3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:
The Ycf2-FtsHi complex functions as a
TIC complex-associated ATPase motor in
chloroplast protein import. Cryo-EM
structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20
subunits, including conserved
heterohexameric motor components and
multiple green-algae-specific
components (). This offers evolutionary
insights into the conservation and
diversity of the chloroplast import motor
across species.
- •
Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
- •
ATP binding induces a conformational changes in the ATPase domain of Ctap1.
- •
The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
- •
Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,
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| Accession Number: | 8XQX_D |
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| Protein Name: | D Chain D, Ycf2 |
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| Length: | 2972 |
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| Molecular Weight: | |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Substrate |
protein |
|---|
1: MTFLNHYTYL FSIPEKQADK VSGILRLAQA RPIETLQNER INKQLNAFLK TYKFEKLITN
61: YKKMQSFIPN NSLNGNKTNS STNKLYATSL NVFPENPPLM VRKAVSDEAD KFSKFTYSKV
121: QVVTNNLNNG MNSKEFIKAN NLKPSLRAAE SLVLNHLTYN KFKENLYFKT NNIQPTKSKS
181: TSLFFLNILS NSKPRTCSDF LSSPKIRKTW FRNTAWSLQT QQHRSSNGIN LSLQLPYALG
241: PSVPAGASGQ NMYELPVAQS SSRFGTYYFL QKLLSKYLDV WNASADNGSV LSNSENIKLN
301: FSMVSLLDSK MAIQTPNSLY FVFTQLNQKT FLSYWLLPVA GLALLTPTLL TLTGQSVSVQ
361: KFNSFINKKT DMMVLSNTEM PSKSFGTPTL FGTSVEIYLP NSYMPKGEGE SGINRVNSSI
421: NAVKKNTVTA NLVLDSESQE VATSFQNDLI SIKYCFNNLY NYISNKTALS TKNLFLFSAI
481: KSNATKHKRT QSFFSVENTT TLGNNSNFVK GHFKSSINAF SSYLPSTNVH SMIPLTSLPY
541: LKAISPLYSK FMIDHSLKFI TPKTTLKLLQ HKLNKSPKQM YTKTQNFTGL RDLRALNSFS
601: FGQVNFRTNH FLHSNSRPLN HYNQALKLIN GYEQYKNNLQ INCNKTLDLN TKNKLVYQVN
661: KSHLFNQKCS QIVYKQSLYN RDLCTIRGTG TKVVDYFSHG DKLSNKNGIV LDYFVYSNLL
721: FDNKTNTIIN KDGKQNITKL KLNLTKTTVP FKTLIKKYTS INSLVANEQT RNNLNLGLIH
781: FNGHLSVVSN ANLLTGRPVK FIYYKFDKRL NSYLIYVNQN LKKFIQLNNN FLKPKPLSHQ
841: KNKPVEDFNQ YATNNSSPPK TNVFEKSFVE DSSLRKPLTS LRGSKQFLNS LTILFKHQKM
901: FKKKTLKAHK WHSDTQGIFR KHTNSSFGSA NFSNGPEESS LSTRLHIQKK RKAKKQRLET
961: RRQKKRTRFF PRPVWLRSRM FLNFLTERNK YYLNSTITKQ GFSLPSKDVV TTKLDWLKED
