4.A.6.1.13 Fucosyl-α-1,3-N-acetylglucosamine PTS uptake porter, AlfEFG (next to an operon encoding a fucosidase (AlfB) and a transcriptional regulator of the GntR family, AlfR). The fucosidase is specific for this disaccharide
which is present in mammalian glycoproteins, glycolipids and milk (Rodríguez-Díaz et al. 2012). Uptake is dependent of AlfF but not on the PTS Enzyme I, suggesting that uptake does not require phosphorylation, consistent with the activity of AlfB as a fucosidase. Only the glucosamine moiety is utilized and the fucose moiety, after hydrolysis, is excreted (Rodríguez-Díaz et al. 2012).
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Accession Number: | B3WB10 |
Protein Name: | PTS system, IIC component |
Length: | 268 |
Molecular Weight: | 28959.00 |
Species: | Lactobacillus casei (strain BL23) [543734] |
Number of TMSs: | 6 |
Substrate |
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1: MAIAWWQILL LTMLAFWMII DQITVGILTN YPLLYGFVSG IIMGDVKTGL VVGATLQLMV
61: LGVGTYGGAS MPDFMTGAII GTAYAVISGK GINFAIGLAV PVGLLMVQLD VLARFTNTFF
121: LHRVDRYIEK GDTKRVARNI VYGVFPWGLS RAIPVFAMLV VGNTVVQKVV NAMPQWLSKG
181: LTAAGGILPV VGIAILLRFL PTKRFISYLV IGFLAAAYLK VPMLGVALLG LALAIIYYKQ
241: GSHQSSDHHD DPDTNKGGYE NAIGEYED