Holin (gene 44) of Pseudomonas phage phiKMV of 66 aas and 1 TMS.  The holin accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of nanometer-scale pores (pinholes) causing host cell membrane depolarization and a protein, endolysin orcytolysin refolding and release into the periplasmic space (Briers et al. 2011). The 66 aa holin from Pseudomonas phage AIIMS-Pa-Ai (genbank acc # QPP21131.1) is identical to this one (Rathor et al. 2022). An identical protein in the region of overlap has 77 aas (Rathor et al. 2022).