3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:
The Ycf2-FtsHi complex functions as a
TIC complex-associated ATPase motor in
chloroplast protein import. Cryo-EM
structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20
subunits, including conserved
heterohexameric motor components and
multiple green-algae-specific
components (). This offers evolutionary
insights into the conservation and
diversity of the chloroplast import motor
across species.
- •
Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
- •
ATP binding induces a conformational changes in the ATPase domain of Ctap1.
- •
The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
- •
Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,
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| Accession Number: | 8XQX_B |
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| Protein Name: | B Chain B, Fhl3 |
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| Length: | 1113 |
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| Molecular Weight: | |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 1 |
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| Substrate |
protein |
|---|
1: MRMSGMAIRC AASGSLLASP AASSRPAWRA APVLCSPRVP CTPLELGVSC RRSCMQRRWS
61: RAANVRTLAT SRGEQPQDSG PSTSGRAELP LDSGIGKLIS TTAKAIGLVG LMAVAVLSGP
121: TRAAHARDRL SAQPAAEALI HHQQPYQQPH HHQQQHRSAG AVANPVLSDL AAAPATLEPA
181: TLEPATSTTS ALTPVEAAYS AYLRRIAEAY LAEHPQMAAP EHAAHVARVV RSRALGTPLS
241: FDELMRSAVP APGEVPNRNS RGQVAEQVRA ILDQYDREDF DLGIKQFMLE AKVKAKLEAA
301: SRGTSRDRAA PKDYEEALAA ELFAAEEGAA PKEKAKXEDM VDDAFXXEVV EEAMALFGDA
361: NSVKTAWRTQ EVLRELSYTQ LWALVGEGHV ARVRFYGPEK NKVMATTRAS APGGERLCKV
421: VLPPDPELLD HLVSNGVVVD TGVTEDDRLR ASLLVQMLRY TVPFMVISGL FWMIHTWILD
481: PLPNKFRRQE FIRYRREMLH VASKLNFRTP AREVRIDTGS PDFIKWDDIN GIDEVKKEIN
541: EIIEYLRNPA LLRSRGVARI GGVLLAGAPG TGKTLLAKAI AAEGGVRMFT CSGTDFYDVY
601: SGVGARRVRE TFDRLRNAAP AILFIDEFDA MGAARGAQAS GDESASIINE LLVQMDGFED
661: NRGIVVLGAT NRPGAIDSAL IRPGRFDRII YMPLPDALGR AKIMQVHARN KAVDPNINWY
721: EVARAMAGFT GADVMGLMAR AARMAARQGR HAITEDDIYA AMENKTMEAT LEASTAGDGG
781: GLVGGEGVEG SPDPIPPQLR RAVSVYEAGK ALLAYITPDY EEIARVSVCP LNVLTGFTLF
841: VEDEDKNVNA ILTRSELEGR MVVHLAGRCA EKLVMGEGQM TGMGSPDLFH ANLIAREMIM
901: SMGMGRRTGP IDLLRVAATS EAASGADTLR AGPAAADGDP FYYHTTDMST EQARVALAEV
961: VELLDAAEAK AMYGLAINWR ALQALTQALL DRGTITGKEV AHILESNGVI HFPDPYTTGF
1021: GWDPDGSLRY PFKPDTPPEG GSGGGGAAAE GSAPQTPDLS GARGKTWFAG TAYDAPRNAD
1081: GTFKHGWHWN MPFSVKTELP DWYKKEVERY SY