3.A.1.1.48 The lacto-N-biose I (LNB; Gal β-1,3-GlcNAc)/galacto-N-biose (GNB; Gal β-1,3-GalNAc) transporter. The solute-binding protein
crystallizes only in the presence of LNB or GNB, and it was therefore named GNB/LNB-binding protein (GL-BP) (Wada et al. 2007; Suzuki et al. 2008; Asakuma et al. 2011). Isothermal titration calorimetry
measurements revealed that GL-BP specifically binds LNB and GNB with
K(d) values of 0.087 and 0.010 μM, respectively, and the binding
process is enthalpy-driven. The crystal structures of GL-BP complexed
with LNB, GNB, and lacto-N-tetraose
(Galbeta1-3GlcNAcbeta1Suzuki et al. 2008; Asakuma et al. 2011). Isothermal titration calorimetry
measurements revealed that GL-BP specifically binds LNB and GNB with
K(d) values of 0.087 and 0.010 μM, respectively, and the binding
process is enthalpy-driven. The crystal structures of GL-BP complexed
with LNB, GNB, and lacto-N-tetraose
(Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc) were determined. The MalF and MalG membrahe proteinAsakuma et al. 2011). Isothermal titration calorimetry
measurements revealed that GL-BP specifically binds LNB and GNB with
K(d) values of 0.087 and 0.010 μM, respectively, and the binding
process is enthalpy-driven. The crystal structures of GL-BP complexed
with LNB, GNB, and lacto-N-tetraose
(Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc) were determined. The MalF and MalG membrahe proteins are encoded adjacent to the gene for GL-BP, but the ATPase was not identified.
|
Accession Number: | D6ZW64 |
Protein Name: | Extracellular solute-binding protein, family 1 |
Length: | 438 |
Molecular Weight: | 46255.00 |
Species: | Bifidobacterium longum subsp. longum (strain JDM301) [759350] |
Number of TMSs: | 1 |
Substrate |
beta-D-Galp-(1->3)-D-GlcpNAc |
---|
1: MVSHNKRIVA ALAAVAAMGI GLAGCGGSDT AGDTKSGSDG GVVNITYMHR LPDSEGMTLV
61: NDIVAKWNKE HPNIQVKATK FDGNAADMIK KLETDVKAGN APDLAQVGYS EVPEVFTKGL
121: LQDVTDEAAK YKDDFASGPF ALMQVDGKTY GLPQDTGPLT YFYNAAEFEK LGITVPKTAD
181: ELIETAKKTA AQGKYIMTFQ PDEAMMMMSG QAGASGPWYK VDGNSWVVNT QTKGSKAVAD
241: VYQQLIDNKA ALTNPRWDAS FDNSIQSGQL IGTVAAAWEA PLFIDSAGGT GAGEWKVTQL
301: GDWFGNGTKT GADGGSGVAV LKGSKHPAEA MEFLDWFNTQ VDDLVSQGLV VAATTADAKT
361: PQKWSDYFSG QDVMAEFKTA NDNMGDFTYM PGFSAVGAAM KQTAAKAADG SAKVSDVFDT
421: AQKTSVDTLK NLGLSVKE