1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
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| Accession Number: | G0SA56 |
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| Protein Name: | Protein MLP1 homolog |
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| Length: | 2085 |
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| Molecular Weight: | 231069.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
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| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MAAAEVDLGY LSAQANISQV DLETVVSAPT ADLVKSVLAA VLSKIRELEQ DKFHLNVELE
61: GAIRGAESRC EQFKATSDKA LKEVEELRQK LQSEESARRT LENELQTLKS SGSASLSEIE
121: TLRARIASLE TSNRETLAIV DSKASANAAL SEELQKQHQK ILKLNQEINN LNQAVQTAQT
181: AANSAKYREE SLKQELELAK KNNDWYDNEL KTKAAENLKI RKEKGAQIAQ LQREKEDALS
241: TIQSLQKTEQ QLRKRLQEAQ SKAEEALTKV QQLQESAARA EESFRQELES SKRLVELKDQ
301: QAQTHRNRLK EVELRLEKVK DDSAEEIRRV RRELEQAKED LSQSEQQVQD LQSEVDRLRT
361: LVESHDGVPG SVPQTPRANG SLLARPSSPF GTPASLRGKA TQRATETLEE LLKVKAQLAS
421: EQRRSQKLQE DLDDAVSMLE AKLPEIDELN AESERLRNEV IQMSEIMQQS YEERDAAVKA
481: ARKAEAAASQ AQAEVKILRA QLRDLSTQIH VLIFNAHAKE KGMDQLTEEE IAQFERLQRG
541: EISEGALEDL SDTHRFITER FTVFKDIYEL QQKNEELLKL TRELATKMEN EEALAAQRQA
601: AQEHEEVQQL RATVAALQDE VRSITIRMKS HMTERDMFRR MLQQRATPAE IQKVLGTQAD
661: GEQREVLPSV EQPHANEAQL AAALRELQAQ FDAYRNDQVT DRNAMREQID KLSGEKGSLQ
721: SEVTKLSSQL TLATERYNML ESNFKALQSE NQELQKRNQS LSEAAAKQDI RTQQVAEDLV
781: EARGLVESLR SENANLKAEK ALWRTIQERL TQDNESLAQE KTRLNGLLAS QQSLLNEREL
841: SEAETKRRLQ AQIDSLEAEL STTKRKLSEE IEESKKVQLR KEFDAQQFQK RIDELTSMIS
901: QVKEENIQVK TTRDHLQARV SELEIEVRNA QERAERLRPL PTPRAPAAAE QPSEEAQARI
961: EELEGEVQEL KNNLDLLTVQ LEHAKQQAEQ FKQLSKDMEE ELSSLNESHE QYRQEMDAAL
1021: ASKANTINEL QQRVEALTAE LSNTNNELNM LRDSQSDVAR KFEEKERMLN AEIARLKDEE
1081: ERYKEAARFH QQDLRAQAEI ATKAQQDYEQ ELVKHAEAAK LLQQLRAEHN ELKTQAAAWR
1141: AEADSAKISL AQSEQSWEER RQRLEQEIAE IKARRDDMAA QNKLLHQQLD AVTAQITALQ
1201: QKRTQGDVSG EAAAPAIADM ATEGLRELNS YLRREKEILE VQYDIKVQEA KRLQQQLEYT
1261: QSQLDETRLK LEQERASQAD STRTSLTHKE LMEKLNELNL IRESNVTLRN ENQRAQALLE
1321: QKAARITELE AKIQPLEARI AELELDKGFK EEEIRQLQEA RDGLQKRIET ILSKYGQADP
1381: QEVEQLKAMI AALEGERDVL KQSEQALQQK VKEAENALET KTNEWKATRE KLAEDFKARF
1441: RNMKTQRDEA TNEKNTLQAT LDGVKEQLAA LEKELETTKQ QLATATEKNT SLQQQLAASS
1501: TEQPAAAPVS AAPSDQINEL TQQLQAVKQQ LESVSAQKAA AEAQVEQLKQ ELAAAIAERD
1561: RALAATSGGD VATAETSVSA QPSAGLSDEE RKALEEKIAA AEAKAAEFEK RAKELEEQAD
1621: NIVKQRSDKM KTALNKKLQE SKEAMEKQIQ EEKAKLQAEF DLKLQQELAI IKAEQQTAGP
1681: QNGVPATPVK TEANAAPGTP VPDITNMTDA QIREAVAKNP TISAIVKSNV KRMVAAETKK
1741: IKEEIEAALK AEYEQKITNA KEQATALTEK KSALRINMLD RQHKTAQAKL AIVETAAKET
1801: PQKPVVEVWN IAKDAKPPAP AQAPAPAPAS AAPSPAPTPA QPVAPATAAP AAPAQAPSAA
1861: PPKEETKQEA KTTTAAPPSA IPKPAVNPFT APLNPFGAPA PTSNIPAPPQ AGQQPQQTQP
1921: QQPQQPQQQQ QQQKGQQQQQ QQQQQQQGQQ QQQQGQQQAG GQNQPQRMGI PVPAGRGGPG
1981: GARTARGLYQ AGPRGARGGR GGGFVGAGRG AGGAAGGLNP NAGEFTPGGA TATAAAAAAA
2041: GGAGGSAGAG NAGNKRQRDG EGGQPQGERG GKRARGGGGG GGGNQ