1.C.78 The Crystal Protein (Cry) Family
Crystal proteins of Bacillus thuringiensis form pores in lepidopteran larvae and have insecticidal activity (Brown and Whiteley, 1992; Okumura et al., 2006). They form discrete families, some of which are related to other cytotoxins from a variety of bacteria.
The generalized transport reaction is:
ions (in) ions (out)
This family belongs to the: Aerolysin Superfamily.
References associated with 1.C.78 family:
Brown K.L. and H.R. Whiteley. (1992). Molecular Characterization of Two Novel Crystal Protein Genes from Bacillus thuringiensis subsp. thompsoni. J. Bacteriol. 174: 549-557. 1729243
Moar, W.J., A.J. Evans, C.R. Kessenich, J.A. Baum, D.J. Bowen, T.C. Edrington, J.A. Haas, J.K. Kouadio, J.K. Roberts, A. Silvanovich, Y. Yin, B.E. Weiner, K.C. Glenn, and M.L. Odegaard. (2016). The sequence, structural, and functional diversity within a protein family and implications for specificity and safety: The case for ETX_MTX2 insecticidal proteins. J Invertebr Pathol. [Epub: Ahead of Print] 27235983
Okumura S., H. Saitoh, N. Wasano, H. Katayama, K. Higuchi, E. Mizuki, K. Inouye. (2006). Efficient solubilization, activation, and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as inclusion bodies in Escherichia coli. Protein Expr. Purif. 47: 144-51. 16307894
Xu, C., U. Chinte, L. Chen, Q. Yao, Y. Meng, D. Zhou, L.J. Bi, J. Rose, M.J. Adang, B.C. Wang, Z. Yu, and M. Sun. (2015). Crystal structure of Cry51Aa1: A potential novel insecticidal aerolysin-type β-pore-forming toxin from Bacillus thuringiensis. Biochem. Biophys. Res. Commun. 462: 184-189. 25957471