1.I.1.1.2
Fungal Nuclear Pore Complex (NPC) with 29 components. Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015 presented
the reconstitution of the ~425-kilodalton inner ring
complex (IRC), which forms the central transport channel and diffusion
barrier of the NPC, revealing its interaction network and equimolar
stoichiometry. The Nsp1•Nup49•Nup57 channel nucleoporin heterotrimer
(CNT) attaches to the IRC solely through the adaptor nucleoporin Nic96.
The CNT•Nic96 structure reveals that Nic96 functions as an assembly
sensor that recognizes the three-dimensional architecture of the CNT,
thereby mediating the incorporation of a defined CNT state into the NPC.
They proposed that the IRC adopts a relatively rigid scaffold that
recruits the CNT to primarily form the diffusion barrier of the NPC,
rather than enabling channel dilation (Stuwe et al. 2015).
|
| Accession Number: | G0SFH5 |
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| Protein Name: | Nucleoporin NUP188 |
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| Length: | 1858 |
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| Molecular Weight: | 203809.00 |
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| Species: | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) [759272] |
|---|
| Number of TMSs: | 1 |
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| Location1 / Topology2 / Orientation3: |
Nucleus1 |
|---|
| Substrate |
protein polypeptide chain, (ribonucleotide)n+m |
|---|
1: MATLTDRTYL PPLEDCLTGR TVILSWRLVA SALEDADLAR LTSPALSTFL RDGFVHELLK
61: HPARVFEPKD LKQEFETKTS SIQTVAPGVD TIKKDALWLA DAVAINQVAA LRIVLIEYQT
121: RAHSHLVLPL STQDVANIQE AAGVGDAHAS SILSLLNPAS AVDAETMWCD FETEARRRER
181: ILATYLSERR SFTAAVDALV TFLLHSAPGQ HKDLDSLRRA LLKDAFAFDE DLDVPDRSKL
241: LTMAPTYMNL VEDCIARAQA LPAKLGESFK TEAFELDWLR TAITEAVHSL SIAFQALDLD
301: TPYFAPHELL SEWFELMNSS LFLESILGFE VVADLAMPAR SLVSAICLKM LNIDRTIQFL
361: HDFDYPDGEE PYLLSSQTLN KIHTAVTNAV NSGVAASLPV AFAWSLIVHQ MHLGYQERAE
421: RRDLLVNQRA QAGFELEFQP SASTPNRRRR NSAGSIVSLE ASPYDDFLRE QRLDNDIAPV
481: EQIAMLATSR GQVYQVMSEM ALCLGTTHEA AFRPAVGARA RLVFQDLLKR SAYLIPYQDE
541: PVFSLLAILA TGRQYWDVTD ALSASSLNQV YTDMLDDETL FTQFTMQAIN RFPYEFNPFS
601: VLCRVLAAAL ITNKDKADVV TGWLWRTPTL TVDWNPAWDR SYELCFEDEN TNSFRLTRDV
661: DLFGSASPAR PRHLAAEERF IIPEGTLGRF VTDVGRTARL EFEHSALALL GKRLEVKAAE
721: EICDSGMAPL DVDEQAEAVA MLATVLRAES LKSTAKGGDP EAPLKFLKEA SRLLPHNKDI
781: LTVISDTIDG LVEKELLELD GPQIAVLASC LQFLHAALAV CPGRVWAYMS RCALIAGDAR
841: PGRLSRITGS LDMYAERFDL LSSAVKLFAA LIDSAACSAV QRRAGSTALV SVRSAVENPW
901: LGTSEKILSR VALAIAQAAL DVYESTTTWR FRSELDRSIL VRDVVGLMHK LVVHAHTLSS
961: HLTSTLSPAA AHIISSFLTP PPSASSLRFQ PLLGTLLVAL ITPRATLYPG QSRILAERVT
1021: SVLAFCTSLL RAADFLGQTH IPLQTHLFQS ACLLARLPAA NAVYRAPVLE LLRALVEVAG
1081: RAANGSGEPP SLLGYLGSHA ARSFISLVEG IDKPFGRVEH AVVTWRFFAA VIRNRQQWMA
1141: GCLLTGRTPR EALKGGGEQK IERKVGEGSV LAAAMERLRE VKSLDVQEAV AVMDFVVSAQ
1201: NYWPWTIFAV RKEKEVVDAL RGYVRGLKAP GMVMKTDGAA AAAFQARIAA YVAETFAMQL
1261: YHMRQMRQAE KFAGELVADL DYFLREGVMV WGYNASLHGN FARNFAKRFP GVEVDDFKRT
1321: MWLPRELGKG YYYALEVAEQ MLGFDAGWGG VKQSGFRKEM ETANLNLSLV EAQVSLFHAW
1381: EYLLLELTLS LLPKKENAAF ARQVLQVVEQ CLEANQRSQP PENIFVVLGH ARAGLALTLL
1441: QRLADANQLP RDVTHLLALV SSAIHAVENP FGANDLPYFR TLLKILFVVL RAAKQGTAKP
1501: GESNVAITQQ VLTILDRVVA RCFRALAALV HEQQQNATDG TTTAPEDLAL ITAILQACLS
1561: VPGIEQCQVQ VLNIMAAHDV FQVAVALFSW ADRLLPANPS PASSSTSTSA TNPASGDPVY
1621: GELALLFLLE LSALPALAEH LACDGLLGHL AAARLAGYMR RTNVGPFAEN AGAARCYAIW
1681: AKCLLPLLLN ILAALGSTVA PEVAWVLNQF PNLLQSSVER IEPPGFSRPT LSLASTPPRQ
1741: KFISLLEISE IHSLALLTRV LAACRAQNAR DVPEVTWDGA KVLECVEYWL RGRKVLRERL
1801: VPLGPREVEW RGMVATGGVV GVAGDGGEGC ENRLEEKAVG LLVGVREVLE GGLEGEGE