3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:
The Ycf2-FtsHi complex functions as a
TIC complex-associated ATPase motor in
chloroplast protein import. Cryo-EM
structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20
subunits, including conserved
heterohexameric motor components and
multiple green-algae-specific
components (). This offers evolutionary
insights into the conservation and
diversity of the chloroplast import motor
across species.
- •
Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
- •
ATP binding induces a conformational changes in the ATPase domain of Ctap1.
- •
The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
- •
Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,
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| Accession Number: | 8XQX_A |
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| Protein Name: | A Chain A, Fhl1 |
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| Length: | 1183 |
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| Molecular Weight: | |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 2 |
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| Substrate |
protein |
|---|
1: MQAAMHAQRP AGPPCSSAPS TSYPVAPSPV SSRSRGLHAR RGVAEQRSLG CRSTGSSDQH
61: SNTNDGASGP SRPEQGPELD WSGLPRRQLA AMAMSPFAAL SLPLVNDPAW QQSFETYGGK
121: LREVLLGQQE AAKNVAKQLD EGVTYMDWTY RSTGVDLSAV WDPELWIRFR EAVAQNEPAI
181: FWNKLLDRVQ YKENLPQAGL VGDMRISYAK FLELLKDQRV KRLVVYGDMR TAVVEVPHPW
241: SASVLGHPAT HPFYEDSAHN RVSMLRPNPA APEDVTQWFC AEMPEWDMEK YRFYVDLPGD
301: FWESGVLQRH LAAQRAEGAV WDPASGQYIL PYRAQKKVFQ VSTEVQLLDP QESWDFLGWL
361: LAPGRLEFYE KAACVAIALR VLGIVIAIST GSPLFKLVNV GWGKLRGKGK KNATKDPKKM
421: SKQEKKESQW ERLTSSRARE FMTKDEKTGK MRDTGVRFED IAGMEFLVTE MREIVRMLKG
481: DEAYKRVGAK CPKGIIFQGP PGTGKTYLAR AIAGEAEVPF FSSVGSEFVE MFAGVAAARV
541: NSLFYNARKK APAIIFIDEI DAIGRARSTL GGDPGSMERE SALLAMLVQM DGIANKTEQV
601: LTIGATNLAQ ELDAALLRPG RFEVVYEVPQ PGPSARMAIL RYHAKGKPLE GDGQRLLLKT
661: AEATQGWSAA ALANLMNEAA ILTVRRNVPA ISLPMVLELV EGLNWGEQAP RIPDSEAKDR
721: LALITAAKAV AFALTPGLEP IKSVTMWSGR RGLGPSVDFI AMEDKAAMDM HPEETELMGW
781: RTNFKTNAAV VGDEPLGEFA HVAGLLVPLY AGRAAEVALF GKDGASLATA QPLADCFEIA
841: YYCVRNSQVH PRFKSLPPLH TTMWLGRDDA GRWRRDPLAI GFDEELGYHK LTLTLLKASW
901: RRALRLVAQR RSAITKVAAE MLAAPEEKIT GARLVEIIES TPLDDLGGEG LDGAAAAAVV
961: EEAGNEFLPL LKEVLGQVPG IILTGESLAQ TDDQGRPLPP SSASTSSADA AASDAAASAG
1021: PATELRLDDA TLAAVSRTLM GRLDVVDLIG RNTAVEAAER VRDALLHPET RERLLAMRRW
1081: VEGGPGAPEF PPSPLSPEQT AAMSPSGPLY GNLALNLDWW RRRQDNVISW SAMEILMSRR
1141: QVDLYKQDAD MTEGAIAKLG PPPAAPAAAI GSSSKSSSGQ SS