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1.C.31 The Channel-forming Colicin V (Colicin V) Family

Colicin (Microcin) V is unrelated to other channel-forming colicins (e.g., the Colicin family; TC #1.C.1). The protein is much smaller (103 amino acyl residues) than the other colicins, and it therefore does not form the complex structure characteristic of the homologous members of the colicin family. Colicin V is, however, a pore-forming bacteriocin which exerts its action by inserting into the cytoplasmic membranes of target bacteria and dissipating the membrane potential while facilitating leakage of small molecules from the cytoplasm. In these functional respects (but not structural respects) it therefore resembles channel-forming bacteriocins that are included in the channel-forming amphipathic peptide (CAP) functional superfamily which includes defensins and bacteriocins (e.g., TC families 1.C.16-1.C.30). Colicin V is demonstrably homologous to other proteins such as Microcin L (AAP08989; 105 aas) and the McmA protein (CAD56188; 92 aas), both of E. coli. Colicin V has been shown to depend on the inner membrane SdaC protein (2.A.42.2.1) for its bacteriocidal activity (Gérard et al., 2005). SdaC presumably serves as an inner membrane receptor for Colicin V.

The generalized transport reaction catalyzed by Colicin V is:

Ions and small molecules (in) ions and small molecules (out)

This family belongs to the: Bacterial Bacteriocin (BB) Superfamily.

References associated with 1.C.31 family:

Boubezari, M.T., T. Idoui, R. Hammami, B. Fernandez, A. Gomaa, and I. Fliss. (2018). Bacteriocinogenic properties of Escherichia coli P2C isolated from pig gastrointestinal tract: purification and characterization of microcin V. Arch. Microbiol. 200: 771-782. 29417164
Gérard, F., N. Pradel, and L.-F. Wu. (2005). Bactericidal activity of Colicin V is mediated by an inner membrane protein, SdaC, of Escherichia coli. J. Bacteriol. 187: 1945-1950. 15743941
Gilson, L., H.K. Mahanty and R. Kolter (1990). Genetic analysis of an MDR-like export system: the secretion of colicin V. EMBO J. 9: 3875-94. 2249654
Sablé, S., M. Duarte, D. Bravo, I. Lanneluc, A.M. Pons, G. Cottenceau, and F. Moreno. (2003). Wild-type Escherichia coli producing microcins B17, D93, J25, and L; cloning of genes for microcin L production and immunity. Can. J. Microbiol. 49: 357-361. 12897830