

Uncharacterized protein of 263 aas

Chlorobi

UP of Chlorobaculum parvum (Chlorobium vibrioforme)

 
9.B.153.3.6

Uncharacterized protein of 244 aas

Proteobacteria

UP of Herminiimonas arsenicoxydans

 
9.B.153.3.7

Putative porin of 236 aas

Aquificae

PP of Sulfurihydrogenibium azorense

 
9.B.153.3.8

Putative porin of 290 aas

Proteobacteria

PP of Candidatus Nitrospira defluvii

 
9.B.153.3.9

Putative porin of 259 aas

Verruclmicrobial

Putative porin of Opitutus terrae

 
Examples:

TC#NameOrganismal TypeExample
9.B.153.4.1

Putative porin of 306 aas and 14 putative TMSs.  Belongs to the Phenol MetA deg Pfam family.

Bacteroidetes

PP of Psychroflexus torquis

 
9.B.153.4.2

Putative porin of 315 aas and 12 putative beta strands.

Proteobacteria

PP of Acinetobacter baumannii

 
9.B.153.4.3

Putative porin of 288 aas.

Proteobacteria

PP of Pseudomonas putida

 
9.B.153.4.4

Putative porin of 229 aas

Bacteroidetes

PP of Capnocytophaga canimorsus

 
9.B.153.4.5

Putative porin of 267 aas

Bacteroidetes

PP of Marivirga tractuosa (Microscilla tractuosa) (Flexibacter tractuosus)

 
9.B.153.4.6

Putative porin of 257 aas

Bacteroidetes

PP of Rhodothermus marinus

 
Examples:

TC#NameOrganismal TypeExample
9.B.153.5.1

DUF4270 domain-containing protein of 602 aas and 1 N-terminal TMS.

DUF4270 protein of Robiginitalea biformata