3.A.9.1.4
The 20 component Ycf2-FtsHi chloroplast protein import motor complex:
The Ycf2-FtsHi complex functions as a
TIC complex-associated ATPase motor in
chloroplast protein import. Cryo-EM
structure of the native Chlamydomonas reinhardtii Ycf2-FtsHi complex revealed up to 19 or 20
subunits, including conserved
heterohexameric motor components and
multiple green-algae-specific
components (). This offers evolutionary
insights into the conservation and
diversity of the chloroplast import motor
across species.
- •
Structure of the Chlamydomonas Ycf2-FtsHi complex revealed its composition and assembly.
- •
ATP binding induces a conformational changes in the ATPase domain of Ctap1.
- •
The preprotein interacts with Ycf2-FtsHi and enhances its ATPase activity in vitro.
- •
Ycf2-FtsHi exhibits evolutionary diversity between green algae and land plants,
|
| Accession Number: | 8XQX_F |
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| Protein Name: | F Chain F, Ctap6 |
|---|
| Length: | 1025 |
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| Molecular Weight: | |
|---|
| Species: | Chlamydomonas reinhardtii [3055] |
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| Number of TMSs: | 2 |
|---|
| Substrate |
protein |
|---|
1: MKATGLPSLP ARALGAAGCS TSPRPAALGW SSRGCASGRR RACARVHVAD AEAVASGVAA
61: TEAAAAVPAL PARATAVVAP LPEKNYGSLR GGRWPFLYDN VYGLPVVRQV ASYGEVLEGI
121: RTGRISQVLW FQAPRAVTAS AAAPPPGLGG PQQPQPPPLA SPDGRCLVRF ANGQVKQAVI
181: PPGEPRISQA LQQYGTAVSY IPLEPRYMPE LAAMRARGAQ EAVLGEVDTG AVATPVELPE
241: DERRGAAVGP TAFEAVAAYG SPEQLAAALD DNYQAAAGQV AALLAEREAW VAEQEALEAA
301: ARAERSMSDR AGGGGGGGGT ALVPSGGFSV GAWLDSIQLT NEQQAMVLKY VPILGPILGS
361: GFIIGLYLLA RLVKGDLTDR LKMMDSEADK KKKTALKEAR IAFLEEEVPG LVAKGASLDD
421: VRKRVQPVNA RLGTKLAIGD GEIQSTYEAC RLLLSEGVDL SAASSTAASG ALAQMESDER
481: RAAAGAAEGG GEGGDAMNAM MEMGKLNTAR IRKATDPKIM DVKKRVRDVR RKLKRESKVQ
541: LSDEIIFFDD IAGNKQAKVE LMEVVDFFRT PEKFKASGAR APKGVLLVGP PGNGKTLMAR
601: AVAGESGVAF ISSSAAEFIE MYMGLGAARV RDLFNTARSV APCIIFIDEL DAVGRQRQGG
661: GRSNDERDNT VNQLLTEMDG FEAEQQGIVV MGATNRKDVL DAALTRPGRF DRSIEVRRPD
721: FQGRLEAVKV HLRDKPVAAE IDYVSLASLM GGMSGAQIAG VANTACFLAS RDGRSEVNQT
781: DLTLAVEQAK YGRAYDQSRF VGAGRKKRFA VMEASIALAA TLLPAIEPVE YATIIPSTRS
841: PLGRTVLKPH VGRYTTGVWT YRYLREQLLV ALAGRAGEEL VLGRDELSSL NQHRLQMARQ
901: VAWKIMNSGM SSHPDYQHLR GLGSNYFDGS SEPGRFQQTT VVMDANQTRS EAVDADMEVE
961: GLLNGGYKQV FELLVRNRAA LDALTELLLE REKISGEEVV QVVEELGHPE DLARRAQWAG
1021: YELL