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1.B.19 The Glucose-selective OprB Porin (OprB) Family

The OprB family of outer membrane porins includes proteins from α-, β-, γ- and δ-proteobacteria including species of Pseudomonas, Acinetobacter, Xanthomonas, Ralstonia, Bradyrhizobium, and other organisms including cyanobacteria, flavobacteria, planctomyces and chlamydia. Some of the Pseudomonas homologues have been reconstituted in proteoliposomes and characterized (Adewoye et al., 1998). The Km for glucose of the P. aeruginosa homologue is 4 μM. It exhibits high specificity for glucose, but also transports glycerol, mannitol and fructose (Wylie and Worobec, 1993). They have 40-50% β-structure. The oprB gene is adjacent to the gltK gene encoding an ATPase of a probable glucose ABC transporter. oprB expression is induced by glycerol and glucose limitation, repressed by salicylate, and responsive to temperature and pH (Adewoye and Worobec, 1999).

A second member of the family is OprB of Burkholderia pseudomallei (TC #1.B.19.1.2; Chan et al., 2007). This functions in conjunction with BpeAB (TC #2.A.6.2.23) to export several quorum sensing acyl homoserine lactones with acyl chains of 8-14 carbons.

References associated with 1.B.19 family:

Adewoye, L.O. and E.A. Worobec. (2000). Identification and characterization of the gltK gene encoding a membrane-associated glucose transport protein of Pseudomonas aeruginosa. Gene 253: 323-330. 10940570
Adewoye, L.O. and E.A. Worobec. (1999). Multiple environmental factors regulate the expression of the carbohydrate-selective OprB porin of Pseudomonas aeruginosa. Can. J. Microbiol. 45: 1033-1042. 10696483
Adewoye, L.O., L. Tschetter, J. O'Neil, and E.A. Worobec. (1998). Channel specificity and secondary structure of the glucose-inducible porins of Pseudomonas spp. J. Bioenerg. Biomembr. 30: 257-267. 9733092
Bae, N., H.J. Park, H. Park, M. Kim, and S.W. Han. (2018). Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation, and stress tolerance in Xanthomonas oryzae pv. oryzae. Mol Plant Pathol. [Epub: Ahead of Print] 30073749
Chan, Y.Y., H.S. Bian, T.M. Tan, M.E. Mattmann, G.D. Geske, J. Igarashi, T. Hatano, H. Suga, H.E. Blackwell, and K.L. Chua. (2007). Control of quorum sensing by a Burkholderia pseudomallei multidrug efflux pump. J. Bacteriol. 189: 4320-4324. 17384185
Ficarra, F.A., C. Grandellis, E.M. Galván, L. Ielpi, R. Feil, J.E. Lunn, N. Gottig, and J. Ottado. (2016). Xanthomonas citri subsp. citri requires the outer membrane porin OprB for maximal virulence and biofilm formation. Mol Plant Pathol. [Epub: Ahead of Print] 27226289
Saravolac, E.G., N.F. Taylor, R. Benz, and R.E. Hancock. (1991). Purification of glucose-inducible outer membrane protein OprB of Pseudomonas putida and reconstitution of glucose-specific pores. J. Bacteriol. 173: 4970-4976. 1650338
Shrivastava, R., B. Basu, A. Godbole, M.K. Mathew, S.K. Apte, and P.S. Phale. (2011). Repression of the glucose-inducible outer-membrane protein OprB during utilization of aromatic compounds and organic acids in Pseudomonas putida CSV86. Microbiology 157: 1531-1540. 21330430
van den Berg, B. (2012). Structural basis for outer membrane sugar uptake in pseudomonads. J. Biol. Chem. 287: 41044-41052. 23066028
Williams, S.G., J.A. Greenwood, and C.W. Jones. (1994). The effect of nutrient limitation on glycerol uptake and metabolism in continuous cultures of Pseudomonas aeruginosa. Microbiology 140: 2961-2969. 7812436
Wylie, J.L. and E.A. Worobec. (1993). Substrate specificity of the high-affinity glucose transport system of Pseudomonas aeruginosa. Can. J. Microbiol. 39: 722-725. 8395965
Wylie, J.L. and E.A. Worobec. (1994). Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae. Eur. J. Biochem. 220: 505-512. 8125108