1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
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| Accession Number: | G0S9A7 |
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| Protein Name: | Nucleoporin NUP133 |
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| Length: | 1364 |
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| Molecular Weight: | 150745.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
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| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MFSSTLHEDG PATRTRSSRR RQRPVASDSS LQTQPKAKRQ RVPLTESNTA PTPTPADADA
61: PPEMFEVKPD PVLAKRERDG IGIENIENLG PRRELSLRSK KPKSGERTSK GDGSIILTTN
121: NAFTVSKLPA LPDRLRAEPT SRQHGAIFSS GYALALTHTH AFVWPYTATT ASPETFTFAL
181: PYPSKHASDP LPLGALVPPS ASSDDPGLVV VMPVSGRVVY WESISSAATL DFIRQQRTGI
241: EDAISGMYSS EHITQLVSAE SAGFVLVFSS GRIAYMSVRD PHGRPGITVQ YLRSPFGGAS
301: LGFLSTLRHA LSGSSRGDIA AAHANHGPRV GERVVIAASS KGRLQAWKIH RGGHHEPVAE
361: ADVRERLVEA VNEADAKTQA FPSESFEVLD FAFVPRGLEP KYVNASRLSE ALTHEEDSLQ
421: HMLLLVGFSR GHQARYSLVE TVLAPEGARI GTVRPITSYT SPVRPGALEK PRLYLPRPAL
481: VAFVVFDRAV VVASMVAPPD SPDSQLQEDS HILPPTFEDV VDFRDDDTLQ VVGSGSEEPG
541: AGTSSEDVRP HRHKTKNPTA VLLLPGVGIV RVAITDIERF ASDAPPRVTA KSKLEQAVFF
601: GIKSDNPLVF QGRRALPFSD REVCDAAVEL SHEIVNSKTP FIPSVPASLE GNMKSRSAYL
661: DALITFLNAC KVNMDERTRW MLLYDAEKMA VATWIWQKHE QFLAERPRAD KKTLISEAAV
721: FINENQKTEL NVAAGQVDPV RHWFIHDIFR LDIFVAWAYQ IIKYHYTQKL SDEPGLNRLV
781: WEAVTINNGA LLEARQFRLD KAAQYGVDPA VVPTGNGLPE PWTSTYFITN NLKRLTEFCH
841: QWLAKHDAQP SADPRFDARL LDTVRERLPS LTSQYFTSLS EYITWAASST DPETQDRCRA
901: YQAAYAEDVY KKIVKLKEFD LWEEAVELAR EFEAFDALAD VVVGQILMLE AAAADPTTTE
961: SKAQENAALA QVKKQRLGRL MEEFGEGFAS RAYEVLLDAA GVQAVLEFAF DRKGFITKWL
1021: RGKPELARIS WVNEVLREGD VGGAAETLLG LGMSREVQVW NKKVELSLGK LALLAEGGGS
1081: DDEAGKGEVG GTIKKIDAEL EVVKVQDLLY QWILASVHEA VDSSAEVELA VKQFGGLIPR
1141: RQKALLQIFE DGIARLLKHE VLDPLTLIDL LTLSSLGPGH YEGMGDQFFL ALKVAHYAAL
1201: ENAEEVRRLI WRRCLVRDDW RQVNETNLKG DEEALEAVGE TAAYRTLFAC FDEESSTPTF
1261: RPHHTLKPSD CLGVYTEPEQ LDSRFAAMDD SFRGKLVEAM RAEDRLLRGF VEKAQLDEWW
1321: RATRETAERM VGVAAQKGHR RVNSGSVGNG GVNGLSLNGR VKMY