1021: MRRLPLGAYQ YKSLLTQKAG NKFQRQSFTE VVSTMEYING IHKALNNSIF NKIVRKSLLS
1081: SSQNPLKLRL VANYSKMQFM HRVKLPFYRT LKHSEGTKNL ANKKQNLRDI KIKANYNNFK
1141: SQKANNQPQQ NDKDKDKDTM FRDFWVWSYN NTQTNAFNQN LWWLLPNLTT KQSNLEFLTS
1201: TYPTAKETQR AKEEIHGNSI PTASKNQIAL IRLNWALNKT NINTFTDYSK RNNLWTTQKL
1261: RNQSKNNKTK SLEKQFITNW EKFFLNKNLN IFSKKIISKV KQKKQKLNYM TSYLNVQSEH
1321: NVKIFHNSWW THLNIKNLVN NQDMVIPVRE GYFSVGNFNS EFINSAIIKS INNKTLVENY
1381: VYSPSSEKET MQLLLMSSSI LLHLCAIISL VSISQVRCFV KFHLILLYKL SNVYNAILNQ
1441: LSNKLQKNLP IYNNINKLNS RYFYMNHQKS QIKQRKKLLT YFSLTLLKKQ FVTVKPLQIR
1501: NFASIKNQSS NNSNLTYTDM LPLSLRANKF RGSKYDISIR EEEGQSAHIK PSKSMYAKLN
1561: ILSLKTIFLK QLLMNKKPSA LPSNVGLKSN RETQKSQLIQ RIKTKELQIS LKKNIIGFSK
1621: VTKNHILKIL FNVIEVFQTA VRNISSFFEK PAEFTTTWIA YGFLVEWSSD FITIIPENVD
1681: IYIWNVFSKI YRTIPLSFIS TTLGPASTVF DPVTNSTIPI QMGNFNYQKM VAFPILLSLS
1741: HLLHRRILYL FDTLFSTITQ PDTDLIARQE KGTLFWDIWA DFLVTAADYY NVNVAALSTI
1801: KAEQNSLIEN ISNDFDNLTM SSKKPFFMPN KGVSNIKNIF WIKKLKEPQL PESIVQNREV
1861: FVRERKRTLK GLFNIYAPQE ETLWNNPTSP KNLSDEKISF KLFNQLNLQL FAEKNKIKPY
1921: FEAYFSTTQQ KTNIMQSAFP EANLNRWSVN QFITYQSWHS HNGSNNSNGD LFIDYHPPKT
1981: FSHIPALKYN SILQQPIGSL VCQIYSGLFN KQISKNILLV NPKTTSNNLV DYNVLLIQAL
2041: AGETEMKIIT DNAQRYALVN RGFAIGIKLL REVFDAIALN TPCIFLLEDI HAIGERRPML
2101: ISDFGGGMSD DNGSFKEDFF GSQRDEVHEK NQVVYQLTRH AITHYKKPFK GDYSLAIPTN
2161: LYVTDLFLKL PTQSISNLTN VENHNLSIKN KIQHNGTQSL TETKRNLGGD INKNSYLQLT
2221: QFTKTLAPPS TSPFSVLLLK EEKRLKPNKI VEELPWTSLP GEQLATKPRT SYSVRAKVAM
2281: LAELSLSNLS AKLDMITDLL VIIDSVRSNK GFVVFATTDI PHVLDPALRR PGRLDETICL
2341: PNIHTSNILN FTKNYEIFKS AKDTSNFGKK IILNEMQNLT TTSTQRDMYL SCLPTNNQTH
2401: KTKREGVLTM NLKDYNILLN QVYFAEGTGG ILNSQMHKDS LQKSLNFALI SHSKKLKELN
2461: VSKLIGSNGT VSQGNVDQLG VFAGQIVNKQ KKSLQQHLPN SKKSFKKKYK DKAIIYYEVG
2521: KFVLNYFLNN QLTQSSIIDK PVSVTNKQTN DITIFGNDFL NLKTINYLSL YNSKNKILLQ
2581: LMLIFGGKIS QLLSSKNLVK SLKQASINSY MVEEESGSIS SAGMPLGQTH LLPKALSVLA
2641: KPMIFSDGYN NQNLKTATTL LLSFIHKRYL YRKNLIVPKL LSFADGNILD EPPSPPFSSL
2701: LIPAKRFENY KRFFRDTLTG DKMGQRKSQI TLLEKLQYHM QLRSIKQLNA TFSSQENLDF
2761: QSNAALTSQK LDTLMSLSTN NLLQNPTNIN WYYQNRILKR HGQYLTNQWW NGQLSEHNAE
2821: TVFLSDIDWR SSFIKNKNIN ITKSKNLYRL TQQKNNTDGL DVLLDFPDTD QYYNPKRRRW
2881: LLNNGSWNFW FNFDKLYSEE IVTTWILESL IQTYKYLHKN TELLDFVTNK FITLGYIAPE
2941: NANLQNISGF PSQSELLSTK EIILTNSFKR F