1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
|
| Accession Number: | G0SAK3 |
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| Protein Name: | Nucleoporin NUP145 |
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| Length: | 1793 |
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| Molecular Weight: | 188540.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
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| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
|---|
| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MSFGFGSGGF GQNNNSSTFG GFGSTPTTNT GFGSTGTTAF GSTSNTTGGG LFGGGGGGFG
61: SGNTFGSGFG SKPAFGTPAT TSSTSLFGST TTTAGGTGFG SGGFGSTNTS SPFGGGGTSL
121: FGNKTTTGFG SGTSTFGSNT GGGLFGGGST TTGFGATNNP GIGTNVGDPP GTAVVPFSPT
181: VEKEVNNPSQ SNSYQNILFM DAYKKWSAEE LRLADYNQGR KTAAPGGTGA FGSSGFGGFG
241: TTSNTGGFGS NTGGGLFGNT QQNTGGFGTT NTTGSAFGSG GGLFGNKPAT GGLFGTSSSQ
301: PAQSGGLFGS GTASTFGSSN TGTTSTFGSN NNTGGGLFGS NNTSSKPAFS FGTSNTSTPG
361: FGTATTGSGF GTGTTTNTGG GLFGNTAQNT NTGGGLFGNQ QQSGSAFGSG TGFGQQNQST
421: GTSLFGNTQQ KPGGLFGSTT TNTSGGLFGS TNTGTSTFGQ TPATQNTGGG LFGSKPAGTG
481: GLFGSTATNQ PASTGGLFGN LNTNAQTQQP ATGGLFGNLG QNNQAKPSLF GTSTTTGGGL
541: FGNTNAQQQT GSLFGTSTAQ QQPQTGLGAS LFGSSQQQQQ QPQTFSTSIT DISAYGATTL
601: FSGLPDDKIQ NPGPLATPLS GKAKVKSRSI LPMYKLSPAN ASRLVTTPQK RAYGFSFSAY
661: GSPTSPSSSA SSTPGAFGQS ILSSSINRGL NKSISASNLR RSLNVEDSIL QPGAFSANSS
721: MRLLGGPGSH KKLVINKDMR TDLFSPPNKD KQPQEDGTAA RKTVTKRVSF DTSNVETPEK
781: TIESSIPATD DSGYLKPDAR STANGTNGAN GAKSSPVAAA SPPEMEQVKG KELAVVHEEE
841: SPAPAQTDKP RGSQIEPGAY WMSPTADDIR AMNRMQRQRV VGFTVGRENV GSVQFKVPVD
901: LSNINLDDLF GTIVILEPRS ATVYPNAAKK PPMGKGLNVP ALISLEHSWP RGGPTIKGRR
961: LERHIERLKS IPDTTFESYD PETGVWAFSV EHFTTYGLGD DDDYDDDDYE TEPESAVKST
1021: PRPVTSPSIS KSSTSPIDPD DTFEFRRSRR ALPGAFDDAA LSDTDEVANH AQRQGTLSPE
1081: PQDADTPLPS REWPEDESMA DGLDEYQLEA YEEASQQGSV DEQEDFLPSR FAADNDAPQV
1141: PAGIMRARMR AVKKLNAPTK IEVAGGDDWT QILQASVKAP RTMDRATLRA LNESGAVWEM
1201: KDRGSPPPQA TATVSDGMGF ATSIDLMKSL FEQAKAPTQP ALTTSGKGFV KWPYEQRSKT
1261: DTEENLAVPR TNWGPNELLI STQHNEPNLL PVDAADDSAT SPSTLARLQQ YINLVSSKKQ
1321: LQRVAGPEFR ELAQGDSVWE LAALLFDDNG EGVSQFWQQL VSEATDRALS FTAGLEEKAI
1381: ICLAGNRVDE ACRHLLAAGN FRLATLVSTI GKVDNKDIRA QLKDWRESNV LAEFSEPIRA
1441: IYELLAGNAS VCAGVKNVPI ENRVNSFTIS QRFGLDWMRS FGLRLWYTSG VIPDVAAAVR
1501: SFQEDIEQDR EPEPDSALWT LLKAFASREY DWSDTRLGWL LTKAIYTTGK VSFGEDALQK
1561: LDKASVTFAS ALTAASHWVP ATFVLLQLSD PASREAAVRD HLGRHAHRIG SPRNLMSPFF
1621: TLQKFGVPEA WIWEAKALDY RSRQDSQQEF LALIWAQNYA EANRTFVTRV GPDLVIERNL
1681: PRLFAFAQLL FKVKKHLPNW ERSAAVYLLY PMAVMQNQGS GKLDRFDNQL IDGLVALHSQ
1741: THGDIRQEAA IADMAEELIK CKGAAAASDP RLLQLLPQDV RGKYLRAQVL EAF