3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:
The Ycf2-FtsHi complex functions as a
TIC complex-associated ATPase motor in
chloroplast protein import. Cryo-EM
structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20
subunits, including conserved
heterohexameric motor components and
multiple green-algae-specific
components (). This offers evolutionary
insights into the conservation and
diversity of the chloroplast import motor
across species.
- •
Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
- •
ATP binding induces a conformational changes in the ATPase domain of Ctap1.
- •
The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
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Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,
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| Accession Number: | 8XQX_E |
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| Protein Name: | E Chain E, Ctap1 |
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| Length: | 983 |
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| Molecular Weight: | |
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| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 1 |
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| Substrate |
protein |
|---|
1: MRSAELGRPP RLAQSRLRNV SSHHVTNSCL WLRPPGCRRL VASCAASKES SSASLTAERF
61: ITDAKELNAT GSGLPIIDGP DWEEQHWAAL KAMSAGRPVA LPTPHAKFGP EDLQRIAASG
121: PRLEDLTLEH AERLAGPGQL PTAPDGVALA FRYIPRSVLG DFRQEVEPDW RSLPAMSPAE
181: LYAGLRARNW TSAHYDPAAE PWRLQVFSCD YKHTGVTGWP GYRVVVTSRG GRRRWVDLAE
241: EGELVQLTEQ APPASPADIG YSHVFAQLYQ AYEPRYSPEA LAALYGSSSS KGKAAAAAAA
301: QHDTPALRHL DVSYHGTGSA VAPGSGTAFL MQPSWDAVTG AIRWGLERSG LPELRALRDS
361: LLPEWRPPAL ELNRSNNNLG VVYFAVCLTL GIVIPALRRS RILDIRTLEE DPGAAMEFAR
421: SKSEARKEGL TGVEFRDVAG LGPILNEVVE VVEFLKDPGT FSKLGARPPK GILLEGDPGT
481: GKTLLAKALA GEAMVPFYQM SGTEFTEGIV GLGAARVRDL FKRARATAPC VIFVDEIDAL
541: GLRRAENDSA KTNEEREQTL NQLLTEMDGF TPDTGVVFLG ATNRADLLDP ALMRPGRFDR
601: KIRMPKPDTE GRLEILKLHL RNKQVAPDVD LLQLARDLPG LVGADLANIV NEAAMTAVRS
661: GRQQLTARDI YAGVDRFTQG EVRPSLPTAH KLPVLCFAAK EIGIALVAGE LRDRYGRVEL
721: VERVSIQPKG RAYSRTMFQR GTDEEYQLMT RGRLLDRIRL ALAGGFAVRT ALGEETNFTA
781: ADIKRATRMA KKYVFYYGFS EAGGAGITTW ANQPYSGDFV IGQQRARKVV STDAMDAFAD
841: WPTVSEDFRF DAPSPSDVTW HRYTDEVRRV LKGCSEDVLG ILAERQEAMW AGIKALSDRK
901: ELLGSELRDI FDAHPAATSR DRDARAELAA AKLDMTIFTE GANSRWPYGI EWLDDAYPKP
961: YWVQQQEAEA AEAQAKQPAA AL