1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
|
| Accession Number: | G0S7B6 |
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| Protein Name: | Nucleoporin NUP170 |
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| Length: | 1416 |
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| Molecular Weight: | 156249.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
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| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MEPMTPMRPI PGAYVNTPAP PTANPARRRL FTEASSSGAA ATTQLGAAPA PLASTMAPGP
61: EINSGLMTPQ AREDLPPVAK AAQVVNQTLQ LDDSYPDLDS YCRPGASSDY EMQSSDSSWA
121: PFHVVRHHNI PDKVFEHLNA GEVFTKLGLF AEIGYAWASI DSSLFLWDYT HPNPELIGYE
181: EATHTITAVA LVPPKPGVFV KTITHVLVVA TTSEIILLGV SATPTPSGSK SLTLYSTRMS
241: VHRGGSDVSF IVGTKDGRIF LGGESDTDIH EIFYQQEERW FSSRCGKINH SHPGWSAVVP
301: SLAGLPFGSR QQEWLRGLYV DDTRNLLYSL SNRSTIRTYH MEGPEKLTKV IEKDKTSCLR
361: DFAHMADSSP LFTDKTNIVA LSPIPATEAS KLHLMALTDT GCRLFLSATS SASYTMGGAT
421: SLAPQSMQLQ FVKFPPRESP TRIRTLNGQI IDSQLDKTSR ALDPSALGFR FSPGYFFDVV
481: RKHPNQDMLF VSAPDTGRIK VTQPASALKY FEQGTWIELE NGNRTIEIGL TTAPFAAAKQ
541: PLGFGNELAV QFDQVPGEFA VLTNTGVHIV RRRRLVDIFA KALGNCVSAS DDALEREVRK
601: FINQYGRVET IAAALAVACG QGSDLRTGTG RGMDRNTENL ARAAFIEYGG QPRLAESDGK
661: QSVSESVRLS SRHDALALYL TRLVRTLWKA KVVQVGSGSD ISSTIPTSKL VTIQENVERL
721: RNFLEANKST IQGLAPPSER LFGRQEDIAN QKEHQALHAL QKLMESISEG ISFVLMLFDE
781: RVSDIYARLD AVSQQQLKDL TYEQLFSQTP GKELAKVLVK AIVNRNIASG ANVETVADAL
841: RRRCGSFCSP DDVVTFKAQE QLQRASEQAH NSPVLRALLA ESLRLFEQVA GSLTPANLTT
901: AVEQYISLKY YAGAIQLCLT VAQQKDRGNT ALSWVNDGKP ANDSRKKAFD ERKICYNLIH
961: QVLDKLESDF AGEPELVDGR PTLAATKRME AYNVVNDSSD EVFHFDLYEW YIEKGWTDRI
1021: LSIDSPHVIT YLQRLAETDF RHAELLCRFY TTRSRFFEAA QVQTNLAKSD LNISLKDRII
1081: LLSRAKGNAS VNTIGISRQQ QQQLNHEASE LLEIAHIQDD LLERLVADPR IPEERKAEIE
1141: EFLDGPIRTL TDLFNDYADQ ANYYDLCLLI FHAADFHNPR TILDTWNNLI NQSHFEAEQR
1201: REYWEIVQAG GDLPAGVIAP IAEPPLPYVY VSQQIQLIAH RTSLDSLIFP VNSLLPVVCA
1261: YAINNGQDAS IGADPCWPIQ LFLNLGVPHA LVVQVLENVL DTQEAPFTGR RRKLVVQWIA
1321: MAVDMWVREV ERRGAMAAAA ASGASGSEAV MGSWVSELLG RADQVLTQIA GTGATLRGGA
1381: ASDAEEIASL RRTVKGLKRS VDMLLGGEMA RMSFFR