1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
|
| Accession Number: | G0S4T0 |
|---|
| Protein Name: | Nucleoporin NUP192 |
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| Length: | 1756 |
|---|
| Molecular Weight: | 196928.00 |
|---|
| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
|---|
| Location1 / Topology2 / Orientation3: |
Nucleus1 |
|---|
| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MTDLRKLEAL QALHAELVAV RQHRFEGLQV LETLLEEQTD AFKALIAKPA RDTKDREALG
61: KEPKKLKIGE EEYSLNEDFV NDCLKLADEL DLNEKESARI LIDCDAEGDV ETQSRPLWEC
121: GVIRFHQERK YLLDCMRLIL EIAADEDIDA GLQESFGVAA EDKIFGIPPP WERNKENQPT
181: QVKKFIPRCM EAMKGVRSML QCMADKANAR NMLQQASLVR PLDNQETLDF SRLSLVEQHE
241: CLASILHAAV QRHHATIADF QDFIKILRKW DKYDHFLIHL IPVLAAYITE FGSPEGMGDL
301: QQARRLNDFI CKGGDEDSWA LPVLGAAVRA WWIAEHNGFY LDDTVQDLRG INLDEEDEQR
361: TKQFLDALKE GAFDFILSVA ADCKAQEWQD PSQLGARQWL QRKIPSLPSE PFPFSHFLQH
421: SLMVHLEGFV DATISNLPDV LRKLRTEEDE QRQLRPNHEQ DMDLERFLII ISYAYEGRPD
481: AAMSFWEDPD SNLAGFLQWA SRRASTPLVS AFCEMLRCLA DNEECATAAH NFLLDEGHQA
541: SGKMKRSQSL TWSQIFKELE YFTTKVCSER PNPPQASMHR PGRPGADPAE IEPESALMLE
601: CYLRLIAKLA TESEIARKRL IMDEDFNLVD TILKLSVGVI PHRLRACIFY VLKALMIRKT
661: HEELDAMWRW VEAWMTNPFS SLPGSQGAPQ RISFLGQTPG PQECMEMMFR EFGTGFEQSN
721: AFIQLLTTLL VPPEGLNSLN DSVPFPEWLG SSIRTLGIEP YVDFVFDVFA NRTKDISDPS
781: QLRILRLSCL DFVMVCLVTF NEDLIVLGHE SNISIDDAMA ATNLATYVRL HPFSRVMEWL
841: FNEKVITSLI NTIHQDPISL GSASPDSPLV VSILRAIQVM IKALELQETY LHLVRPEVLR
901: YQGEAGVRRK PVANAAYSAF EDGILSHLSL VVDLGKYCNL GHAELTLACL KLLEKISTSS
961: RILSAWSPDS GRLGHRNKAI VQLERNGEGE TISASLSASI MATLDPALAA SGENYRVKLA
1021: ILDFLYACLR ATPDQPTIAH QLLGFHCELS KLGIEPKGPF DMQKSLFHSL LNVLITLTVS
1081: EEEQGMRGYL VTLKYRVLRI LQLLWKSPLS ASLVMDELRA TNFLFHMLLR EVQIQPQLPW
1141: DGQLVTGCEF LLSDASLAYI DYLASRAAIF EYIGKELCSV SQNRIPSIKR QIFDALNGQI
1201: FVDEEAPLTI PSIFDFFDFI NTDYKWEEIP SPHFTYLKDL DLGPCILEHK YAGVHYDIRK
1261: AQEILALKRK EYEHSQLATP EFLETVELEE KVLIEWLTVR NRANLLLTAR LNLLQAWANL
1321: LLVMIESNDF KSTPKMAFLL QALQAILPTL EAFSSLKSDE AFELARVAKV LLWKLDFSQD
1381: SDAGLDREQF TVGNLIGDKL FQLFQLCLSA ISQCSGTPEL RSLYYSICYR YLTAVVDNDA
1441: TVAATPASST IGPTRSVTNA RARTLKAITL YGDRLLNVIC DDAYGSDTTC QTAAMILLNA
1501: LVHTSRASSA AGVSPADVDC PIIDALNRLN FIGVLVDSLK EILNEWLAPS STFDPSLSTN
1561: ASPSLPIPAS PSQQYTSAKL ALLLQLCQTR QGAKYVLQAN LFRALEQSGV FAADPELVEV
1621: DSESGVPRVV ALERHYALLV ALARVVGAAV TARGAHNIVQ GRKFLTQHRG LVVHVLKKNA
1681: GIGGGVVGNS LASSINGGST ATMTRRDEIL AQQALEERIE ELAEAFMLLI TATGFLEYES
1741: EQVPSEQPRA HTTFFH