9.B.203 The AIG1 Lipid Hydrolase (AIG1) Family AIG1 and ADTRP are atypical hydrolytic enzymes that depend on conserved threonine and histidine residues for catalysis. Both AIG1 and ADTRP hydrolyze bioactive fatty acid esters of hydroxy fatty acids (FAHFAs) but not other major classes of lipids. Parsons et al. 2016 identified multiple cell-active, covalent inhibitors of AIG1 and showed that these agents block FAHFA hydrolysis in mammalian cells. AIG1 and ADTRP are members of an evolutionarily conserved class of transmembrane threonine hydrolases involved in bioactive lipid metabolism.
References:
Parsons, W.H., M.J. Kolar, S.S. Kamat, A.B. Iii, J.J. Hulce, E. Saez, B.B. Kahn, A. Saghatelian, and B.F. Cravatt. (2016). AIG1 and ADTRP are atypical integral membrane hydrolases that degrade bioactive FAHFAs. Nat Chem Biol 12: 367-372.
Examples:
TC#	Name	Organismal Type	Example
9.B.203.1.1	AIG1, a transmembrane threonine hydrolases of 245 aas and 6 TMSs is involved in bioactive lipid metabolism, specifically, hydrolysis of fatty acid esters of hydroxy fatty acids (Parsons et al. 2016). A possible function in transport has not been studied.		AIG1 of Homo sapiens

9.B.203.1.2	Uncharacterized protein of 257 aas and 6 TMSs		*UP of Echinococcus granulosus* (Hydatid tapeworm)**

9.B.203.1.3	Uncharacterized protein of 246 aas and 6 TMSs		UP of Ajellomyces dermatitidis (Blastomyces dermatitidis)