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1.A.8.8.3
The Big Brain, BIB aquaporin of 696 aas and 6 TMSs, transports ions by a channel mechanism involving E71 in TMS1) (Yool, 2007).  BIB expressed in Xenopus oocytes is a monovalent cation channel modulated by tyrosine kinase signaling. BIB conductance shows voltage- and dose-dependent block by extracellular divalent cations Ca2+ and Ba2+ but not by Mg2+ in wild-type channels (Yanochko and Yool 2004). Site-directed mutagenesis of negatively charged glutamate (Glu274) and aspartate (Asp253) residues had no effect on divalent cation block, but mutation of Glu71 in the first TMS altered channel properties (Yanochko and Yool 2004).

Accession Number:P23645
Protein Name:BIB
Length:696
Molecular Weight:76509.00
Species:Drosophila melanogaster (Fruit fly) [7227]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate ion

Cross database links:

DIP: DIP-21188N
RefSeq: NP_476837.1   
Entrez Gene ID: 34330   
Pfam: PF00230   
KEGG: dme:Dmel_CG4722   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005261 F:cation channel activity
GO:0016337 P:cell-cell adhesion
GO:0007398 P:ectoderm development
GO:0006811 P:ion transport
GO:0007498 P:mesoderm development
GO:0007399 P:nervous system development
GO:0007219 P:Notch signaling pathway
GO:0030707 P:ovarian follicle cell development
GO:0055085 P:transmembrane transport

References (9)

[1] “Similarity of the product of the Drosophila neurogenic gene big brain to transmembrane channel proteins.”  Rao Y.et.al.   1692392
[2] “The genome sequence of Drosophila melanogaster.”  Adams M.D.et.al.   10731132
[3] “Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.”  Misra S.et.al.   12537572
[4] “A Drosophila full-length cDNA resource.”  Stapleton M.et.al.   12537569
[5] “The big brain gene of Drosophila functions to control the number of neuronal precursors in the peripheral nervous system.”  Rao Y.et.al.   1483394
[6] “The Drosophila neurogenic gene big brain, which encodes a membrane-associated protein, acts cell autonomously and can act synergistically with Notch and Delta.”  Doherty D.et.al.   9367444
[7] “Regulated cationic channel function in Xenopus oocytes expressing Drosophila big brain.”  Yanochko G.M.et.al.   11923418
[8] “Block by extracellular divalent cations of Drosophila big brain channels expressed in Xenopus oocytes.”  Yanochko G.M.et.al.   14990474
[9] “Phosphoproteome analysis of Drosophila melanogaster embryos.”  Zhai B.et.al.   18327897

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence 
1:	MADESLHTVP LEHNIDYHIV TLFERLEAMR KDSHGGGHGV NNRLSSTLQA PKRSMQAEIR 
61:	TLEFWRSIIS ECLASFMYVF IVCGAAAGVG VGASVSSVLL ATALASGLAM ATLTQCFLHI 
121:	SGAHINPAVT LALCVVRSIS PIRAAMYITA QCGGGIAGAA LLYGVTVPGY QGNLQAAISH 
181:	SAALAAWERF GVEFILTFLV VLCYFVSTDP MKKFMGNSAA SIGCAYSACC FVSMPYLNPA 
241:	RSLGPSFVLN KWDSHWVYWF GPLVGGMASG LVYEYIFNSR NRNLRHNKGS IDNDSSSIHS 
301:	EDELNYDMDM EKPNKYQQSQ GTYPRGQSNG NGGGQAAGNG QHQAANMGQM PGVVANAGQG 
361:	NYCQNLYTAP PLSSKYDQQQ EPLYGGTRSL YCRSPTLTRS NLNRSQSVYA KSNTAINRDI 
421:	VPRPGPLVPA QSLYPMRTQQ QQQQQQQQQQ QVAPAPQSSH LQNQNVQNQM QQRSESIYGM 
481:	RGSMRGQQQP IQQQQQQQQQ QQLQQQQPNM GVQQQQMQPP PQMMSDPQQQ PQGFQPVYGT 
541:	RTNPTPMDGN HKYDRRDPQQ MYGVTGPRNR GQSAQSDDSS YGSYHGSAVT PPARHPSVEP 
601:	SPPPPPMLMY APPPQPNAAH PQPIRTQSER KVSAPVVVSQ PAACAVTYTT SQGSAVTAQQ 
661:	QQQQQQQQQQ QQQQQQQQMM MQQQQQHYGM LPLRPN