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3.A.1.103.9
ABC O-antigen lipopolysaccharide/polysaccharide export transporter, Wzm/Wzt of 253 aas and 6 TMSs (Wzm; also called AbcT3) and 396 aas and 0 TMSs (Wzt). The crystal structure is available (PDB 6AN7) (Bi et al. 2018) for the Wzm-Wzt homologue from Aquifex aeolicus in an open conformation. The transporter forms a transmembrane channel that is sufficiently wide to accommodate a linear polysaccharide. Its nucleotide-binding domain and a periplasmic extension form 'gate helices' at the cytosolic and periplasmic membrane interfaces that probably serve as substrate entry and exit points (Bi et al. 2018). O antigen structures are serotype specific and form extended cell surface barriers endowing many pathogens with survival benefits. In the ABC transporter-dependent biosynthesis pathway, O antigens are assembled on the cytosolic side of the inner membrane on a lipid anchor and reoriented to the periplasmic leaflet by WzmWzt for ligation to the core lipopolysaccharides. This process depends on the chemical modification of the O antigen's nonreducing terminus, sensed by WzmWzt via a carbohydrate-binding domain (CBD) that extends its nucleotide-binding domain (NBD). Caffalette and Zimmer 2021 provided the cryoEM structure of this full-length WzmWzt transporter bound to ATP in a lipid environment, revealing a highly asymmetric transporter organization. The CBDs dimerize and associate with only one NBD. Conserved loops at the CBD dimer interface straddle a conserved peripheral NBD helix. The CBD dimer is oriented perpendicularly to the NBDs and its putative ligand-binding sites face the transporter to likely modulate ATPase activity upon O antigen binding. A closed WzmWzt conformation in which an aromatic belt near the periplasmic channel exit seals the transporter in a resting, ATP-bound state. The sealed transmembrane channel is asymmetric, with one open and one closed cytosolic and periplasmic portal (Caffalette and Zimmer 2021).   

Accession Number:O67182
Protein Name:Transport permease protein
Length:256
Molecular Weight:30005.00
Species:Aquifex aeolicus (strain VF5) [224324]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate

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Structure:
6M96   6OIH     

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FASTA formatted sequence
1:	MNLSLILELV RQEIKNRYAD TVLGIWWAFL WPILLVLIYT LIFSHLIGAK LGHENTVYAY 
61:	SIYLSSGIFP WFFFSNSLSR ITGIFTEKKF LFTKIPIRLE VFPVVVIISE LINYLIGISL 
121:	VTLISFITLG FEGIKYFYLF PVALYLMIVY SFSIGMVLGT LNVFFRDIKE IIGVFLQIFF 
181:	WFTPIVYTLD ILPPFVKKLI YYNPMYPVVS IHHLVFVNYL DLHLYSLLGF LLASPLVFFV 
241:	SYYFFKKLEK DIKDFA